Surveillance of canine visceral leishmaniasis in a disease-free area
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4322/rbpv.01901011 http://hdl.handle.net/11449/141873 |
Resumo: | Leishmaniasis is an important re-emergent parasitosis worldwide, particularly in tropical countries. There are no reports of autochthonous disease in the State of Paraná, southern Brazil. No surveillance has been carried out in the most populated areas such as the city of Curitiba and its surroundings. The purpose of the present study was to determine the seroprevalence of visceral leishmaniasis in dogs at the Center for Zoonosis Control of São José dos Pinhais, Paraná, before euthanasia. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT) were used to detect antibody levels against Leishmania sp. in dog sera. Imprints of the popliteal lymph nodes that were also randomly collected from 50 dogs with suspected clinical signs of visceral leishmaniasis, and evaluated under light microscopy for the detection of amastigote forms, were negative. A total of 364 dog samples were tested. The results showed only one positive sample (0.0027%) by ELISA test but negative by IFAT, however, the dog had no clinical signs. Random surveillance of dog populations from several districts of a metropolitan area may be a means of preventing Leishmania spreading. Based on our results, the city of Curitiba and its metropolitan area were considered at low risk for visceral leishmaniasis. |
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Surveillance of canine visceral leishmaniasis in a disease-free areaVigilância da leishmaniose visceral canina em área indeneLeishmania sp.ELISARIFIdogzoonosesLeishmania sp.ELISARIFIcãozoonosesLeishmaniasis is an important re-emergent parasitosis worldwide, particularly in tropical countries. There are no reports of autochthonous disease in the State of Paraná, southern Brazil. No surveillance has been carried out in the most populated areas such as the city of Curitiba and its surroundings. The purpose of the present study was to determine the seroprevalence of visceral leishmaniasis in dogs at the Center for Zoonosis Control of São José dos Pinhais, Paraná, before euthanasia. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT) were used to detect antibody levels against Leishmania sp. in dog sera. Imprints of the popliteal lymph nodes that were also randomly collected from 50 dogs with suspected clinical signs of visceral leishmaniasis, and evaluated under light microscopy for the detection of amastigote forms, were negative. A total of 364 dog samples were tested. The results showed only one positive sample (0.0027%) by ELISA test but negative by IFAT, however, the dog had no clinical signs. Random surveillance of dog populations from several districts of a metropolitan area may be a means of preventing Leishmania spreading. Based on our results, the city of Curitiba and its metropolitan area were considered at low risk for visceral leishmaniasis.A leishmaniose é uma importante parasitose re-emergente observada no mundo, particularmente em países tropicais. Não há ainda relatos de casos autóctones no estado do Paraná. Não há até o momento referência de vigilância no reservatório canino, tais como Curitiba e região metropolitana do estado. O objetivo do estudo foi determinar a soroprevalência da leishmaniose visceral em cães entregues ao Centro de Controle de Zoonoses de São José dos Pinhais, Paraná para eutanásia. A detecção sorológica da presença de anticorpos contra Leishmania sp. foi realizada por (ELISA) indireto e pela Reação de Imunofluorescência Indireta (RIFI). Além disso, impressão de linfonodo poplíteo coletadas ao acaso de 50 cães com sinais clínicos suspeitos para leishmaniose visceral e analisados sob microscopia óptica para detecção de formas amastigotas, foram negativas. Amostras de soro de 364 animais foram testadas, e os resultados mostraram somente uma amostra positiva (0,0027%), reagente ao ELISA e negativa à RIFI, entretanto, o cão não apresentava sinais clínicos. A vigilância ao acaso em uma população de vários locais de uma área metropolitana pode ser uma forma de prevenção da disseminação da doença. Com base nos resultados observados, Curitiba e região metropolitana foram consideradas de baixo risco para a leishmaniose visceral.Universidade Federal do Paraná (UFPR), Departameno de Medicina Veterinária, Curitiba, PR, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Higiene Veterinária e Saúde Pública, Botucatu, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Higiene Veterinária e Saúde Pública, Botucatu, SP, BrasilColégio Brasileiro de Parasitologia VeterináriaUniversidade Federal do Paraná (UFPR)Universidade Estadual Paulista (Unesp)Frehse, Michele SalmonGreca Júnior, Haroldo [UNESP]Ullmann, Leila Sabrina [UNESP]Camossi, Lucilene Granuzzio [UNESP]Machado, Juliana G. [UNESP]Langoni, Hélio [UNESP]Biondo, Alexander WelkerMolento, Marcelo Beltrão2016-07-19T13:51:16Z2016-07-19T13:51:16Z2010-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article62-64application/pdfhttp://dx.doi.org/10.4322/rbpv.01901011Revista Brasileira de Parasitologia Veterinária. Colégio Brasileiro de Parasitologia Veterinária, v. 19, n. 1, p. 62-64, 2010.1984-2961http://hdl.handle.net/11449/14187310.4322/rbpv.01901011S1984-29612010000100012WOS:000284796300012S1984-29612010000100012.pdf5326072118518067SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinária1.090info:eu-repo/semantics/openAccess2024-01-02T06:19:31Zoai:repositorio.unesp.br:11449/141873Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-02T06:19:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Surveillance of canine visceral leishmaniasis in a disease-free area Vigilância da leishmaniose visceral canina em área indene |
title |
Surveillance of canine visceral leishmaniasis in a disease-free area |
spellingShingle |
Surveillance of canine visceral leishmaniasis in a disease-free area Frehse, Michele Salmon Leishmania sp. ELISA RIFI dog zoonoses Leishmania sp. ELISA RIFI cão zoonoses |
title_short |
Surveillance of canine visceral leishmaniasis in a disease-free area |
title_full |
Surveillance of canine visceral leishmaniasis in a disease-free area |
title_fullStr |
Surveillance of canine visceral leishmaniasis in a disease-free area |
title_full_unstemmed |
Surveillance of canine visceral leishmaniasis in a disease-free area |
title_sort |
Surveillance of canine visceral leishmaniasis in a disease-free area |
author |
Frehse, Michele Salmon |
author_facet |
Frehse, Michele Salmon Greca Júnior, Haroldo [UNESP] Ullmann, Leila Sabrina [UNESP] Camossi, Lucilene Granuzzio [UNESP] Machado, Juliana G. [UNESP] Langoni, Hélio [UNESP] Biondo, Alexander Welker Molento, Marcelo Beltrão |
author_role |
author |
author2 |
Greca Júnior, Haroldo [UNESP] Ullmann, Leila Sabrina [UNESP] Camossi, Lucilene Granuzzio [UNESP] Machado, Juliana G. [UNESP] Langoni, Hélio [UNESP] Biondo, Alexander Welker Molento, Marcelo Beltrão |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná (UFPR) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Frehse, Michele Salmon Greca Júnior, Haroldo [UNESP] Ullmann, Leila Sabrina [UNESP] Camossi, Lucilene Granuzzio [UNESP] Machado, Juliana G. [UNESP] Langoni, Hélio [UNESP] Biondo, Alexander Welker Molento, Marcelo Beltrão |
dc.subject.por.fl_str_mv |
Leishmania sp. ELISA RIFI dog zoonoses Leishmania sp. ELISA RIFI cão zoonoses |
topic |
Leishmania sp. ELISA RIFI dog zoonoses Leishmania sp. ELISA RIFI cão zoonoses |
description |
Leishmaniasis is an important re-emergent parasitosis worldwide, particularly in tropical countries. There are no reports of autochthonous disease in the State of Paraná, southern Brazil. No surveillance has been carried out in the most populated areas such as the city of Curitiba and its surroundings. The purpose of the present study was to determine the seroprevalence of visceral leishmaniasis in dogs at the Center for Zoonosis Control of São José dos Pinhais, Paraná, before euthanasia. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT) were used to detect antibody levels against Leishmania sp. in dog sera. Imprints of the popliteal lymph nodes that were also randomly collected from 50 dogs with suspected clinical signs of visceral leishmaniasis, and evaluated under light microscopy for the detection of amastigote forms, were negative. A total of 364 dog samples were tested. The results showed only one positive sample (0.0027%) by ELISA test but negative by IFAT, however, the dog had no clinical signs. Random surveillance of dog populations from several districts of a metropolitan area may be a means of preventing Leishmania spreading. Based on our results, the city of Curitiba and its metropolitan area were considered at low risk for visceral leishmaniasis. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-01 2016-07-19T13:51:16Z 2016-07-19T13:51:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4322/rbpv.01901011 Revista Brasileira de Parasitologia Veterinária. Colégio Brasileiro de Parasitologia Veterinária, v. 19, n. 1, p. 62-64, 2010. 1984-2961 http://hdl.handle.net/11449/141873 10.4322/rbpv.01901011 S1984-29612010000100012 WOS:000284796300012 S1984-29612010000100012.pdf 5326072118518067 |
url |
http://dx.doi.org/10.4322/rbpv.01901011 http://hdl.handle.net/11449/141873 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinária. Colégio Brasileiro de Parasitologia Veterinária, v. 19, n. 1, p. 62-64, 2010. 1984-2961 10.4322/rbpv.01901011 S1984-29612010000100012 WOS:000284796300012 S1984-29612010000100012.pdf 5326072118518067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária 1.090 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
62-64 application/pdf |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965488476848128 |