Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação

• Autor(a) principal: Belmonte, Luiz Augusto Oliveira
• Data de Publicação: 2018
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Universitário da Ânima (RUNA)
• Texto Completo: https://repositorio.animaeducacao.com.br/handle/ANIMA/3093
• topic: DPIC; Exercício físico; Hiperalgesia mecânica; Exercício intervalado

Introduction: Physical exercise is an important and adjuvant tool in the treatment of chronic pain. It is necessary to understand the analgesic effects in different prescriptions and their repercussions in the central nervous system. Objective: To evaluate the effects of continuous and interval running on treadmill on mechanical hyperalgesia in an animal model of chronic post-ischemia pain (CPIP), as well as to analyze the mechanism of action of this effect. Methods: Different groups of male Swiss mice with CPIP, induced by 3 hours of paw ischemia followed by reperfusion (IR), ran on the treadmill in different protocols, the speed (10, 13, 16 or 19 m/min), duration (15, 30 or 60 minutes), weekly frequency (3 or 5 times), weekly increase in continuous and interval running speed, were tested. Mechanical hyperalgesia was evaluated by von Frey filament at 7, 14 and 21 days after paw IR. On the 11th day after paw IR and after 5 days of continuous and interval running, concentrations of TNF-α, IL-1β, IL-6, IL-10, lipid peroxidation, carbonylated proteins, activity of the enzymes superoxide dismutase and catalase in the prefrontal cortex, brain stem and spinal cord were performed. In addition, quantification of ERK 1/2 and AKT 1/2/3 expression in the spinal cord was performed. Involvement of opioid receptors was investigated by administration of the opioid antagonist (naloxone) on the 7th day after paw IR. Results: The results showed that continuous running has antihyperalgesic effect dependent on intensity and volume. Interval running has a longer lasting antihyperalgesic effect (up to 5 hours) than continuous running. The antihyperalgesic effect is dependent on intensity and volume in continuous running and increasing speed mantains the antihyperalgesic effect in both protocols. Both running protocols were in the moderate domain. In the pre-frontal cortex only, the interval running decreased TNF-α and IL-6 and increased IL-10, both increased SOD. In the brainstem and spinal cord both runs decreased TNF-α and IL-6 levels and increased IL-10. Both running protocols reduced oxidative damage in the spinal cord. Only the interval running had lower concentrations of phosphorylated ERK 1/2 in the spinal cord. The opioid system participates in the antihyperalgesic effect induced by continuous running, but not in the interval running. Conclusion: Interval running presented a great antihyperalgesic potential with more promising results than continuous running, which may be due to the fact that the interval running can activate different mechanisms from those activated by continuous running. Thus, interval running appears as an important alternative for the prescription of adjuvant treatments in other animal models and/or in patients with chronic pain.