Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupuntura

Detalhes bibliográficos
Autor(a) principal: Caron, Cintia Vieira
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Universitário da Ânima (RUNA)
Texto Completo: https://repositorio.animaeducacao.com.br/handle/ANIMA/3052
Resumo: Although the effects of electroacupuncture (EA) on pain and inflammation are similar to the activation effects of formyl peptide receptor 2 (FPR2/ALXR) mediated by pro-resolving substances, the involvement of this receptor in the antihyperalgesic effect of EA had not yet been investigated. Objective: To evaluate the involvement of the peripheral and spinal FPR2/ALXR receptor in the antihyperalgesic effect induced by low-frequency EA in an animal model of persistent peripheral inflammation. Methods: Swiss male mice (30 to 40 g) underwent intraplantar (i.pl.) injection with complete Freund's adjuvant (CFA). Mechanical hyperalgesia was assessed by the von Frey test. The animals were treated with EA (in SP6 and ST36 acupoints) or BML-111 (an analog of lipoxin A4 [LXA4]) for 5 consecutive days. In the analysis of the mechanism of action the mice were treated on the first and fifth day. The edema was evaluated on the fourth day after CFA by measuring the thickness of the paw. Intraplantar (i.pl.) and intrathecal (i.t.) administration of the FPR2/ALXR receptor antagonist (WRW4) or naloxone were performed on the first and fifth day after CFA. Expression of the FPR2/ALXR receptor in the paw and spinal cord was performed on the second day after CFA by the Western Blotting technique. Results: EA and BML-111 reduced mechanical hyperalgesia. Daily treatment for 4 consecutive days with EA or BML-111 did not reduce paw edema. The i.pl. or i.t. pre-treatment of animals with WRW4 or naloxone prevented the antihyperalgesic effect induced by EA or BML-111. The CFA i.pl. injection increased FPR2/ALXR receptor expression in the paw, but not in the spinal cord. Treatment of animals with EA or BML-111 did not alter FPR2/ALXR receptor expression in the paw or spinal cord, although animals treated with EA or BML-111 when pretreated with WRW4 had lower FPR2/ALXR receptor expression in the spinal cord. However, lower expression of the FPR2/ALXR receptor in the paw was evidenced only in animals pretreated with WRW4 that received EA treatment. Conclusion: Treatment with EA or BML-111 reduced mechanical hyperalgesia, but not edema, and this effect might be mediated by activation of peripheral and central FPR2/ALXR receptor.
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spelling Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupunturaThe role of peripheral and spinal FPR2/ALXR receptor in electroacupuncture-induced analgesia in an animal model of persistent inflammatory painInflamação crônicaMedicina integrativaMediadores pró-resolutivosAlthough the effects of electroacupuncture (EA) on pain and inflammation are similar to the activation effects of formyl peptide receptor 2 (FPR2/ALXR) mediated by pro-resolving substances, the involvement of this receptor in the antihyperalgesic effect of EA had not yet been investigated. Objective: To evaluate the involvement of the peripheral and spinal FPR2/ALXR receptor in the antihyperalgesic effect induced by low-frequency EA in an animal model of persistent peripheral inflammation. Methods: Swiss male mice (30 to 40 g) underwent intraplantar (i.pl.) injection with complete Freund's adjuvant (CFA). Mechanical hyperalgesia was assessed by the von Frey test. The animals were treated with EA (in SP6 and ST36 acupoints) or BML-111 (an analog of lipoxin A4 [LXA4]) for 5 consecutive days. In the analysis of the mechanism of action the mice were treated on the first and fifth day. The edema was evaluated on the fourth day after CFA by measuring the thickness of the paw. Intraplantar (i.pl.) and intrathecal (i.t.) administration of the FPR2/ALXR receptor antagonist (WRW4) or naloxone were performed on the first and fifth day after CFA. Expression of the FPR2/ALXR receptor in the paw and spinal cord was performed on the second day after CFA by the Western Blotting technique. Results: EA and BML-111 reduced mechanical hyperalgesia. Daily treatment for 4 consecutive days with EA or BML-111 did not reduce paw edema. The i.pl. or i.t. pre-treatment of animals with WRW4 or naloxone prevented the antihyperalgesic effect induced by EA or BML-111. The CFA i.pl. injection increased FPR2/ALXR receptor expression in the paw, but not in the spinal cord. Treatment of animals with EA or BML-111 did not alter FPR2/ALXR receptor expression in the paw or spinal cord, although animals treated with EA or BML-111 when pretreated with WRW4 had lower FPR2/ALXR receptor expression in the spinal cord. However, lower expression of the FPR2/ALXR receptor in the paw was evidenced only in animals pretreated with WRW4 that received EA treatment. Conclusion: Treatment with EA or BML-111 reduced mechanical hyperalgesia, but not edema, and this effect might be mediated by activation of peripheral and central FPR2/ALXR receptor.Apesar dos efeitos analgésicos da eletroacupuntura (EA) na dor e inflamação serem semelhantes aos efeitos da ativação do receptor 2 para peptídeos formilados (FPR2/ALX) mediada por substâncias pró-resolutivas, o envolvimento deste receptor no efeito anti-hiperalgésico da EA ainda não tinha sido investigado. Objetivo: Avaliar o envolvimento do receptor FPR2/ALX periférico e espinal no efeito anti-hiperalgésico induzido pela EA de baixa frequência em um modelo animal de inflamação periférica persistente. Métodos: Camundongos machos Suíços (30 a 40 g) foram submetidos a injeção intraplantar (i.pl.) de 20 μl adjuvante complete de Freund (CFA). A hiperalgesia mecânica foi avaliada pelo teste de von Frey. Os animais foram tratados com EA (nos acupontos SP6 e ST36) ou BML-111 nas doses 100 µl / animal: testados 0,3, 1 e 3 μg (um análogo da lipoxina A4 [LXA4]) por 5 dias consecutivos. Na análise do mecanismo de ação os camundongos foram tratados no primeiro e no quinto dia. O edema foi avaliado no quarto dia após CFA por meio da mensuração da espessura da pata. Administração i.pl. e intratecal (i.t.) do antagonista para o receptor FPR2/ALX (WRW4 nas doses de 3 ou 10 μg/i.pl) ou de naloxona foram realizadas no primeiro e quinto dia após CFA. A expressão do receptor FPR2/ALX na pata e na medula espinal foi realizada no segundo dia após CFA por meio da técnica de Western Blotting. Resultados: EA e BML-111 reduziram a hiperalgesia mecânica. O tratamento diário por 4 dias consecutivos com EA ou BML-111 não reduziram o edema de pata. O pré-tratamento i.pl. ou i.t. dos animais com WRW4 ou naloxona preveniram o efeito anti-hiperalgésico induzido pela EA ou BML-111. A injeção de CFA aumentou a expressão do receptor FPR2/ALX na pata, mas não na medula espinal. O tratamento dos animais com EA ou BML-111 não alterou a expressão do receptor FPR2/ALX na pata ou medula espinal, embora os animais tratados com EA ou BML-111 quando pré-tratados com WRW4 apresentaram menor expressão do receptor FPR2/ALX na medula espinal. No entanto, menor expressão do receptor FPR2/ALX na pata foi evidenciada somente nos animais pré-tratados com WRW4 que receberam EA. Conclusão: O tratamento com EA ou BML-111 reduziram a hiperalgesia mecânica, mas não o edema, e este efeito pode ser dependente da ativação do receptor FPR2/ALX periférico e central.Martins, Daniel FernandesCaron, Cintia Vieira2018-09-19T10:59:57Z2020-11-26T20:52:55Z2018-09-19T10:59:57Z2020-11-26T20:52:55Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis101 f.application/pdfhttps://repositorio.animaeducacao.com.br/handle/ANIMA/3052Programa de Pós-Graduação em Ciência da SaúdePalhoça/ SCAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessporreponame:Repositório Universitário da Ânima (RUNA)instname:Ânima Educaçãoinstacron:Ânima2020-12-01T16:47:17Zoai:repositorio.animaeducacao.com.br:ANIMA/3052Repositório InstitucionalPRIhttps://repositorio.animaeducacao.com.br/oai/requestcontato@animaeducacao.com.bropendoar:2020-12-01T16:47:17Repositório Universitário da Ânima (RUNA) - Ânima Educaçãofalse
dc.title.none.fl_str_mv Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupuntura
The role of peripheral and spinal FPR2/ALXR receptor in electroacupuncture-induced analgesia in an animal model of persistent inflammatory pain
title Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupuntura
spellingShingle Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupuntura
Caron, Cintia Vieira
Inflamação crônica
Medicina integrativa
Mediadores pró-resolutivos
title_short Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupuntura
title_full Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupuntura
title_fullStr Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupuntura
title_full_unstemmed Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupuntura
title_sort Avaliação do envolvimento do receptor FPR2/ALXR periférico e espinal no efeito anti-hiperalgésico induzido pela eletroacupuntura
author Caron, Cintia Vieira
author_facet Caron, Cintia Vieira
author_role author
dc.contributor.none.fl_str_mv Martins, Daniel Fernandes
dc.contributor.author.fl_str_mv Caron, Cintia Vieira
dc.subject.por.fl_str_mv Inflamação crônica
Medicina integrativa
Mediadores pró-resolutivos
topic Inflamação crônica
Medicina integrativa
Mediadores pró-resolutivos
description Although the effects of electroacupuncture (EA) on pain and inflammation are similar to the activation effects of formyl peptide receptor 2 (FPR2/ALXR) mediated by pro-resolving substances, the involvement of this receptor in the antihyperalgesic effect of EA had not yet been investigated. Objective: To evaluate the involvement of the peripheral and spinal FPR2/ALXR receptor in the antihyperalgesic effect induced by low-frequency EA in an animal model of persistent peripheral inflammation. Methods: Swiss male mice (30 to 40 g) underwent intraplantar (i.pl.) injection with complete Freund's adjuvant (CFA). Mechanical hyperalgesia was assessed by the von Frey test. The animals were treated with EA (in SP6 and ST36 acupoints) or BML-111 (an analog of lipoxin A4 [LXA4]) for 5 consecutive days. In the analysis of the mechanism of action the mice were treated on the first and fifth day. The edema was evaluated on the fourth day after CFA by measuring the thickness of the paw. Intraplantar (i.pl.) and intrathecal (i.t.) administration of the FPR2/ALXR receptor antagonist (WRW4) or naloxone were performed on the first and fifth day after CFA. Expression of the FPR2/ALXR receptor in the paw and spinal cord was performed on the second day after CFA by the Western Blotting technique. Results: EA and BML-111 reduced mechanical hyperalgesia. Daily treatment for 4 consecutive days with EA or BML-111 did not reduce paw edema. The i.pl. or i.t. pre-treatment of animals with WRW4 or naloxone prevented the antihyperalgesic effect induced by EA or BML-111. The CFA i.pl. injection increased FPR2/ALXR receptor expression in the paw, but not in the spinal cord. Treatment of animals with EA or BML-111 did not alter FPR2/ALXR receptor expression in the paw or spinal cord, although animals treated with EA or BML-111 when pretreated with WRW4 had lower FPR2/ALXR receptor expression in the spinal cord. However, lower expression of the FPR2/ALXR receptor in the paw was evidenced only in animals pretreated with WRW4 that received EA treatment. Conclusion: Treatment with EA or BML-111 reduced mechanical hyperalgesia, but not edema, and this effect might be mediated by activation of peripheral and central FPR2/ALXR receptor.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-19T10:59:57Z
2018-09-19T10:59:57Z
2018
2020-11-26T20:52:55Z
2020-11-26T20:52:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
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url https://repositorio.animaeducacao.com.br/handle/ANIMA/3052
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Programa de Pós-Graduação em Ciência da Saúde
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 101 f.
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