Caspase-3 activation and increased procollagen type I in irradiated hearts

Detalhes bibliográficos
Autor(a) principal: Ferreira-Machado,Samara C.
Data de Publicação: 2013
Outros Autores: Salata,Camila, Rocha,Nazareth N., Corrêa,Alexandre F.S., Côrte-Real,Suzana, Peregrino,Antônio A.F., Campos,Vera M.A. de, Andrade,Cherley B.V., Bernardo-Filho,Mario, Cabral-Neto,Januário B., Dealmeida,Carlos E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100215
Resumo: The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and functional (reduced ejection fraction) were observed. Data suggest that the cardiac function decline after exposure to ionizing radiation is related, in part, to increased collagen and increased caspase-3-cleaved.
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spelling Caspase-3 activation and increased procollagen type I in irradiated heartsapoptosiscaspase-3fibrosisheartionizing radiationTGF-β1The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and functional (reduced ejection fraction) were observed. Data suggest that the cardiac function decline after exposure to ionizing radiation is related, in part, to increased collagen and increased caspase-3-cleaved.Academia Brasileira de Ciências2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100215Anais da Academia Brasileira de Ciências v.85 n.1 2013reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/S0001-37652013005000009info:eu-repo/semantics/openAccessFerreira-Machado,Samara C.Salata,CamilaRocha,Nazareth N.Corrêa,Alexandre F.S.Côrte-Real,SuzanaPeregrino,Antônio A.F.Campos,Vera M.A. deAndrade,Cherley B.V.Bernardo-Filho,MarioCabral-Neto,Januário B.Dealmeida,Carlos E.eng2015-10-19T00:00:00Zoai:scielo:S0001-37652013000100215Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-10-19T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Caspase-3 activation and increased procollagen type I in irradiated hearts
title Caspase-3 activation and increased procollagen type I in irradiated hearts
spellingShingle Caspase-3 activation and increased procollagen type I in irradiated hearts
Ferreira-Machado,Samara C.
apoptosis
caspase-3
fibrosis
heart
ionizing radiation
TGF-β1
title_short Caspase-3 activation and increased procollagen type I in irradiated hearts
title_full Caspase-3 activation and increased procollagen type I in irradiated hearts
title_fullStr Caspase-3 activation and increased procollagen type I in irradiated hearts
title_full_unstemmed Caspase-3 activation and increased procollagen type I in irradiated hearts
title_sort Caspase-3 activation and increased procollagen type I in irradiated hearts
author Ferreira-Machado,Samara C.
author_facet Ferreira-Machado,Samara C.
Salata,Camila
Rocha,Nazareth N.
Corrêa,Alexandre F.S.
Côrte-Real,Suzana
Peregrino,Antônio A.F.
Campos,Vera M.A. de
Andrade,Cherley B.V.
Bernardo-Filho,Mario
Cabral-Neto,Januário B.
Dealmeida,Carlos E.
author_role author
author2 Salata,Camila
Rocha,Nazareth N.
Corrêa,Alexandre F.S.
Côrte-Real,Suzana
Peregrino,Antônio A.F.
Campos,Vera M.A. de
Andrade,Cherley B.V.
Bernardo-Filho,Mario
Cabral-Neto,Januário B.
Dealmeida,Carlos E.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira-Machado,Samara C.
Salata,Camila
Rocha,Nazareth N.
Corrêa,Alexandre F.S.
Côrte-Real,Suzana
Peregrino,Antônio A.F.
Campos,Vera M.A. de
Andrade,Cherley B.V.
Bernardo-Filho,Mario
Cabral-Neto,Januário B.
Dealmeida,Carlos E.
dc.subject.por.fl_str_mv apoptosis
caspase-3
fibrosis
heart
ionizing radiation
TGF-β1
topic apoptosis
caspase-3
fibrosis
heart
ionizing radiation
TGF-β1
description The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and functional (reduced ejection fraction) were observed. Data suggest that the cardiac function decline after exposure to ionizing radiation is related, in part, to increased collagen and increased caspase-3-cleaved.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100215
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100215
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0001-37652013005000009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.85 n.1 2013
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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