Cellular bases of hypofractionated radiotherapy protocols for lung cancer

Detalhes bibliográficos
Autor(a) principal: OCOLOTOBICHE,ELIANA EVELINA
Data de Publicação: 2022
Outros Autores: BANEGAS,YULIANA CATALINA, FERRARIS,GUSTAVO, MARTÍNEZ,MARCELO, GÜERCI,ALBA MABEL
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000600601
Resumo: Abstract The extreme demand on health systems due to the COVID-19 pandemic has led to reconsider hypofractionation. Although the best clinical efficacy of these schemes is being demonstrated, the biological bases have not been established. Thus, after validating basic clinical parameters, through complementary in vitro models, we characterized the cellular and molecular mechanisms of hypofractionation protocols. Cell cultures of human lung cancer cell line A549 were irradiated with 0, 2, 4, 8, 12, 16 and 20 Gy. The clastogenic, cytotoxic, proliferative and clonogenic capacities and bystander effect were evaluated. In addition, we assessed survival and toxicity in a retrospective study of 49 patients with lung cancer. Our findings showed that the greater efficacy of ablative regimens should not only be attributed to events of direct cell death induced by genotoxic damage, but also to a lower cell repopulation and the indirect action of clastogenic factors secreted. These treatments were optimal in terms of 1- and 2-year overall survival (74 and 65%, respectively), and progression-free survival at 1 and 2 years (71 and 61%, respectively). The greater efficacy of high doses per fraction could be attributed to a multifactorial mechanism that goes beyond the 4Rs of conventional radiotherapy.
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spelling Cellular bases of hypofractionated radiotherapy protocols for lung cancerradiotherapy dose hypofractionation4RsLinear-Quadratic Modelcytotoxic effectstereotactic body radiotherapyAbstract The extreme demand on health systems due to the COVID-19 pandemic has led to reconsider hypofractionation. Although the best clinical efficacy of these schemes is being demonstrated, the biological bases have not been established. Thus, after validating basic clinical parameters, through complementary in vitro models, we characterized the cellular and molecular mechanisms of hypofractionation protocols. Cell cultures of human lung cancer cell line A549 were irradiated with 0, 2, 4, 8, 12, 16 and 20 Gy. The clastogenic, cytotoxic, proliferative and clonogenic capacities and bystander effect were evaluated. In addition, we assessed survival and toxicity in a retrospective study of 49 patients with lung cancer. Our findings showed that the greater efficacy of ablative regimens should not only be attributed to events of direct cell death induced by genotoxic damage, but also to a lower cell repopulation and the indirect action of clastogenic factors secreted. These treatments were optimal in terms of 1- and 2-year overall survival (74 and 65%, respectively), and progression-free survival at 1 and 2 years (71 and 61%, respectively). The greater efficacy of high doses per fraction could be attributed to a multifactorial mechanism that goes beyond the 4Rs of conventional radiotherapy.Academia Brasileira de Ciências2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000600601Anais da Academia Brasileira de Ciências v.94 n.4 2022reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202220210056info:eu-repo/semantics/openAccessOCOLOTOBICHE,ELIANA EVELINABANEGAS,YULIANA CATALINAFERRARIS,GUSTAVOMARTÍNEZ,MARCELOGÜERCI,ALBA MABELeng2022-07-19T00:00:00Zoai:scielo:S0001-37652022000600601Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2022-07-19T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Cellular bases of hypofractionated radiotherapy protocols for lung cancer
title Cellular bases of hypofractionated radiotherapy protocols for lung cancer
spellingShingle Cellular bases of hypofractionated radiotherapy protocols for lung cancer
OCOLOTOBICHE,ELIANA EVELINA
radiotherapy dose hypofractionation
4Rs
Linear-Quadratic Model
cytotoxic effect
stereotactic body radiotherapy
title_short Cellular bases of hypofractionated radiotherapy protocols for lung cancer
title_full Cellular bases of hypofractionated radiotherapy protocols for lung cancer
title_fullStr Cellular bases of hypofractionated radiotherapy protocols for lung cancer
title_full_unstemmed Cellular bases of hypofractionated radiotherapy protocols for lung cancer
title_sort Cellular bases of hypofractionated radiotherapy protocols for lung cancer
author OCOLOTOBICHE,ELIANA EVELINA
author_facet OCOLOTOBICHE,ELIANA EVELINA
BANEGAS,YULIANA CATALINA
FERRARIS,GUSTAVO
MARTÍNEZ,MARCELO
GÜERCI,ALBA MABEL
author_role author
author2 BANEGAS,YULIANA CATALINA
FERRARIS,GUSTAVO
MARTÍNEZ,MARCELO
GÜERCI,ALBA MABEL
author2_role author
author
author
author
dc.contributor.author.fl_str_mv OCOLOTOBICHE,ELIANA EVELINA
BANEGAS,YULIANA CATALINA
FERRARIS,GUSTAVO
MARTÍNEZ,MARCELO
GÜERCI,ALBA MABEL
dc.subject.por.fl_str_mv radiotherapy dose hypofractionation
4Rs
Linear-Quadratic Model
cytotoxic effect
stereotactic body radiotherapy
topic radiotherapy dose hypofractionation
4Rs
Linear-Quadratic Model
cytotoxic effect
stereotactic body radiotherapy
description Abstract The extreme demand on health systems due to the COVID-19 pandemic has led to reconsider hypofractionation. Although the best clinical efficacy of these schemes is being demonstrated, the biological bases have not been established. Thus, after validating basic clinical parameters, through complementary in vitro models, we characterized the cellular and molecular mechanisms of hypofractionation protocols. Cell cultures of human lung cancer cell line A549 were irradiated with 0, 2, 4, 8, 12, 16 and 20 Gy. The clastogenic, cytotoxic, proliferative and clonogenic capacities and bystander effect were evaluated. In addition, we assessed survival and toxicity in a retrospective study of 49 patients with lung cancer. Our findings showed that the greater efficacy of ablative regimens should not only be attributed to events of direct cell death induced by genotoxic damage, but also to a lower cell repopulation and the indirect action of clastogenic factors secreted. These treatments were optimal in terms of 1- and 2-year overall survival (74 and 65%, respectively), and progression-free survival at 1 and 2 years (71 and 61%, respectively). The greater efficacy of high doses per fraction could be attributed to a multifactorial mechanism that goes beyond the 4Rs of conventional radiotherapy.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000600601
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000600601
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765202220210056
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.94 n.4 2022
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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