Enzymatic activity of lipase in post-metamorphic phase bullfrogs
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | https://www.revistas.usp.br/sa/article/view/22186 |
Resumo: | The knowledge of the digestive system of bullfrogs is an important step for the determination of their nutritional requirements throughout growth phases. With the objective of evaluating the enzymatic activity of lipase in the intestinal content of bullfrogs (Rana catesbeiana Shaw, 1802), 100 animals with median weight of 3.6 g were distributed in stalls under controlled temperature and photoperiod. The frogs, selected at the post-metamorphic phase, received commercial extruded diet ad libitum throughout the 87-day experiment. The collections of the intestinal content were performed by the desensitization of the frogs in ice and water at 0ºC and subsequent isolation of the small intestine. Determination of lipase activity was performed with a commercial enzymatic kit (Lipase-Bioclin, MG, Brazil), first measured in samples taken at day three (3.46 UI). During the initial phase the bullfrog possesses low lipase hydrolysis capacity was found, having a specific activity of 217 UI mg-1. In the subsequent period both lipase activity and specific lipase activity continuously increased. Lipase activity as a function of bullfrog weight fell after day twenty and reached 0.33 UI g-1, for frogs of medium weight (179 g). Feed for bullfrogs at the post-metamorphic phase weighing more than 10 g can have larger amounts of ingredients containg lipids, due to the increased digestive capacity of these frogs. |
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Enzymatic activity of lipase in post-metamorphic phase bullfrogs Atividade enzimática da lipase em rã-touro na fase pós-metamórfica atividade específicaenzima digestivaraniculturadigestive enzymefrog culturespecific activity The knowledge of the digestive system of bullfrogs is an important step for the determination of their nutritional requirements throughout growth phases. With the objective of evaluating the enzymatic activity of lipase in the intestinal content of bullfrogs (Rana catesbeiana Shaw, 1802), 100 animals with median weight of 3.6 g were distributed in stalls under controlled temperature and photoperiod. The frogs, selected at the post-metamorphic phase, received commercial extruded diet ad libitum throughout the 87-day experiment. The collections of the intestinal content were performed by the desensitization of the frogs in ice and water at 0ºC and subsequent isolation of the small intestine. Determination of lipase activity was performed with a commercial enzymatic kit (Lipase-Bioclin, MG, Brazil), first measured in samples taken at day three (3.46 UI). During the initial phase the bullfrog possesses low lipase hydrolysis capacity was found, having a specific activity of 217 UI mg-1. In the subsequent period both lipase activity and specific lipase activity continuously increased. Lipase activity as a function of bullfrog weight fell after day twenty and reached 0.33 UI g-1, for frogs of medium weight (179 g). Feed for bullfrogs at the post-metamorphic phase weighing more than 10 g can have larger amounts of ingredients containg lipids, due to the increased digestive capacity of these frogs. O conhecimento do sistema digestório da rã-touro é um passo importante para a determinação de sua exigência nutricional nas diferentes fases de crescimento. Com o objetivo de avaliar a atividade enzimática da lipase no conteúdo intestinal da rã-touro (Rana catesbeiana Shaw, 1802), 100 animais com peso médio de 3,6 g foram distribuídos em baias-teste com temperatura e fotoperíodo controlados. As rãs, selecionadas na fase pos-metamórfica receberam ração extruzada comercial ad libitum. Durante 87 dias de experimento, 29 coletas (87 rãs) foram feitas em intervalos que variaram de um a oito dias. As rãs foram insensibilizadas em água e gelo a 0ºC para subseqüente isolamento do intestino delgado e retirada do conteúdo intestinal. Para determinação da atividade da lipase foram usados conjuntos enzimáticos da BIOCLIN. A atividade da lipase foi registrada no terceiro dia (3,46 UI). Na fase inicial, a rã-touro possui baixa capacidade de hidrólise para a lipase que teve atividade específica de 217 UI mg-1. No período subseqüente, observou-se aumento contínuo da atividade e da atividade específica da lipase. A atividade da lipase em função do peso da rã-touro depois do 20º dia apresentou redução contínua, alcançando valor de 0,33 UI g-1, registrado para rãs com peso médio de 179 g. As rações para rã-touro com peso superior a 10 g podem conter quantidades maiores de ingredientes que contenham lipídos, devido ao aumento da capacidade da digestão. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/2218610.1590/S0103-90162006000500004Scientia Agricola; v. 63 n. 5 (2006); 439-443Scientia Agricola; Vol. 63 No. 5 (2006); 439-443Scientia Agricola; Vol. 63 Núm. 5 (2006); 439-4431678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/22186/24210Copyright (c) 2015 Scientia Agricolainfo:eu-repo/semantics/openAccessBraga, Luís Gustavo TavaresOliveira, Maria Goreti de AlmeidaLima, William CardosoEuclydes, Ricardo Frederico2015-07-07T17:14:38Zoai:revistas.usp.br:article/22186Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2015-07-07T17:14:38Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Enzymatic activity of lipase in post-metamorphic phase bullfrogs Atividade enzimática da lipase em rã-touro na fase pós-metamórfica |
title |
Enzymatic activity of lipase in post-metamorphic phase bullfrogs |
spellingShingle |
Enzymatic activity of lipase in post-metamorphic phase bullfrogs Braga, Luís Gustavo Tavares atividade específica enzima digestiva ranicultura digestive enzyme frog culture specific activity |
title_short |
Enzymatic activity of lipase in post-metamorphic phase bullfrogs |
title_full |
Enzymatic activity of lipase in post-metamorphic phase bullfrogs |
title_fullStr |
Enzymatic activity of lipase in post-metamorphic phase bullfrogs |
title_full_unstemmed |
Enzymatic activity of lipase in post-metamorphic phase bullfrogs |
title_sort |
Enzymatic activity of lipase in post-metamorphic phase bullfrogs |
author |
Braga, Luís Gustavo Tavares |
author_facet |
Braga, Luís Gustavo Tavares Oliveira, Maria Goreti de Almeida Lima, William Cardoso Euclydes, Ricardo Frederico |
author_role |
author |
author2 |
Oliveira, Maria Goreti de Almeida Lima, William Cardoso Euclydes, Ricardo Frederico |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Braga, Luís Gustavo Tavares Oliveira, Maria Goreti de Almeida Lima, William Cardoso Euclydes, Ricardo Frederico |
dc.subject.por.fl_str_mv |
atividade específica enzima digestiva ranicultura digestive enzyme frog culture specific activity |
topic |
atividade específica enzima digestiva ranicultura digestive enzyme frog culture specific activity |
description |
The knowledge of the digestive system of bullfrogs is an important step for the determination of their nutritional requirements throughout growth phases. With the objective of evaluating the enzymatic activity of lipase in the intestinal content of bullfrogs (Rana catesbeiana Shaw, 1802), 100 animals with median weight of 3.6 g were distributed in stalls under controlled temperature and photoperiod. The frogs, selected at the post-metamorphic phase, received commercial extruded diet ad libitum throughout the 87-day experiment. The collections of the intestinal content were performed by the desensitization of the frogs in ice and water at 0ºC and subsequent isolation of the small intestine. Determination of lipase activity was performed with a commercial enzymatic kit (Lipase-Bioclin, MG, Brazil), first measured in samples taken at day three (3.46 UI). During the initial phase the bullfrog possesses low lipase hydrolysis capacity was found, having a specific activity of 217 UI mg-1. In the subsequent period both lipase activity and specific lipase activity continuously increased. Lipase activity as a function of bullfrog weight fell after day twenty and reached 0.33 UI g-1, for frogs of medium weight (179 g). Feed for bullfrogs at the post-metamorphic phase weighing more than 10 g can have larger amounts of ingredients containg lipids, due to the increased digestive capacity of these frogs. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/22186 10.1590/S0103-90162006000500004 |
url |
https://www.revistas.usp.br/sa/article/view/22186 |
identifier_str_mv |
10.1590/S0103-90162006000500004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/22186/24210 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Scientia Agricola info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Scientia Agricola |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
dc.source.none.fl_str_mv |
Scientia Agricola; v. 63 n. 5 (2006); 439-443 Scientia Agricola; Vol. 63 No. 5 (2006); 439-443 Scientia Agricola; Vol. 63 Núm. 5 (2006); 439-443 1678-992X 0103-9016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1787713256139259904 |