Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação

Detalhes bibliográficos
Autor(a) principal: Belmonte, Luiz Augusto Oliveira
Data de Publicação: 2018
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Universitário da Ânima (RUNA)
Texto Completo: https://repositorio.animaeducacao.com.br/handle/ANIMA/3093
Resumo: Introduction: Physical exercise is an important and adjuvant tool in the treatment of chronic pain. It is necessary to understand the analgesic effects in different prescriptions and their repercussions in the central nervous system. Objective: To evaluate the effects of continuous and interval running on treadmill on mechanical hyperalgesia in an animal model of chronic post-ischemia pain (CPIP), as well as to analyze the mechanism of action of this effect. Methods: Different groups of male Swiss mice with CPIP, induced by 3 hours of paw ischemia followed by reperfusion (IR), ran on the treadmill in different protocols, the speed (10, 13, 16 or 19 m/min), duration (15, 30 or 60 minutes), weekly frequency (3 or 5 times), weekly increase in continuous and interval running speed, were tested. Mechanical hyperalgesia was evaluated by von Frey filament at 7, 14 and 21 days after paw IR. On the 11th day after paw IR and after 5 days of continuous and interval running, concentrations of TNF-α, IL-1β, IL-6, IL-10, lipid peroxidation, carbonylated proteins, activity of the enzymes superoxide dismutase and catalase in the prefrontal cortex, brain stem and spinal cord were performed. In addition, quantification of ERK 1/2 and AKT 1/2/3 expression in the spinal cord was performed. Involvement of opioid receptors was investigated by administration of the opioid antagonist (naloxone) on the 7th day after paw IR. Results: The results showed that continuous running has antihyperalgesic effect dependent on intensity and volume. Interval running has a longer lasting antihyperalgesic effect (up to 5 hours) than continuous running. The antihyperalgesic effect is dependent on intensity and volume in continuous running and increasing speed mantains the antihyperalgesic effect in both protocols. Both running protocols were in the moderate domain. In the pre-frontal cortex only, the interval running decreased TNF-α and IL-6 and increased IL-10, both increased SOD. In the brainstem and spinal cord both runs decreased TNF-α and IL-6 levels and increased IL-10. Both running protocols reduced oxidative damage in the spinal cord. Only the interval running had lower concentrations of phosphorylated ERK 1/2 in the spinal cord. The opioid system participates in the antihyperalgesic effect induced by continuous running, but not in the interval running. Conclusion: Interval running presented a great antihyperalgesic potential with more promising results than continuous running, which may be due to the fact that the interval running can activate different mechanisms from those activated by continuous running. Thus, interval running appears as an important alternative for the prescription of adjuvant treatments in other animal models and/or in patients with chronic pain.
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spelling Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de açãoThe effects of continuous and interval running on treadmill in paw in mice of chronic post-ischemia pain (CPIP): analyze the mechanism of actionDPICExercício físicoHiperalgesia mecânicaExercício intervaladoIntroduction: Physical exercise is an important and adjuvant tool in the treatment of chronic pain. It is necessary to understand the analgesic effects in different prescriptions and their repercussions in the central nervous system. Objective: To evaluate the effects of continuous and interval running on treadmill on mechanical hyperalgesia in an animal model of chronic post-ischemia pain (CPIP), as well as to analyze the mechanism of action of this effect. Methods: Different groups of male Swiss mice with CPIP, induced by 3 hours of paw ischemia followed by reperfusion (IR), ran on the treadmill in different protocols, the speed (10, 13, 16 or 19 m/min), duration (15, 30 or 60 minutes), weekly frequency (3 or 5 times), weekly increase in continuous and interval running speed, were tested. Mechanical hyperalgesia was evaluated by von Frey filament at 7, 14 and 21 days after paw IR. On the 11th day after paw IR and after 5 days of continuous and interval running, concentrations of TNF-α, IL-1β, IL-6, IL-10, lipid peroxidation, carbonylated proteins, activity of the enzymes superoxide dismutase and catalase in the prefrontal cortex, brain stem and spinal cord were performed. In addition, quantification of ERK 1/2 and AKT 1/2/3 expression in the spinal cord was performed. Involvement of opioid receptors was investigated by administration of the opioid antagonist (naloxone) on the 7th day after paw IR. Results: The results showed that continuous running has antihyperalgesic effect dependent on intensity and volume. Interval running has a longer lasting antihyperalgesic effect (up to 5 hours) than continuous running. The antihyperalgesic effect is dependent on intensity and volume in continuous running and increasing speed mantains the antihyperalgesic effect in both protocols. Both running protocols were in the moderate domain. In the pre-frontal cortex only, the interval running decreased TNF-α and IL-6 and increased IL-10, both increased SOD. In the brainstem and spinal cord both runs decreased TNF-α and IL-6 levels and increased IL-10. Both running protocols reduced oxidative damage in the spinal cord. Only the interval running had lower concentrations of phosphorylated ERK 1/2 in the spinal cord. The opioid system participates in the antihyperalgesic effect induced by continuous running, but not in the interval running. Conclusion: Interval running presented a great antihyperalgesic potential with more promising results than continuous running, which may be due to the fact that the interval running can activate different mechanisms from those activated by continuous running. Thus, interval running appears as an important alternative for the prescription of adjuvant treatments in other animal models and/or in patients with chronic pain.Introdução: O exercício físico é uma ferramenta importante e adjuvante no tratamento da dor crônica. É necessária a compreensão dos efeitos analgésicos em diferentes prescrições e suas repercussões no sistema nervoso central. Objetivo: Avaliar os efeitos da corrida contínua e intervalada em esteira sobre a hiperalgesia mecânica em um modelo animal de dor pós isquemia crônica (DPIC), bem como analisar o mecanismo de ação deste efeito. Métodos: Diferentes grupos de camundongos Suíços machos com DPIC, induzida por 3 horas de isquemia da pata seguida por reperfusão (IR), correram na esteira em diferentes protocolos testando-se a velocidade (10, 13, 16 ou 19 m/min), duração (15, 30 ou 60 minutos), frequência semanal (3 ou 5 vezes), aumento semanal da velocidade da corrida contínua e intervalada. A hiperalgesia mecânica foi avaliada pelo filamento de von Frey em 7, 14 e 21 dias após IR da pata. No 11º dia após IR da pata e após 5 dias de corrida contínua e intervalada, dosagens das concentrações de TNF-α, IL-1β, IL-6, IL-10, peroxidação lipídica, proteínas carboniladas, atividade das enzimas superóxido dismutase e catalase no córtex préfrontal, tronco encefálico e medula espinal foram realizadas. Além disso, quantificação da expressão de ERK 1/2 e AKT 1/2/3 na medula espinal foi realizada. O envolvimento dos receptores opioides foi investigado pela administração do antagonista (naloxona) opioide no 7º dia após IR da pata. Resultados: Os resultados demonstraram que a corrida contínua apresenta efeito anti-hiperalgésico dependente da intensidade e volume. A corrida intervalada apresenta efeito anti-hiperalgésico mais duradouro (por até 5 horas) que a corrida contínua. O efeito anti-hiperalgésico é dependente da intensidade e do volume na corrida contínua e o aumento da velocidade mantém o efeito anti-hiperalgésico em ambos protocolos. Os dois protocolos de corrida se encontraram no domínio moderado. No córtex pré-frontal somente a corrida intervalada diminui TNF-α e IL-6 e aumentou IL-10 e ambas aumentaram a SOD. No tronco encefálico e medula espinal ambas corridas diminuíram as concentrações de TNF-α e IL-6 e aumentaram IL-10. Ambos protocolos de corrida reduziram o dano oxidativo na medula espinal. Somente a corrida intervalada apresentou concentrações menores de ERK1/2 fosforilada na medula espinal. O sistema opioide participa do efeito anti-hiperalgésico induzida pela corrida contínua, mas não na intervalada. Conclusão: A corrida intervalada apresentou grande potencial anti-hiperalgésico com resultados mais promissores do que a corrida contínua, o que pode estar ao fato da corrida intervalada ativar diferentes mecanismos daqueles ativados pela corrida continua. Assim, a corrida intervalada surge como uma alternativa importante para a prescrição de tratamentos adjuvantes em outros modelos animais e/ou em pacientes com dores crônicas.Martins, Daniel FernandesBelmonte, Luiz Augusto Oliveira2019-11-11T11:14:44Z2020-11-26T20:58:11Z2019-11-11T11:14:44Z2020-11-26T20:58:11Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis129 f.application/pdfhttps://repositorio.animaeducacao.com.br/handle/ANIMA/3093Programa de Pós-Graduação em Ciência da SaúdePalhoça -SCAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessporreponame:Repositório Universitário da Ânima (RUNA)instname:Ânima Educaçãoinstacron:Ânima2020-12-01T16:48:10Zoai:repositorio.animaeducacao.com.br:ANIMA/3093Repositório InstitucionalPRIhttps://repositorio.animaeducacao.com.br/oai/requestcontato@animaeducacao.com.bropendoar:2020-12-01T16:48:10Repositório Universitário da Ânima (RUNA) - Ânima Educaçãofalse
dc.title.none.fl_str_mv Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação
The effects of continuous and interval running on treadmill in paw in mice of chronic post-ischemia pain (CPIP): analyze the mechanism of action
title Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação
spellingShingle Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação
Belmonte, Luiz Augusto Oliveira
DPIC
Exercício físico
Hiperalgesia mecânica
Exercício intervalado
title_short Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação
title_full Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação
title_fullStr Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação
title_full_unstemmed Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação
title_sort Os efeitos da corrida contínua versus intervalada na dor pós isquemia crônica da pata em camundongos: análise do mecanismo de ação
author Belmonte, Luiz Augusto Oliveira
author_facet Belmonte, Luiz Augusto Oliveira
author_role author
dc.contributor.none.fl_str_mv Martins, Daniel Fernandes
dc.contributor.author.fl_str_mv Belmonte, Luiz Augusto Oliveira
dc.subject.por.fl_str_mv DPIC
Exercício físico
Hiperalgesia mecânica
Exercício intervalado
topic DPIC
Exercício físico
Hiperalgesia mecânica
Exercício intervalado
description Introduction: Physical exercise is an important and adjuvant tool in the treatment of chronic pain. It is necessary to understand the analgesic effects in different prescriptions and their repercussions in the central nervous system. Objective: To evaluate the effects of continuous and interval running on treadmill on mechanical hyperalgesia in an animal model of chronic post-ischemia pain (CPIP), as well as to analyze the mechanism of action of this effect. Methods: Different groups of male Swiss mice with CPIP, induced by 3 hours of paw ischemia followed by reperfusion (IR), ran on the treadmill in different protocols, the speed (10, 13, 16 or 19 m/min), duration (15, 30 or 60 minutes), weekly frequency (3 or 5 times), weekly increase in continuous and interval running speed, were tested. Mechanical hyperalgesia was evaluated by von Frey filament at 7, 14 and 21 days after paw IR. On the 11th day after paw IR and after 5 days of continuous and interval running, concentrations of TNF-α, IL-1β, IL-6, IL-10, lipid peroxidation, carbonylated proteins, activity of the enzymes superoxide dismutase and catalase in the prefrontal cortex, brain stem and spinal cord were performed. In addition, quantification of ERK 1/2 and AKT 1/2/3 expression in the spinal cord was performed. Involvement of opioid receptors was investigated by administration of the opioid antagonist (naloxone) on the 7th day after paw IR. Results: The results showed that continuous running has antihyperalgesic effect dependent on intensity and volume. Interval running has a longer lasting antihyperalgesic effect (up to 5 hours) than continuous running. The antihyperalgesic effect is dependent on intensity and volume in continuous running and increasing speed mantains the antihyperalgesic effect in both protocols. Both running protocols were in the moderate domain. In the pre-frontal cortex only, the interval running decreased TNF-α and IL-6 and increased IL-10, both increased SOD. In the brainstem and spinal cord both runs decreased TNF-α and IL-6 levels and increased IL-10. Both running protocols reduced oxidative damage in the spinal cord. Only the interval running had lower concentrations of phosphorylated ERK 1/2 in the spinal cord. The opioid system participates in the antihyperalgesic effect induced by continuous running, but not in the interval running. Conclusion: Interval running presented a great antihyperalgesic potential with more promising results than continuous running, which may be due to the fact that the interval running can activate different mechanisms from those activated by continuous running. Thus, interval running appears as an important alternative for the prescription of adjuvant treatments in other animal models and/or in patients with chronic pain.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-11-11T11:14:44Z
2019-11-11T11:14:44Z
2020-11-26T20:58:11Z
2020-11-26T20:58:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.animaeducacao.com.br/handle/ANIMA/3093
url https://repositorio.animaeducacao.com.br/handle/ANIMA/3093
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Programa de Pós-Graduação em Ciência da Saúde
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 129 f.
application/pdf
dc.coverage.none.fl_str_mv Palhoça -SC
dc.source.none.fl_str_mv reponame:Repositório Universitário da Ânima (RUNA)
instname:Ânima Educação
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reponame_str Repositório Universitário da Ânima (RUNA)
collection Repositório Universitário da Ânima (RUNA)
repository.name.fl_str_mv Repositório Universitário da Ânima (RUNA) - Ânima Educação
repository.mail.fl_str_mv contato@animaeducacao.com.br
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