Establishment of an environmental microbiological monitoring program in a mice barrier facility

Detalhes bibliográficos
Autor(a) principal: SCHLAPP,GERALDINE
Data de Publicação: 2018
Outros Autores: FERNÁNDEZ-GRAÑA,GABRIEL, ARÉVALO,ANA PAULA, CRISPO,MARTINA
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603155
Resumo: Abstract Rodents used in biomedical research are maintained behind barriers to exclude microbial contaminants. Several check points have to be monitored to eliminate the potential of introducing adventitious agents into the facility. Microbiological monitoring of a mouse facility environment enables to evaluate the efficiency of sanitization and cleaning procedures, air quality, and technician good practices. At our SPF mouse facility, we implemented an environmental microbiological monitoring program based in sedimentation and swabbing, inexpensive and easy to use methods. The aim of this work was to evaluate the results and the efficiency of the monitoring program after seven years. The median for bacteria and fungi counts in the SPF sampled areas was ≤2 CFU/2 h for settle plates and <1 CFU per swabbing plate, satisfying the requirements for grade C of the EU-GMP, with some modifications. The environmental monitoring program was useful to detect early warning of problems and enabled us to define a safe range of microbiological counts. In addition, SPF status defined for our mice was maintained throughout this study, confirmed by our HM program. This work could encourage directors and technicians of other mouse facilities in Latin America and rest of the world to implement this kind of program.
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spelling Establishment of an environmental microbiological monitoring program in a mice barrier facilityenvironmental microbiological monitoring programhealth monitoringsettle platesSPF mice facilityswabbingAbstract Rodents used in biomedical research are maintained behind barriers to exclude microbial contaminants. Several check points have to be monitored to eliminate the potential of introducing adventitious agents into the facility. Microbiological monitoring of a mouse facility environment enables to evaluate the efficiency of sanitization and cleaning procedures, air quality, and technician good practices. At our SPF mouse facility, we implemented an environmental microbiological monitoring program based in sedimentation and swabbing, inexpensive and easy to use methods. The aim of this work was to evaluate the results and the efficiency of the monitoring program after seven years. The median for bacteria and fungi counts in the SPF sampled areas was ≤2 CFU/2 h for settle plates and <1 CFU per swabbing plate, satisfying the requirements for grade C of the EU-GMP, with some modifications. The environmental monitoring program was useful to detect early warning of problems and enabled us to define a safe range of microbiological counts. In addition, SPF status defined for our mice was maintained throughout this study, confirmed by our HM program. This work could encourage directors and technicians of other mouse facilities in Latin America and rest of the world to implement this kind of program.Academia Brasileira de Ciências2018-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603155Anais da Academia Brasileira de Ciências v.90 n.3 2018reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201820180043info:eu-repo/semantics/openAccessSCHLAPP,GERALDINEFERNÁNDEZ-GRAÑA,GABRIELARÉVALO,ANA PAULACRISPO,MARTINAeng2019-11-29T00:00:00Zoai:scielo:S0001-37652018000603155Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-11-29T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Establishment of an environmental microbiological monitoring program in a mice barrier facility
title Establishment of an environmental microbiological monitoring program in a mice barrier facility
spellingShingle Establishment of an environmental microbiological monitoring program in a mice barrier facility
SCHLAPP,GERALDINE
environmental microbiological monitoring program
health monitoring
settle plates
SPF mice facility
swabbing
title_short Establishment of an environmental microbiological monitoring program in a mice barrier facility
title_full Establishment of an environmental microbiological monitoring program in a mice barrier facility
title_fullStr Establishment of an environmental microbiological monitoring program in a mice barrier facility
title_full_unstemmed Establishment of an environmental microbiological monitoring program in a mice barrier facility
title_sort Establishment of an environmental microbiological monitoring program in a mice barrier facility
author SCHLAPP,GERALDINE
author_facet SCHLAPP,GERALDINE
FERNÁNDEZ-GRAÑA,GABRIEL
ARÉVALO,ANA PAULA
CRISPO,MARTINA
author_role author
author2 FERNÁNDEZ-GRAÑA,GABRIEL
ARÉVALO,ANA PAULA
CRISPO,MARTINA
author2_role author
author
author
dc.contributor.author.fl_str_mv SCHLAPP,GERALDINE
FERNÁNDEZ-GRAÑA,GABRIEL
ARÉVALO,ANA PAULA
CRISPO,MARTINA
dc.subject.por.fl_str_mv environmental microbiological monitoring program
health monitoring
settle plates
SPF mice facility
swabbing
topic environmental microbiological monitoring program
health monitoring
settle plates
SPF mice facility
swabbing
description Abstract Rodents used in biomedical research are maintained behind barriers to exclude microbial contaminants. Several check points have to be monitored to eliminate the potential of introducing adventitious agents into the facility. Microbiological monitoring of a mouse facility environment enables to evaluate the efficiency of sanitization and cleaning procedures, air quality, and technician good practices. At our SPF mouse facility, we implemented an environmental microbiological monitoring program based in sedimentation and swabbing, inexpensive and easy to use methods. The aim of this work was to evaluate the results and the efficiency of the monitoring program after seven years. The median for bacteria and fungi counts in the SPF sampled areas was ≤2 CFU/2 h for settle plates and <1 CFU per swabbing plate, satisfying the requirements for grade C of the EU-GMP, with some modifications. The environmental monitoring program was useful to detect early warning of problems and enabled us to define a safe range of microbiological counts. In addition, SPF status defined for our mice was maintained throughout this study, confirmed by our HM program. This work could encourage directors and technicians of other mouse facilities in Latin America and rest of the world to implement this kind of program.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603155
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603155
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201820180043
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.90 n.3 2018
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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