Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?

Detalhes bibliográficos
Autor(a) principal: Rossi, Flavia
Data de Publicação: 2017
Outros Autores: Girardello, Raquel, Morais, Carlos, Cury, Ana Paula, Martins, Layla Farage, da Silva, Aline Maria, Abdala, Edson, Setubal, João Carlos, da Silva Duarte, Alberto José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/141021
Resumo: OBJECTIVE: We describe an IncX4 pHC891/16mcr plasmid carrying mcr-1 in a colistin-resistant and carbapenem-susceptible E. coli isolate (HC891/16), ST156, which caused a blood infection in a Brazilian patient with gallbladder adenocarcinoma. METHODS: Strain HC891/16 was subjected to whole genome sequencing using the MiSeq Platform (Illumina, Inc., USA). Assembly was performed using Mira and ABACAS. RESULTS: The isolates showed resistance only to ciprofloxacin, ampicillin and cefoxitin, and whole-genome sequencing revealed the presence of aac(6’)Ib-cr and blaTEM1. CONCLUSION: Our findings warn of the possible silent dissemination of colistin resistance by carbapenem-susceptible mcr-1 producers, as colistin susceptibility is commonly tested only among carbapenem-resistant isolates.
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spelling Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?Colistin ResistancePolymyxin ResistanceEscherichia ColiCarbapenem-SusceptibleST156OBJECTIVE: We describe an IncX4 pHC891/16mcr plasmid carrying mcr-1 in a colistin-resistant and carbapenem-susceptible E. coli isolate (HC891/16), ST156, which caused a blood infection in a Brazilian patient with gallbladder adenocarcinoma. METHODS: Strain HC891/16 was subjected to whole genome sequencing using the MiSeq Platform (Illumina, Inc., USA). Assembly was performed using Mira and ABACAS. RESULTS: The isolates showed resistance only to ciprofloxacin, ampicillin and cefoxitin, and whole-genome sequencing revealed the presence of aac(6’)Ib-cr and blaTEM1. CONCLUSION: Our findings warn of the possible silent dissemination of colistin resistance by carbapenem-susceptible mcr-1 producers, as colistin susceptibility is commonly tested only among carbapenem-resistant isolates.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2017-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/14102110.6061/clinics/2017(10)09Clinics; Vol. 72 No. 10 (2017); 642-644Clinics; v. 72 n. 10 (2017); 642-644Clinics; Vol. 72 Núm. 10 (2017); 642-6441980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/141021/136087Copyright (c) 2017 Clinicsinfo:eu-repo/semantics/openAccessRossi, FlaviaGirardello, RaquelMorais, CarlosCury, Ana PaulaMartins, Layla Farageda Silva, Aline MariaAbdala, EdsonSetubal, João Carlosda Silva Duarte, Alberto José2017-11-27T13:14:34Zoai:revistas.usp.br:article/141021Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2017-11-27T13:14:34Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
spellingShingle Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
Rossi, Flavia
Colistin Resistance
Polymyxin Resistance
Escherichia Coli
Carbapenem-Susceptible
ST156
title_short Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title_full Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title_fullStr Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title_full_unstemmed Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
title_sort Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?
author Rossi, Flavia
author_facet Rossi, Flavia
Girardello, Raquel
Morais, Carlos
Cury, Ana Paula
Martins, Layla Farage
da Silva, Aline Maria
Abdala, Edson
Setubal, João Carlos
da Silva Duarte, Alberto José
author_role author
author2 Girardello, Raquel
Morais, Carlos
Cury, Ana Paula
Martins, Layla Farage
da Silva, Aline Maria
Abdala, Edson
Setubal, João Carlos
da Silva Duarte, Alberto José
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rossi, Flavia
Girardello, Raquel
Morais, Carlos
Cury, Ana Paula
Martins, Layla Farage
da Silva, Aline Maria
Abdala, Edson
Setubal, João Carlos
da Silva Duarte, Alberto José
dc.subject.por.fl_str_mv Colistin Resistance
Polymyxin Resistance
Escherichia Coli
Carbapenem-Susceptible
ST156
topic Colistin Resistance
Polymyxin Resistance
Escherichia Coli
Carbapenem-Susceptible
ST156
description OBJECTIVE: We describe an IncX4 pHC891/16mcr plasmid carrying mcr-1 in a colistin-resistant and carbapenem-susceptible E. coli isolate (HC891/16), ST156, which caused a blood infection in a Brazilian patient with gallbladder adenocarcinoma. METHODS: Strain HC891/16 was subjected to whole genome sequencing using the MiSeq Platform (Illumina, Inc., USA). Assembly was performed using Mira and ABACAS. RESULTS: The isolates showed resistance only to ciprofloxacin, ampicillin and cefoxitin, and whole-genome sequencing revealed the presence of aac(6’)Ib-cr and blaTEM1. CONCLUSION: Our findings warn of the possible silent dissemination of colistin resistance by carbapenem-susceptible mcr-1 producers, as colistin susceptibility is commonly tested only among carbapenem-resistant isolates.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/141021
10.6061/clinics/2017(10)09
url https://www.revistas.usp.br/clinics/article/view/141021
identifier_str_mv 10.6061/clinics/2017(10)09
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/141021/136087
dc.rights.driver.fl_str_mv Copyright (c) 2017 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 72 No. 10 (2017); 642-644
Clinics; v. 72 n. 10 (2017); 642-644
Clinics; Vol. 72 Núm. 10 (2017); 642-644
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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