Coxiella burnetii in slaughterhouses in Brazil: A public health concern
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0241246 http://hdl.handle.net/11449/209640 |
Resumo: | Q fever is an important zoonosis, yet it is often neglected and can present large outbreaks, as observed in the Netherlands. In the past few years, cases of Q fever have been described in Brazil; however, the epidemiological situation of Q fever in ruminants, the main reservoir of the pathogen, is unknown in this country. Our study aimed to estimate the prevalence of C. burnetii in cattle sent to slaughterhouses using an immunofluorescence assay (IFA) and quantitative real-time PCR (qPCR). From 1515 cattle serum samples collected from nine slaughterhouses, 23.8% (360/1515) were serologically positive by IFA (cutoff titer>1:64), indicating past or recent exposure to C. burnetii infection. Among the 54 cities sampled during the study, 83.3% (45/54) had at least one seropositive animal. Subsequently, all seropositive samples were submitted to qPCR for C. burnetii DNA, and 12.2% (44/360) of the sera were qPCR positive, which indicates bacteremia and suggests active or recent infection. The results highlight the risk for abattoir workers that results from exposure to contaminated aerosols produced during slaughter procedures. Moreover, the heat maps that were construction from the positive samples demonstrate the widespread distribution of C. burnetii in the State of Sao Paulo, Brazil and denotes the need for surveillance and preventive measures to reduce the prevalence in cattle. |
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Coxiella burnetii in slaughterhouses in Brazil: A public health concernQ fever is an important zoonosis, yet it is often neglected and can present large outbreaks, as observed in the Netherlands. In the past few years, cases of Q fever have been described in Brazil; however, the epidemiological situation of Q fever in ruminants, the main reservoir of the pathogen, is unknown in this country. Our study aimed to estimate the prevalence of C. burnetii in cattle sent to slaughterhouses using an immunofluorescence assay (IFA) and quantitative real-time PCR (qPCR). From 1515 cattle serum samples collected from nine slaughterhouses, 23.8% (360/1515) were serologically positive by IFA (cutoff titer>1:64), indicating past or recent exposure to C. burnetii infection. Among the 54 cities sampled during the study, 83.3% (45/54) had at least one seropositive animal. Subsequently, all seropositive samples were submitted to qPCR for C. burnetii DNA, and 12.2% (44/360) of the sera were qPCR positive, which indicates bacteremia and suggests active or recent infection. The results highlight the risk for abattoir workers that results from exposure to contaminated aerosols produced during slaughter procedures. Moreover, the heat maps that were construction from the positive samples demonstrate the widespread distribution of C. burnetii in the State of Sao Paulo, Brazil and denotes the need for surveillance and preventive measures to reduce the prevalence in cattle.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Higiene Vet & Saude Publ, Botucatu, SP, BrazilUniv Estadual Maranhao, Dept Patol, Sao Luis, Maranhao, BrazilUniv Sao Paulo, Inst Quim, Sao Paulo, SP, BrazilUniv Sao Paulo, Dept Med Vet Prevent & Saude Anim, Sao Paulo, SP, BrazilFrench Agcy Food Environm & Occupat Hlth Safety, Sophia Antipolis Lab, Anses, Anim Q Fever Unit, Sophia Antipolis, FranceUniv Estadual Paulista, Dept Higiene Vet & Saude Publ, Botucatu, SP, BrazilCAPES: 88887.137563/2017-00CAPES: 88882.180556/2018-01FAPESP: 2019/05300-9Public Library ScienceUniversidade Estadual Paulista (Unesp)Univ Estadual MaranhaoUniversidade de São Paulo (USP)French Agcy Food Environm & Occupat Hlth SafetyRibeiro Mioni, Mateus de Souza [UNESP]Costa, Francisco BorgesDevide Ribeiro, Bruna Leticia [UNESP]Reis Teixeira, Wanderson Sirley [UNESP]Pelicia, Vanessa Cristina [UNESP]Labruna, Marcelo BahiaRousset, ElodieSidi-Boumedine, KarimThiery, RichardMegid, Jane [UNESP]2021-06-25T12:24:45Z2021-06-25T12:24:45Z2020-10-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.1371/journal.pone.0241246Plos One. San Francisco: Public Library Science, v. 15, n. 10, 14 p., 2020.1932-6203http://hdl.handle.net/11449/20964010.1371/journal.pone.0241246WOS:000588368900010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos Oneinfo:eu-repo/semantics/openAccess2021-10-23T19:28:26Zoai:repositorio.unesp.br:11449/209640Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:28:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Coxiella burnetii in slaughterhouses in Brazil: A public health concern |
title |
Coxiella burnetii in slaughterhouses in Brazil: A public health concern |
spellingShingle |
Coxiella burnetii in slaughterhouses in Brazil: A public health concern Ribeiro Mioni, Mateus de Souza [UNESP] |
title_short |
Coxiella burnetii in slaughterhouses in Brazil: A public health concern |
title_full |
Coxiella burnetii in slaughterhouses in Brazil: A public health concern |
title_fullStr |
Coxiella burnetii in slaughterhouses in Brazil: A public health concern |
title_full_unstemmed |
Coxiella burnetii in slaughterhouses in Brazil: A public health concern |
title_sort |
Coxiella burnetii in slaughterhouses in Brazil: A public health concern |
author |
Ribeiro Mioni, Mateus de Souza [UNESP] |
author_facet |
Ribeiro Mioni, Mateus de Souza [UNESP] Costa, Francisco Borges Devide Ribeiro, Bruna Leticia [UNESP] Reis Teixeira, Wanderson Sirley [UNESP] Pelicia, Vanessa Cristina [UNESP] Labruna, Marcelo Bahia Rousset, Elodie Sidi-Boumedine, Karim Thiery, Richard Megid, Jane [UNESP] |
author_role |
author |
author2 |
Costa, Francisco Borges Devide Ribeiro, Bruna Leticia [UNESP] Reis Teixeira, Wanderson Sirley [UNESP] Pelicia, Vanessa Cristina [UNESP] Labruna, Marcelo Bahia Rousset, Elodie Sidi-Boumedine, Karim Thiery, Richard Megid, Jane [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Estadual Maranhao Universidade de São Paulo (USP) French Agcy Food Environm & Occupat Hlth Safety |
dc.contributor.author.fl_str_mv |
Ribeiro Mioni, Mateus de Souza [UNESP] Costa, Francisco Borges Devide Ribeiro, Bruna Leticia [UNESP] Reis Teixeira, Wanderson Sirley [UNESP] Pelicia, Vanessa Cristina [UNESP] Labruna, Marcelo Bahia Rousset, Elodie Sidi-Boumedine, Karim Thiery, Richard Megid, Jane [UNESP] |
description |
Q fever is an important zoonosis, yet it is often neglected and can present large outbreaks, as observed in the Netherlands. In the past few years, cases of Q fever have been described in Brazil; however, the epidemiological situation of Q fever in ruminants, the main reservoir of the pathogen, is unknown in this country. Our study aimed to estimate the prevalence of C. burnetii in cattle sent to slaughterhouses using an immunofluorescence assay (IFA) and quantitative real-time PCR (qPCR). From 1515 cattle serum samples collected from nine slaughterhouses, 23.8% (360/1515) were serologically positive by IFA (cutoff titer>1:64), indicating past or recent exposure to C. burnetii infection. Among the 54 cities sampled during the study, 83.3% (45/54) had at least one seropositive animal. Subsequently, all seropositive samples were submitted to qPCR for C. burnetii DNA, and 12.2% (44/360) of the sera were qPCR positive, which indicates bacteremia and suggests active or recent infection. The results highlight the risk for abattoir workers that results from exposure to contaminated aerosols produced during slaughter procedures. Moreover, the heat maps that were construction from the positive samples demonstrate the widespread distribution of C. burnetii in the State of Sao Paulo, Brazil and denotes the need for surveillance and preventive measures to reduce the prevalence in cattle. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-30 2021-06-25T12:24:45Z 2021-06-25T12:24:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0241246 Plos One. San Francisco: Public Library Science, v. 15, n. 10, 14 p., 2020. 1932-6203 http://hdl.handle.net/11449/209640 10.1371/journal.pone.0241246 WOS:000588368900010 |
url |
http://dx.doi.org/10.1371/journal.pone.0241246 http://hdl.handle.net/11449/209640 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 15, n. 10, 14 p., 2020. 1932-6203 10.1371/journal.pone.0241246 WOS:000588368900010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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