Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strain
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26773 |
Resumo: | Seven conventional adult horses were inoculated intranasally with a Brazilian A4/72 strain of equid herpesvirus-1 (EHV-1). In the first ten days after the inoculation, they showed signs of a mild, self- limiting upper respiratory tract infection. In spite of the presence of neutralizing antibodies before the trial, seroconversion was observed in some horses. The virus was not isolated from nasal swabs and peripheral blood leukocytes (PBL) of any of the horses. However, the EHV-1 was detected through the polymerase chain reaction (PCR) from PBL of all horses in the experiment within the third to the eighth day after the inoculation that illustrated the viremia. In addition, the PCR assay also detected the virus in bronchoalveolar lavage fluid samples starting on the ninth day after the experimental infection in most of horses. For that reason, as a diagnostic tool, the PCR assay showed higher sensitivity and specificity than the conventional laboratorial methods in detection of EHV-1. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strainDetecção do DNA do herpesvírus eqüino 1 pela reação em cadeia pela polimerase em cavalos inoculados com a estirpe brasileira A4/72CavalosHerpesvirus eqüino tipo 1DiagnósticoReação em cadeia pela polimerasePCRHorsesEquid herpesvirus-1DiagnosisPolymerase chain reactionPCRSeven conventional adult horses were inoculated intranasally with a Brazilian A4/72 strain of equid herpesvirus-1 (EHV-1). In the first ten days after the inoculation, they showed signs of a mild, self- limiting upper respiratory tract infection. In spite of the presence of neutralizing antibodies before the trial, seroconversion was observed in some horses. The virus was not isolated from nasal swabs and peripheral blood leukocytes (PBL) of any of the horses. However, the EHV-1 was detected through the polymerase chain reaction (PCR) from PBL of all horses in the experiment within the third to the eighth day after the inoculation that illustrated the viremia. In addition, the PCR assay also detected the virus in bronchoalveolar lavage fluid samples starting on the ninth day after the experimental infection in most of horses. For that reason, as a diagnostic tool, the PCR assay showed higher sensitivity and specificity than the conventional laboratorial methods in detection of EHV-1.Sete cavalos adultos de status sanitário convencional foram inoculados por via intranasal com a estirpe brasileira A4/72 do herpesvírus eqüino tipo 1 (EHV-1). Nos primeiros dez dias após a inoculação viral, todos os cavalos apresentaram manifestações de infecção respiratória leve e restrita às vias aéreas anteriores. Apesar de possuírem títulos de anticorpos neutralizantes antes da inoculação, alguns cavalos apresentaram soroconversão após o desafio viral. O EHV-1 não foi isolado a partir das secreções nasais e leucócitos sanguíneos periféricos (PBL) de nenhum animal. Entretanto, o DNA viral foi detectado pela reação em cadeia pela polimerase (PCR) nos PBL entre o terceiro e o oitavo dias pós-inoculação (d.p.i.) em todos os animais, indicando a ocorrência de viremia. Além disso, a prova de PCR detectou o vírus nas amostras do lavado broncoalveolar a partir do nono d.p.i. na maioria dos animais. Com base nos resultados obtidos, foi possível concluir que a PCR é uma técnica com alta sensibilidade e especificidade para o diagnóstico do EHV-1, capaz de detectar a presença do DNA viral mesmo quando não ocorre a constatação do agente pelos métodos tradicionais.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2009-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2677310.11606/issn.1678-4456.bjvras.2009.26773Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 Núm. 4 (2009); 256-261 Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 No. 4 (2009); 256-261 Brazilian Journal of Veterinary Research and Animal Science; v. 46 n. 4 (2009); 256-261 Brazilian Journal of Veterinary Research and Animal Science; V. 46 N. 4 (2009); 256-261 1678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/26773/28556Mori, EnioMori, Claudia Madalena CabreraMassironi, Sílvia Maria GomesCunha, Elenice Maria SequetinVillalobos, Eliana Monteforte CassaroLara, Maria do Carmo Custódio de Souza HunoldFernandes, Wilson Robertoinfo:eu-repo/semantics/openAccess2020-06-23T04:11:41Zoai:revistas.usp.br:article/26773Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:03.090551Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strain Detecção do DNA do herpesvírus eqüino 1 pela reação em cadeia pela polimerase em cavalos inoculados com a estirpe brasileira A4/72 |
title |
Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strain |
spellingShingle |
Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strain Mori, Enio Cavalos Herpesvirus eqüino tipo 1 Diagnóstico Reação em cadeia pela polimerase PCR Horses Equid herpesvirus-1 Diagnosis Polymerase chain reaction PCR |
title_short |
Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strain |
title_full |
Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strain |
title_fullStr |
Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strain |
title_full_unstemmed |
Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strain |
title_sort |
Detection of equid herpesvirus 1 DNA by polymerase chain reaction after experimental inoculation of horses with a brazilian A4/72 strain |
author |
Mori, Enio |
author_facet |
Mori, Enio Mori, Claudia Madalena Cabrera Massironi, Sílvia Maria Gomes Cunha, Elenice Maria Sequetin Villalobos, Eliana Monteforte Cassaro Lara, Maria do Carmo Custódio de Souza Hunold Fernandes, Wilson Roberto |
author_role |
author |
author2 |
Mori, Claudia Madalena Cabrera Massironi, Sílvia Maria Gomes Cunha, Elenice Maria Sequetin Villalobos, Eliana Monteforte Cassaro Lara, Maria do Carmo Custódio de Souza Hunold Fernandes, Wilson Roberto |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Mori, Enio Mori, Claudia Madalena Cabrera Massironi, Sílvia Maria Gomes Cunha, Elenice Maria Sequetin Villalobos, Eliana Monteforte Cassaro Lara, Maria do Carmo Custódio de Souza Hunold Fernandes, Wilson Roberto |
dc.subject.por.fl_str_mv |
Cavalos Herpesvirus eqüino tipo 1 Diagnóstico Reação em cadeia pela polimerase PCR Horses Equid herpesvirus-1 Diagnosis Polymerase chain reaction PCR |
topic |
Cavalos Herpesvirus eqüino tipo 1 Diagnóstico Reação em cadeia pela polimerase PCR Horses Equid herpesvirus-1 Diagnosis Polymerase chain reaction PCR |
description |
Seven conventional adult horses were inoculated intranasally with a Brazilian A4/72 strain of equid herpesvirus-1 (EHV-1). In the first ten days after the inoculation, they showed signs of a mild, self- limiting upper respiratory tract infection. In spite of the presence of neutralizing antibodies before the trial, seroconversion was observed in some horses. The virus was not isolated from nasal swabs and peripheral blood leukocytes (PBL) of any of the horses. However, the EHV-1 was detected through the polymerase chain reaction (PCR) from PBL of all horses in the experiment within the third to the eighth day after the inoculation that illustrated the viremia. In addition, the PCR assay also detected the virus in bronchoalveolar lavage fluid samples starting on the ninth day after the experimental infection in most of horses. For that reason, as a diagnostic tool, the PCR assay showed higher sensitivity and specificity than the conventional laboratorial methods in detection of EHV-1. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26773 10.11606/issn.1678-4456.bjvras.2009.26773 |
url |
https://www.revistas.usp.br/bjvras/article/view/26773 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2009.26773 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26773/28556 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 Núm. 4 (2009); 256-261 Brazilian Journal of Veterinary Research and Animal Science; Vol. 46 No. 4 (2009); 256-261 Brazilian Journal of Veterinary Research and Animal Science; v. 46 n. 4 (2009); 256-261 Brazilian Journal of Veterinary Research and Animal Science; V. 46 N. 4 (2009); 256-261 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051559355351040 |