Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)

Detalhes bibliográficos
Autor(a) principal: PEREIRA,FERNANDA B.
Data de Publicação: 2021
Outros Autores: SEBBENN,ALEXANDRE M., ROSSINI,BRUNO C., MELCHERT,GUILHERME F., MARINO,CELSO L., RIBOLLA,PAULO E.M., ALONSO,DIEGO P., VIDAL,EDSON, TAMBARUSSI,EVANDRO V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300809
Resumo: Abstract Microsatellite primers pairs were developed for the Neotropical tree Roupala montana var. brasiliensis for use in studies on genetic diversity, mating system, and gene flow. Forty-two primer pairs were developed, resulting in 27 polymorphic loci, with two to 27 alleles per locus. The primer pairs were validated against 34 R. montana var. brasiliensis adult trees from four populations. The observed (H o) and expected (H e)heterozygosities ranged among loci from 0.061 to 0.930 (mean of 0.544) and from 0.116 to 0.950 (mean of 0.700), respectively. Null alleles were observed for ten loci. No genotypic linkage disequilibrium was detected in any pair of loci. This set of loci is suitable for population genetic studies of the species.
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spelling Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)Brazilian lacewoodBrazilian oakHigh-throughput sequencingSSR markersAbstract Microsatellite primers pairs were developed for the Neotropical tree Roupala montana var. brasiliensis for use in studies on genetic diversity, mating system, and gene flow. Forty-two primer pairs were developed, resulting in 27 polymorphic loci, with two to 27 alleles per locus. The primer pairs were validated against 34 R. montana var. brasiliensis adult trees from four populations. The observed (H o) and expected (H e)heterozygosities ranged among loci from 0.061 to 0.930 (mean of 0.544) and from 0.116 to 0.950 (mean of 0.700), respectively. Null alleles were observed for ten loci. No genotypic linkage disequilibrium was detected in any pair of loci. This set of loci is suitable for population genetic studies of the species.Academia Brasileira de Ciências2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300809Anais da Academia Brasileira de Ciências v.93 n.2 2021reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202120200452info:eu-repo/semantics/openAccessPEREIRA,FERNANDA B.SEBBENN,ALEXANDRE M.ROSSINI,BRUNO C.MELCHERT,GUILHERME F.MARINO,CELSO L.RIBOLLA,PAULO E.M.ALONSO,DIEGO P.VIDAL,EDSONTAMBARUSSI,EVANDRO V.eng2021-05-26T00:00:00Zoai:scielo:S0001-37652021000300809Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2021-05-26T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)
title Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)
spellingShingle Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)
PEREIRA,FERNANDA B.
Brazilian lacewood
Brazilian oak
High-throughput sequencing
SSR markers
title_short Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)
title_full Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)
title_fullStr Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)
title_full_unstemmed Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)
title_sort Isolation of 27 polymorphic nuclear microsatellite markers for Roupala montana var. brasiliensis (Proteaceae)
author PEREIRA,FERNANDA B.
author_facet PEREIRA,FERNANDA B.
SEBBENN,ALEXANDRE M.
ROSSINI,BRUNO C.
MELCHERT,GUILHERME F.
MARINO,CELSO L.
RIBOLLA,PAULO E.M.
ALONSO,DIEGO P.
VIDAL,EDSON
TAMBARUSSI,EVANDRO V.
author_role author
author2 SEBBENN,ALEXANDRE M.
ROSSINI,BRUNO C.
MELCHERT,GUILHERME F.
MARINO,CELSO L.
RIBOLLA,PAULO E.M.
ALONSO,DIEGO P.
VIDAL,EDSON
TAMBARUSSI,EVANDRO V.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv PEREIRA,FERNANDA B.
SEBBENN,ALEXANDRE M.
ROSSINI,BRUNO C.
MELCHERT,GUILHERME F.
MARINO,CELSO L.
RIBOLLA,PAULO E.M.
ALONSO,DIEGO P.
VIDAL,EDSON
TAMBARUSSI,EVANDRO V.
dc.subject.por.fl_str_mv Brazilian lacewood
Brazilian oak
High-throughput sequencing
SSR markers
topic Brazilian lacewood
Brazilian oak
High-throughput sequencing
SSR markers
description Abstract Microsatellite primers pairs were developed for the Neotropical tree Roupala montana var. brasiliensis for use in studies on genetic diversity, mating system, and gene flow. Forty-two primer pairs were developed, resulting in 27 polymorphic loci, with two to 27 alleles per locus. The primer pairs were validated against 34 R. montana var. brasiliensis adult trees from four populations. The observed (H o) and expected (H e)heterozygosities ranged among loci from 0.061 to 0.930 (mean of 0.544) and from 0.116 to 0.950 (mean of 0.700), respectively. Null alleles were observed for ten loci. No genotypic linkage disequilibrium was detected in any pair of loci. This set of loci is suitable for population genetic studies of the species.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300809
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300809
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765202120200452
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.93 n.2 2021
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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