A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice

Detalhes bibliográficos
Autor(a) principal: Reitz, Felipe
Data de Publicação: 2018
Outros Autores: Salm, Daiana C, Ludtcke, Daniela Dero, Santos, Aureo dos, Traebert, Jefferson, Martins, Daniel Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Universitário da Ânima (RUNA)
Texto Completo: https://repositorio.animaeducacao.com.br/handle/ANIMA/2679
Resumo: Background: Preclinical studies measure withdrawal responses to evoking thermal and mechanical stimuli instead of the more clinically important spontaneous pain. New method: Therefore, we studied the effect of peripheral inflammation induced by intraplantar administration of complete Freund’s adjuvant (CFA) in mice on the variability of temperature and bioimpedance as an index of pain produced by inflammation. To this end, we initially determined mathematical scores based on changes in temperature and bioimpedance (STB) for animals with an inflamed paw and compared these scores with commonly used measures of inflammatory pain. We then pharmacologically validated the tool using dexamethasone. Results: The STB analysis resembled the response found in the von Frey Hair (vFH) test. The CFA-induced increase in STB and vFH tests were reversed by intraperitoneal administration of dexamethasone. The correlation between the STB and vFH measurements showed a high correlation coefficient (R2=0.911, p < 0.001). Comparison with existing method: Our results also demonstrated that CFA paw injection induced mechanical hyperalgesia in mice and remained virtually unaltered during all time-points tested for 5 days, as measured with vFHs. The administration of CFA into the paw induced a large increase in paw volume that was apparent 1 and 5 days after the injection. The CFA injection resulted in a significant (p<0.05) decrease in the response latency to the heat stimulus, as evaluated on day 4 post-CFA injection. Conclusions: The data presented here suggest that STB may provide a novel non-invasive approach for inflammatory pain detection.
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spelling A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in miceBehavioral testInflammatory painPain measurementsThermographyBackground: Preclinical studies measure withdrawal responses to evoking thermal and mechanical stimuli instead of the more clinically important spontaneous pain. New method: Therefore, we studied the effect of peripheral inflammation induced by intraplantar administration of complete Freund’s adjuvant (CFA) in mice on the variability of temperature and bioimpedance as an index of pain produced by inflammation. To this end, we initially determined mathematical scores based on changes in temperature and bioimpedance (STB) for animals with an inflamed paw and compared these scores with commonly used measures of inflammatory pain. We then pharmacologically validated the tool using dexamethasone. Results: The STB analysis resembled the response found in the von Frey Hair (vFH) test. The CFA-induced increase in STB and vFH tests were reversed by intraperitoneal administration of dexamethasone. The correlation between the STB and vFH measurements showed a high correlation coefficient (R2=0.911, p < 0.001). Comparison with existing method: Our results also demonstrated that CFA paw injection induced mechanical hyperalgesia in mice and remained virtually unaltered during all time-points tested for 5 days, as measured with vFHs. The administration of CFA into the paw induced a large increase in paw volume that was apparent 1 and 5 days after the injection. The CFA injection resulted in a significant (p<0.05) decrease in the response latency to the heat stimulus, as evaluated on day 4 post-CFA injection. Conclusions: The data presented here suggest that STB may provide a novel non-invasive approach for inflammatory pain detection.2019-08-12T13:05:22Z2020-11-26T17:41:47Z2019-08-12T13:05:22Z2020-11-26T17:41:47Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article192 - 196application/pdf0165-0270https://repositorio.animaeducacao.com.br/handle/ANIMA/2679308 f.LondresAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessReitz, FelipeSalm, Daiana CLudtcke, Daniela DeroSantos, Aureo dosTraebert, JeffersonMartins, Daniel Fernandesengreponame:Repositório Universitário da Ânima (RUNA)instname:Ânima Educaçãoinstacron:Ânima2021-08-11T17:40:37Zoai:repositorio.animaeducacao.com.br:ANIMA/2679Repositório InstitucionalPRIhttps://repositorio.animaeducacao.com.br/oai/requestcontato@animaeducacao.com.bropendoar:2021-08-11T17:40:37Repositório Universitário da Ânima (RUNA) - Ânima Educaçãofalse
dc.title.none.fl_str_mv A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice
title A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice
spellingShingle A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice
Reitz, Felipe
Behavioral test
Inflammatory pain
Pain measurements
Thermography
title_short A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice
title_full A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice
title_fullStr A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice
title_full_unstemmed A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice
title_sort A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice
author Reitz, Felipe
author_facet Reitz, Felipe
Salm, Daiana C
Ludtcke, Daniela Dero
Santos, Aureo dos
Traebert, Jefferson
Martins, Daniel Fernandes
author_role author
author2 Salm, Daiana C
Ludtcke, Daniela Dero
Santos, Aureo dos
Traebert, Jefferson
Martins, Daniel Fernandes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Reitz, Felipe
Salm, Daiana C
Ludtcke, Daniela Dero
Santos, Aureo dos
Traebert, Jefferson
Martins, Daniel Fernandes
dc.subject.por.fl_str_mv Behavioral test
Inflammatory pain
Pain measurements
Thermography
topic Behavioral test
Inflammatory pain
Pain measurements
Thermography
description Background: Preclinical studies measure withdrawal responses to evoking thermal and mechanical stimuli instead of the more clinically important spontaneous pain. New method: Therefore, we studied the effect of peripheral inflammation induced by intraplantar administration of complete Freund’s adjuvant (CFA) in mice on the variability of temperature and bioimpedance as an index of pain produced by inflammation. To this end, we initially determined mathematical scores based on changes in temperature and bioimpedance (STB) for animals with an inflamed paw and compared these scores with commonly used measures of inflammatory pain. We then pharmacologically validated the tool using dexamethasone. Results: The STB analysis resembled the response found in the von Frey Hair (vFH) test. The CFA-induced increase in STB and vFH tests were reversed by intraperitoneal administration of dexamethasone. The correlation between the STB and vFH measurements showed a high correlation coefficient (R2=0.911, p < 0.001). Comparison with existing method: Our results also demonstrated that CFA paw injection induced mechanical hyperalgesia in mice and remained virtually unaltered during all time-points tested for 5 days, as measured with vFHs. The administration of CFA into the paw induced a large increase in paw volume that was apparent 1 and 5 days after the injection. The CFA injection resulted in a significant (p<0.05) decrease in the response latency to the heat stimulus, as evaluated on day 4 post-CFA injection. Conclusions: The data presented here suggest that STB may provide a novel non-invasive approach for inflammatory pain detection.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-08-12T13:05:22Z
2019-08-12T13:05:22Z
2020-11-26T17:41:47Z
2020-11-26T17:41:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 0165-0270
https://repositorio.animaeducacao.com.br/handle/ANIMA/2679
identifier_str_mv 0165-0270
url https://repositorio.animaeducacao.com.br/handle/ANIMA/2679
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 308 f.
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 192 - 196
application/pdf
dc.coverage.none.fl_str_mv Londres
dc.source.none.fl_str_mv reponame:Repositório Universitário da Ânima (RUNA)
instname:Ânima Educação
instacron:Ânima
instname_str Ânima Educação
instacron_str Ânima
institution Ânima
reponame_str Repositório Universitário da Ânima (RUNA)
collection Repositório Universitário da Ânima (RUNA)
repository.name.fl_str_mv Repositório Universitário da Ânima (RUNA) - Ânima Educação
repository.mail.fl_str_mv contato@animaeducacao.com.br
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