Efeitos do tratamento com nanopartículas de ouro na adiposidade, resposta inflamatória e parâmetros bioquímicos em camundongos submetidos a obesidade

• Autor(a) principal: Prá, Morgana
• Data de Publicação: 2018
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Universitário da Ânima (RUNA)
• Texto Completo: https://repositorio.animaeducacao.com.br/handle/ANIMA/3146
• topic: Obesidade; Inflamação; Estresse oxidativo; Nanotecnologia; Nanopartículas de ouro

Introduction: Obesity is a worldwide epidemic and its pathophysiology is related to inflammation, oxidative stress and mitochondrial dysfunction. Due to the lack of treatments, there is a need for new therapeutic options. Thus, gold nanoparticles with anti-inflammatory properties could be an alternative for the treatment of this disease. Objective: To evaluate the effects of gold nanoparticle treatment on adiposity, inflammatory response and biochemical parameters in mice submitted to obesity. Methods: It was synthesized and characterized gold nanoparticles of 18nm, isolated or associated with soy lecithin, administered intraperitoneally in mice once daily, single administration or for 14 days. Twenty-four hours after the last administration the animals were killed, tissues removed for evaluation of gold and serum nanoparticle concentration for hepatic and renal toxicity evaluation. After this step, the mice were submitted to obesity through a normolipid diet (control) or hyperlipid diet (obese), and treated with saline or gold nanoparticles after 8 weeks, once a day for 14 days, until completing 10 weeks of experiment. At the end, the animals were killed, serum was collected for glucose and lipid profile analysis, and the visceral fat and brain were removed for inflammatory and biochemical analysis. Results: A higher concentration of gold nanoparticles was found in the tissues when administered alone, and there was no hepatic or renal damage. In obese mice, administration of gold nanoparticles did not reduce body weight and visceral fat, but reduced food intake. Glucose levels were reversed, but the lipid profile was not altered in any of the groups. The gold nanoparticles presented a beneficial effect on the parameters of inflammation and oxidative stress, without reversing mitochondrial dysfunction. Conclusion: The use of gold nanoparticles was positive for reducing food consumption, inflammatory conditions and oxidative stress, and provides as a possible promise in the treatment of obesity.