Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney

Detalhes bibliográficos
Autor(a) principal: RECH,VIRGINIA C.
Data de Publicação: 2018
Outros Autores: MEZZOMO,NATHANA J., ATHAYDES,GENARO A., FEKSA,LUCIANE R., FIGUEIREDO,VANDRÉ C., KESSLER,ADRIANA, FRANCESCHI,ITIANE D. DE, WANNMACHER,CLOVIS M.D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100099
Resumo: ABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect.
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spelling Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidneycysteaminecystine dimethyl esterkinases activitiesrenal energy metabolismABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect.Academia Brasileira de Ciências2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100099Anais da Academia Brasileira de Ciências v.90 n.1 2018reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201720160348info:eu-repo/semantics/openAccessRECH,VIRGINIA C.MEZZOMO,NATHANA J.ATHAYDES,GENARO A.FEKSA,LUCIANE R.FIGUEIREDO,VANDRÉ C.KESSLER,ADRIANAFRANCESCHI,ITIANE D. DEWANNMACHER,CLOVIS M.D.eng2018-04-25T00:00:00Zoai:scielo:S0001-37652018000100099Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2018-04-25T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
title Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
spellingShingle Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
RECH,VIRGINIA C.
cysteamine
cystine dimethyl ester
kinases activities
renal energy metabolism
title_short Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
title_full Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
title_fullStr Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
title_full_unstemmed Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
title_sort Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
author RECH,VIRGINIA C.
author_facet RECH,VIRGINIA C.
MEZZOMO,NATHANA J.
ATHAYDES,GENARO A.
FEKSA,LUCIANE R.
FIGUEIREDO,VANDRÉ C.
KESSLER,ADRIANA
FRANCESCHI,ITIANE D. DE
WANNMACHER,CLOVIS M.D.
author_role author
author2 MEZZOMO,NATHANA J.
ATHAYDES,GENARO A.
FEKSA,LUCIANE R.
FIGUEIREDO,VANDRÉ C.
KESSLER,ADRIANA
FRANCESCHI,ITIANE D. DE
WANNMACHER,CLOVIS M.D.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv RECH,VIRGINIA C.
MEZZOMO,NATHANA J.
ATHAYDES,GENARO A.
FEKSA,LUCIANE R.
FIGUEIREDO,VANDRÉ C.
KESSLER,ADRIANA
FRANCESCHI,ITIANE D. DE
WANNMACHER,CLOVIS M.D.
dc.subject.por.fl_str_mv cysteamine
cystine dimethyl ester
kinases activities
renal energy metabolism
topic cysteamine
cystine dimethyl ester
kinases activities
renal energy metabolism
description ABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100099
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100099
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201720160348
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.90 n.1 2018
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
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reponame_str Anais da Academia Brasileira de Ciências (Online)
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repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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