Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species

Detalhes bibliográficos
Autor(a) principal: SCORZONI,LILIANA
Data de Publicação: 2020
Outros Autores: MENEZES,RAQUEL T. DE, PEREIRA,THAIS C., OLIVEIRA,PRISCILA S., RIBEIRO,FELIPE DE CAMARGO, SANTOS,EVELYN LUZIA DE SOUZA, FUGISAKI,LUCIANA R.O., OLIVEIRA,LUCIANE D. DE, AMORIM,JOSÉ BENEDITO O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700935
Resumo: Abstract Candida is a human fungal pathogen that causes a wide range of diseases. Candida albicans is the main etiologic agent in these diseases; however, infections can be caused by non-albicans Candida species. Virulence factors such as biofilm production, which protect the fungus from host immunity and anti-fungal drugs, are important for the infection. Therefore, available antifungal drugs for candidiasis treatment are limited and the investigation of new and effective drugs is needed. Verapamil is a calcium channel blocker with an inhibitory effect on hyphae development, adhesion, and colonization of C. albicans. In this study, we investigated the effect of verapamil on cell viability and its antifungal and anti-biofilm activity in non-albicans Candida species. Verapamil was not toxic to keratinocyte cells; moreover, C. krusei, C. parapsilosis, and C. glabrata were susceptible to verapamil with a minimal inhibitory concentration (MIC) of 1250 μM; in addition, this drug displayed fungistatic effect at the evaluated concentrations. After treatment with verapamil, reduced viability, biomass, and mitochondrial activity were observed in biofilms of the non-albicans Candida species C. krusei, C. glabrata, and C. parapsilosis. These findings highlight the importance of the study of verapamil as an alternative treatment for infections caused by non-albicans Candida species.
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spelling Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida speciesVerapamilantifungal activitybiofilmCandida non-albicansdrug repurposingAbstract Candida is a human fungal pathogen that causes a wide range of diseases. Candida albicans is the main etiologic agent in these diseases; however, infections can be caused by non-albicans Candida species. Virulence factors such as biofilm production, which protect the fungus from host immunity and anti-fungal drugs, are important for the infection. Therefore, available antifungal drugs for candidiasis treatment are limited and the investigation of new and effective drugs is needed. Verapamil is a calcium channel blocker with an inhibitory effect on hyphae development, adhesion, and colonization of C. albicans. In this study, we investigated the effect of verapamil on cell viability and its antifungal and anti-biofilm activity in non-albicans Candida species. Verapamil was not toxic to keratinocyte cells; moreover, C. krusei, C. parapsilosis, and C. glabrata were susceptible to verapamil with a minimal inhibitory concentration (MIC) of 1250 μM; in addition, this drug displayed fungistatic effect at the evaluated concentrations. After treatment with verapamil, reduced viability, biomass, and mitochondrial activity were observed in biofilms of the non-albicans Candida species C. krusei, C. glabrata, and C. parapsilosis. These findings highlight the importance of the study of verapamil as an alternative treatment for infections caused by non-albicans Candida species.Academia Brasileira de Ciências2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700935Anais da Academia Brasileira de Ciências v.92 n.4 2020reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202020200703info:eu-repo/semantics/openAccessSCORZONI,LILIANAMENEZES,RAQUEL T. DEPEREIRA,THAIS C.OLIVEIRA,PRISCILA S.RIBEIRO,FELIPE DE CAMARGOSANTOS,EVELYN LUZIA DE SOUZAFUGISAKI,LUCIANA R.O.OLIVEIRA,LUCIANE D. DEAMORIM,JOSÉ BENEDITO O.eng2020-12-11T00:00:00Zoai:scielo:S0001-37652020000700935Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2020-12-11T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species
title Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species
spellingShingle Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species
SCORZONI,LILIANA
Verapamil
antifungal activity
biofilm
Candida non-albicans
drug repurposing
title_short Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species
title_full Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species
title_fullStr Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species
title_full_unstemmed Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species
title_sort Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species
author SCORZONI,LILIANA
author_facet SCORZONI,LILIANA
MENEZES,RAQUEL T. DE
PEREIRA,THAIS C.
OLIVEIRA,PRISCILA S.
RIBEIRO,FELIPE DE CAMARGO
SANTOS,EVELYN LUZIA DE SOUZA
FUGISAKI,LUCIANA R.O.
OLIVEIRA,LUCIANE D. DE
AMORIM,JOSÉ BENEDITO O.
author_role author
author2 MENEZES,RAQUEL T. DE
PEREIRA,THAIS C.
OLIVEIRA,PRISCILA S.
RIBEIRO,FELIPE DE CAMARGO
SANTOS,EVELYN LUZIA DE SOUZA
FUGISAKI,LUCIANA R.O.
OLIVEIRA,LUCIANE D. DE
AMORIM,JOSÉ BENEDITO O.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv SCORZONI,LILIANA
MENEZES,RAQUEL T. DE
PEREIRA,THAIS C.
OLIVEIRA,PRISCILA S.
RIBEIRO,FELIPE DE CAMARGO
SANTOS,EVELYN LUZIA DE SOUZA
FUGISAKI,LUCIANA R.O.
OLIVEIRA,LUCIANE D. DE
AMORIM,JOSÉ BENEDITO O.
dc.subject.por.fl_str_mv Verapamil
antifungal activity
biofilm
Candida non-albicans
drug repurposing
topic Verapamil
antifungal activity
biofilm
Candida non-albicans
drug repurposing
description Abstract Candida is a human fungal pathogen that causes a wide range of diseases. Candida albicans is the main etiologic agent in these diseases; however, infections can be caused by non-albicans Candida species. Virulence factors such as biofilm production, which protect the fungus from host immunity and anti-fungal drugs, are important for the infection. Therefore, available antifungal drugs for candidiasis treatment are limited and the investigation of new and effective drugs is needed. Verapamil is a calcium channel blocker with an inhibitory effect on hyphae development, adhesion, and colonization of C. albicans. In this study, we investigated the effect of verapamil on cell viability and its antifungal and anti-biofilm activity in non-albicans Candida species. Verapamil was not toxic to keratinocyte cells; moreover, C. krusei, C. parapsilosis, and C. glabrata were susceptible to verapamil with a minimal inhibitory concentration (MIC) of 1250 μM; in addition, this drug displayed fungistatic effect at the evaluated concentrations. After treatment with verapamil, reduced viability, biomass, and mitochondrial activity were observed in biofilms of the non-albicans Candida species C. krusei, C. glabrata, and C. parapsilosis. These findings highlight the importance of the study of verapamil as an alternative treatment for infections caused by non-albicans Candida species.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700935
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700935
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765202020200703
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.92 n.4 2020
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
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instname_str Academia Brasileira de Ciências (ABC)
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reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
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