Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200881 |
Resumo: | The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits. |
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Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branchesantioxidant capacityapocynaceaechlorogenic acidTabernaemontana catharinensisThe antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.Academia Brasileira de Ciências2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200881Anais da Academia Brasileira de Ciências v.86 n.2 2014reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201420120020info:eu-repo/semantics/openAccessPIANA,MARIANABOLIGON,ALINE A.BRUM,THIELE F. DEZADRA,MARINABELKE,BIANCA V.FROEDER,AMANDA L.F.FROHLICH,JANAÍNA K.NUNES,LETÍCIA T.PAPPIS,LAURENBOLIGON,ALEXANDRA A.ATHAYDE,MARGARETH L.eng2015-10-26T00:00:00Zoai:scielo:S0001-37652014000200881Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-10-26T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches |
title |
Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches |
spellingShingle |
Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches PIANA,MARIANA antioxidant capacity apocynaceae chlorogenic acid Tabernaemontana catharinensis |
title_short |
Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches |
title_full |
Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches |
title_fullStr |
Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches |
title_full_unstemmed |
Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches |
title_sort |
Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches |
author |
PIANA,MARIANA |
author_facet |
PIANA,MARIANA BOLIGON,ALINE A. BRUM,THIELE F. DE ZADRA,MARINA BELKE,BIANCA V. FROEDER,AMANDA L.F. FROHLICH,JANAÍNA K. NUNES,LETÍCIA T. PAPPIS,LAUREN BOLIGON,ALEXANDRA A. ATHAYDE,MARGARETH L. |
author_role |
author |
author2 |
BOLIGON,ALINE A. BRUM,THIELE F. DE ZADRA,MARINA BELKE,BIANCA V. FROEDER,AMANDA L.F. FROHLICH,JANAÍNA K. NUNES,LETÍCIA T. PAPPIS,LAUREN BOLIGON,ALEXANDRA A. ATHAYDE,MARGARETH L. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
PIANA,MARIANA BOLIGON,ALINE A. BRUM,THIELE F. DE ZADRA,MARINA BELKE,BIANCA V. FROEDER,AMANDA L.F. FROHLICH,JANAÍNA K. NUNES,LETÍCIA T. PAPPIS,LAUREN BOLIGON,ALEXANDRA A. ATHAYDE,MARGARETH L. |
dc.subject.por.fl_str_mv |
antioxidant capacity apocynaceae chlorogenic acid Tabernaemontana catharinensis |
topic |
antioxidant capacity apocynaceae chlorogenic acid Tabernaemontana catharinensis |
description |
The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200881 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200881 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201420120020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.86 n.2 2014 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302860008357888 |