Polyspecies biofilm formation on implant surfaces with different surface characteristics

Detalhes bibliográficos
Autor(a) principal: SCHMIDLIN, Patrick R.
Data de Publicação: 2013
Outros Autores: MÜLLER, Phillip, ATTIN, Thomas, WIELAND, Marco, HOFER, Deborah, GUGGENHEIM, Bernhard
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/53404
Resumo: Objective To investigate the microbial adherence and colonization of a polyspecies biofilm on 7 differently processed titanium surfaces. Material and Methods Six-species biofilms were formed anaerobically on 5-mm-diameter sterilized, saliva-preconditioned titanium discs. Material surfaces used were either machined, stained, acid-etched or sandblasted/acid-etched (SLA). Samples of the latter two materials were also provided in a chemically modified form, with increased wettability characteristics. Surface roughness and contact angles of all materials were determined. The discs were then incubated anaerobically for up to 16.5 h. Initial microbial adherence was evaluated after 20 min incubation and further colonization after 2, 4, 8, and 16.5 h using non-selective and selective culture techniques. Results at different time points were compared using ANOVA and Scheffé post hoc analysis. Results The mean differences in microorganisms colonizing after the first 20 min were in a very narrow range (4.5 to 4.8 log CFU). At up to 16.5 h, the modified SLA surface exhibited the highest values for colonization (6.9±0.2 log CFU, p<0.05) but increasing growth was observed on all test surfaces over time. Discrepancies among bacterial strains on the differently crafted titanium surfaces were very similar to those described for total log CFU. F. nucleatum was below the detection limit on all surfaces after 4 h. Conclusion Within the limitations of this in vitro study, surface roughness had a moderate influence on biofilm formation, while wettability did not seem to influence biofilm formation under the experimental conditions described. The modified SLA surface showed the highest trend for bacterial colonization.
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spelling Polyspecies biofilm formation on implant surfaces with different surface characteristicsDental implantsTitaniumBiofilmsSurface propertiesWettabilityObjective To investigate the microbial adherence and colonization of a polyspecies biofilm on 7 differently processed titanium surfaces. Material and Methods Six-species biofilms were formed anaerobically on 5-mm-diameter sterilized, saliva-preconditioned titanium discs. Material surfaces used were either machined, stained, acid-etched or sandblasted/acid-etched (SLA). Samples of the latter two materials were also provided in a chemically modified form, with increased wettability characteristics. Surface roughness and contact angles of all materials were determined. The discs were then incubated anaerobically for up to 16.5 h. Initial microbial adherence was evaluated after 20 min incubation and further colonization after 2, 4, 8, and 16.5 h using non-selective and selective culture techniques. Results at different time points were compared using ANOVA and Scheffé post hoc analysis. Results The mean differences in microorganisms colonizing after the first 20 min were in a very narrow range (4.5 to 4.8 log CFU). At up to 16.5 h, the modified SLA surface exhibited the highest values for colonization (6.9±0.2 log CFU, p<0.05) but increasing growth was observed on all test surfaces over time. Discrepancies among bacterial strains on the differently crafted titanium surfaces were very similar to those described for total log CFU. F. nucleatum was below the detection limit on all surfaces after 4 h. Conclusion Within the limitations of this in vitro study, surface roughness had a moderate influence on biofilm formation, while wettability did not seem to influence biofilm formation under the experimental conditions described. The modified SLA surface showed the highest trend for bacterial colonization.Universidade de São Paulo. Faculdade de Odontologia de Bauru2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/5340410.1590/1678-7757201302312Journal of Applied Oral Science; Vol. 21 No. 1 (2013); 48-55Journal of Applied Oral Science; Vol. 21 Núm. 1 (2013); 48-55Journal of Applied Oral Science; v. 21 n. 1 (2013); 48-551678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/53404/57383Copyright (c) 2013 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessSCHMIDLIN, Patrick R.MÜLLER, PhillipATTIN, ThomasWIELAND, MarcoHOFER, DeborahGUGGENHEIM, Bernhard2014-05-08T13:21:17Zoai:revistas.usp.br:article/53404Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2014-05-08T13:21:17Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Polyspecies biofilm formation on implant surfaces with different surface characteristics
title Polyspecies biofilm formation on implant surfaces with different surface characteristics
spellingShingle Polyspecies biofilm formation on implant surfaces with different surface characteristics
SCHMIDLIN, Patrick R.
Dental implants
Titanium
Biofilms
Surface properties
Wettability
title_short Polyspecies biofilm formation on implant surfaces with different surface characteristics
title_full Polyspecies biofilm formation on implant surfaces with different surface characteristics
title_fullStr Polyspecies biofilm formation on implant surfaces with different surface characteristics
title_full_unstemmed Polyspecies biofilm formation on implant surfaces with different surface characteristics
title_sort Polyspecies biofilm formation on implant surfaces with different surface characteristics
author SCHMIDLIN, Patrick R.
author_facet SCHMIDLIN, Patrick R.
MÜLLER, Phillip
ATTIN, Thomas
WIELAND, Marco
HOFER, Deborah
GUGGENHEIM, Bernhard
author_role author
author2 MÜLLER, Phillip
ATTIN, Thomas
WIELAND, Marco
HOFER, Deborah
GUGGENHEIM, Bernhard
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv SCHMIDLIN, Patrick R.
MÜLLER, Phillip
ATTIN, Thomas
WIELAND, Marco
HOFER, Deborah
GUGGENHEIM, Bernhard
dc.subject.por.fl_str_mv Dental implants
Titanium
Biofilms
Surface properties
Wettability
topic Dental implants
Titanium
Biofilms
Surface properties
Wettability
description Objective To investigate the microbial adherence and colonization of a polyspecies biofilm on 7 differently processed titanium surfaces. Material and Methods Six-species biofilms were formed anaerobically on 5-mm-diameter sterilized, saliva-preconditioned titanium discs. Material surfaces used were either machined, stained, acid-etched or sandblasted/acid-etched (SLA). Samples of the latter two materials were also provided in a chemically modified form, with increased wettability characteristics. Surface roughness and contact angles of all materials were determined. The discs were then incubated anaerobically for up to 16.5 h. Initial microbial adherence was evaluated after 20 min incubation and further colonization after 2, 4, 8, and 16.5 h using non-selective and selective culture techniques. Results at different time points were compared using ANOVA and Scheffé post hoc analysis. Results The mean differences in microorganisms colonizing after the first 20 min were in a very narrow range (4.5 to 4.8 log CFU). At up to 16.5 h, the modified SLA surface exhibited the highest values for colonization (6.9±0.2 log CFU, p<0.05) but increasing growth was observed on all test surfaces over time. Discrepancies among bacterial strains on the differently crafted titanium surfaces were very similar to those described for total log CFU. F. nucleatum was below the detection limit on all surfaces after 4 h. Conclusion Within the limitations of this in vitro study, surface roughness had a moderate influence on biofilm formation, while wettability did not seem to influence biofilm formation under the experimental conditions described. The modified SLA surface showed the highest trend for bacterial colonization.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/53404
10.1590/1678-7757201302312
url https://www.revistas.usp.br/jaos/article/view/53404
identifier_str_mv 10.1590/1678-7757201302312
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/53404/57383
dc.rights.driver.fl_str_mv Copyright (c) 2013 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2013 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 21 No. 1 (2013); 48-55
Journal of Applied Oral Science; Vol. 21 Núm. 1 (2013); 48-55
Journal of Applied Oral Science; v. 21 n. 1 (2013); 48-55
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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