Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100099 |
Resumo: | ABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect. |
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Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidneycysteaminecystine dimethyl esterkinases activitiesrenal energy metabolismABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect.Academia Brasileira de Ciências2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100099Anais da Academia Brasileira de Ciências v.90 n.1 2018reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201720160348info:eu-repo/semantics/openAccessRECH,VIRGINIA C.MEZZOMO,NATHANA J.ATHAYDES,GENARO A.FEKSA,LUCIANE R.FIGUEIREDO,VANDRÉ C.KESSLER,ADRIANAFRANCESCHI,ITIANE D. DEWANNMACHER,CLOVIS M.D.eng2018-04-25T00:00:00Zoai:scielo:S0001-37652018000100099Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2018-04-25T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
title |
Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
spellingShingle |
Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney RECH,VIRGINIA C. cysteamine cystine dimethyl ester kinases activities renal energy metabolism |
title_short |
Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
title_full |
Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
title_fullStr |
Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
title_full_unstemmed |
Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
title_sort |
Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
author |
RECH,VIRGINIA C. |
author_facet |
RECH,VIRGINIA C. MEZZOMO,NATHANA J. ATHAYDES,GENARO A. FEKSA,LUCIANE R. FIGUEIREDO,VANDRÉ C. KESSLER,ADRIANA FRANCESCHI,ITIANE D. DE WANNMACHER,CLOVIS M.D. |
author_role |
author |
author2 |
MEZZOMO,NATHANA J. ATHAYDES,GENARO A. FEKSA,LUCIANE R. FIGUEIREDO,VANDRÉ C. KESSLER,ADRIANA FRANCESCHI,ITIANE D. DE WANNMACHER,CLOVIS M.D. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
RECH,VIRGINIA C. MEZZOMO,NATHANA J. ATHAYDES,GENARO A. FEKSA,LUCIANE R. FIGUEIREDO,VANDRÉ C. KESSLER,ADRIANA FRANCESCHI,ITIANE D. DE WANNMACHER,CLOVIS M.D. |
dc.subject.por.fl_str_mv |
cysteamine cystine dimethyl ester kinases activities renal energy metabolism |
topic |
cysteamine cystine dimethyl ester kinases activities renal energy metabolism |
description |
ABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100099 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100099 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201720160348 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.90 n.1 2018 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302865261723648 |