Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes

Detalhes bibliográficos
Autor(a) principal: Esteves,Mabel B.
Data de Publicação: 2005
Outros Autores: Marques-Santos,Luis F., Affonso-Mitidieri,Ottília R., Rumjanek,Vivian M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000200008
Resumo: Lymphocytes activated by mitogenic lectins display changes in transmembrane potential, an elevation in the cytoplasmic Ca2+ concentrations, proliferation and/or activation induced cell death. Low concentrations of ouabain (an inhibitor of Na+,K+-ATPase) suppress mitogen-induced proliferation and increases cell death. To understand the mechanisms involved, a number of parameters were analyzed using fluorescent probes and flow cytometry. The addition of 100nM ouabain to cultures of peripheral blood lymphocytes activated with 5µg/ml phytohemagglutinin (PHA) did not modify the increased expression of the Fas receptor or its ligand FasL induced by the mitogen. However, treatment with ouabain potentiated apoptosis induced by an anti-Fas agonist antibody. A synergy between ouabain and PHA was also observed with regard to plasma membrane depolarization. PHA per se did not induce dissipation of mitochondrial membrane potential but when cells were also exposed to ouabain a marked depolarization could be observed, and this was a late event. It is possible that the inhibitory effect of ouabain on activated peripheral blood lymphocytes involves the potentiation of some of the steps of the apoptotic process and reflects an exacerbation of the mechanism of activation-induced cell death.
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spelling Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocyteslymphocytesouabainPHAapoptosismembrane depolarizationLymphocytes activated by mitogenic lectins display changes in transmembrane potential, an elevation in the cytoplasmic Ca2+ concentrations, proliferation and/or activation induced cell death. Low concentrations of ouabain (an inhibitor of Na+,K+-ATPase) suppress mitogen-induced proliferation and increases cell death. To understand the mechanisms involved, a number of parameters were analyzed using fluorescent probes and flow cytometry. The addition of 100nM ouabain to cultures of peripheral blood lymphocytes activated with 5µg/ml phytohemagglutinin (PHA) did not modify the increased expression of the Fas receptor or its ligand FasL induced by the mitogen. However, treatment with ouabain potentiated apoptosis induced by an anti-Fas agonist antibody. A synergy between ouabain and PHA was also observed with regard to plasma membrane depolarization. PHA per se did not induce dissipation of mitochondrial membrane potential but when cells were also exposed to ouabain a marked depolarization could be observed, and this was a late event. It is possible that the inhibitory effect of ouabain on activated peripheral blood lymphocytes involves the potentiation of some of the steps of the apoptotic process and reflects an exacerbation of the mechanism of activation-induced cell death.Academia Brasileira de Ciências2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000200008Anais da Academia Brasileira de Ciências v.77 n.2 2005reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/S0001-37652005000200008info:eu-repo/semantics/openAccessEsteves,Mabel B.Marques-Santos,Luis F.Affonso-Mitidieri,Ottília R.Rumjanek,Vivian M.eng2005-05-09T00:00:00Zoai:scielo:S0001-37652005000200008Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2005-05-09T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
title Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
spellingShingle Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
Esteves,Mabel B.
lymphocytes
ouabain
PHA
apoptosis
membrane depolarization
title_short Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
title_full Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
title_fullStr Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
title_full_unstemmed Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
title_sort Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
author Esteves,Mabel B.
author_facet Esteves,Mabel B.
Marques-Santos,Luis F.
Affonso-Mitidieri,Ottília R.
Rumjanek,Vivian M.
author_role author
author2 Marques-Santos,Luis F.
Affonso-Mitidieri,Ottília R.
Rumjanek,Vivian M.
author2_role author
author
author
dc.contributor.author.fl_str_mv Esteves,Mabel B.
Marques-Santos,Luis F.
Affonso-Mitidieri,Ottília R.
Rumjanek,Vivian M.
dc.subject.por.fl_str_mv lymphocytes
ouabain
PHA
apoptosis
membrane depolarization
topic lymphocytes
ouabain
PHA
apoptosis
membrane depolarization
description Lymphocytes activated by mitogenic lectins display changes in transmembrane potential, an elevation in the cytoplasmic Ca2+ concentrations, proliferation and/or activation induced cell death. Low concentrations of ouabain (an inhibitor of Na+,K+-ATPase) suppress mitogen-induced proliferation and increases cell death. To understand the mechanisms involved, a number of parameters were analyzed using fluorescent probes and flow cytometry. The addition of 100nM ouabain to cultures of peripheral blood lymphocytes activated with 5µg/ml phytohemagglutinin (PHA) did not modify the increased expression of the Fas receptor or its ligand FasL induced by the mitogen. However, treatment with ouabain potentiated apoptosis induced by an anti-Fas agonist antibody. A synergy between ouabain and PHA was also observed with regard to plasma membrane depolarization. PHA per se did not induce dissipation of mitochondrial membrane potential but when cells were also exposed to ouabain a marked depolarization could be observed, and this was a late event. It is possible that the inhibitory effect of ouabain on activated peripheral blood lymphocytes involves the potentiation of some of the steps of the apoptotic process and reflects an exacerbation of the mechanism of activation-induced cell death.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000200008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000200008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0001-37652005000200008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.77 n.2 2005
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
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institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
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