Efeitos do extrato hidroalcoólico de Polygala Paniculata l. sobre a regeneração funcional e morfológica em um modelo animal de lesão compressiva do nervo isquiático

• Autor(a) principal: Batisti, Ana Paula
• Data de Publicação: 2018
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Universitário da Ânima (RUNA)
• Texto Completo: https://repositorio.animaeducacao.com.br/handle/ANIMA/3125
• topic: Dor neuropática; Medicina Integrativa; Polygala paniculata L

Introduction: Neuropathies are very common injuries, comorbidities associated and very hard to treat. The search of new therapies for promote pain control and improve nerve regeneration is a challenge for researchers. Among several plants with therapeutic potential, Polygala paniculata L is a target of studies. It is native from Brazilian atlantic coast and stands out for its compounds and popular effects in inflammation and rheumatic diseases. Objective: Evaluate the Polygala paniculata L hydroalcoholic extract (PPHE) treatment effect on mice subjected sciatic nerve traumatic injury. Methods: Swiss male mice (25 to 35g) were submitted to sciatic nerve crush injury (INC). Mechanical hyperalgesia was evaluated by von Frey test (response frequency) and cold hyperalgesia by acetone drop method for 21 days after injury. Motor functional recovery was assessed through sciatic functional index and sciatic static index. The animals were treated (or not) with 0.1, 1 or 10 mg/kg doses PO. Histomorphometric analysis of sciatic nerve were conducted to assess nerve regeneration 21 days after crush injury. Cytokines concentrations in sciatic nerve and spinal cord lumbar segments were measured through ELISA twenty-one days after nerve injury. Results: All PPHE doses reduced mechanical and cold hyperalgesia with accumulative effect reaching 12 hours after PPHE administration. The treatment accelerated the motor functional recovery of mice. Besides that, the highest treatment dose group (10 mg/kg) presented greater myelinated tissue area and myelin sheath thickness. Furthermore, could reduce IL1β and TNF concentrations in sciatic nerve. However, 0.1 mg/kg group only could improve IL-10 nerve concentration. At the spinal cord, all PPHE doses reduced these pro-inflammatory cytokines concentrations. Conclusion: The treatment with PPHE was effective reducing hyperalgesia and promoting accelerated peripheral nerve regeneration in mice after sciatic nerve crush injury.