Counteracting effects on free radicals and histological alterations induced by a fraction with casearins
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401791 |
Resumo: | ABSTRACTCasearia sylvestris Swartz is a medicinal plant widely distributed in Brazil. It has anti-inflammatory, antiulcer and antitumor activities and is popularly used to treat snakebites, wounds, diarrhea, flu and chest colds. Its leaves are rich in oxygenated tricyclic cis-clerodane diterpenes, particulary casearins. Herein, we evaluated the antioxidant activities of a fraction with casearins (FC) isolated from C. sylvestrisand histological changes on the central nervous system and livers of Mus musculus mice. Firstly, in vitro studies (0.9, 1.8, 3.6, 5.4 and 7.2 μg/mL) revealed EC50 values of 3.7, 6.4 and 0.16 µg/mL for nitrite, hydroxyl radical and TBARS levels, respectively. Secondly, FC (2.5, 5, 10 and 25 mg/kg/day) was intraperitoneally administered to Swiss mice for 7 consecutive days. Nitrite levels in the hippocampus (26.2, 27.3, 30.2 and 26.6 µM) and striatum (26.3, 25.4, 34.3 and 27.5 µM) increased in all treated animals (P < 0.05). Lower doses dropped reduced glutathione, catalase and TBARS levels in the hippocampus and striatum. With the exception of this reduction in TBARS formation, FC displayed only in vitro antioxidant activity. Animals exhibited histological alterations suggestive of neurotoxicity and hepatotoxicity, indicating the need for precaution regarding the consumption of medicinal formulations based on Casearia sylvestris. |
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Counteracting effects on free radicals and histological alterations induced by a fraction with casearinsClerodane diterpenesCasearia sylvestrisantioxidantmorphological changestoxicityABSTRACTCasearia sylvestris Swartz is a medicinal plant widely distributed in Brazil. It has anti-inflammatory, antiulcer and antitumor activities and is popularly used to treat snakebites, wounds, diarrhea, flu and chest colds. Its leaves are rich in oxygenated tricyclic cis-clerodane diterpenes, particulary casearins. Herein, we evaluated the antioxidant activities of a fraction with casearins (FC) isolated from C. sylvestrisand histological changes on the central nervous system and livers of Mus musculus mice. Firstly, in vitro studies (0.9, 1.8, 3.6, 5.4 and 7.2 μg/mL) revealed EC50 values of 3.7, 6.4 and 0.16 µg/mL for nitrite, hydroxyl radical and TBARS levels, respectively. Secondly, FC (2.5, 5, 10 and 25 mg/kg/day) was intraperitoneally administered to Swiss mice for 7 consecutive days. Nitrite levels in the hippocampus (26.2, 27.3, 30.2 and 26.6 µM) and striatum (26.3, 25.4, 34.3 and 27.5 µM) increased in all treated animals (P < 0.05). Lower doses dropped reduced glutathione, catalase and TBARS levels in the hippocampus and striatum. With the exception of this reduction in TBARS formation, FC displayed only in vitro antioxidant activity. Animals exhibited histological alterations suggestive of neurotoxicity and hepatotoxicity, indicating the need for precaution regarding the consumption of medicinal formulations based on Casearia sylvestris.Academia Brasileira de Ciências2015-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401791Anais da Academia Brasileira de Ciências v.87 n.3 2015reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201520150149info:eu-repo/semantics/openAccessARAÚJO,ÉVERTON JOSÉ FERREIRA DEOLIVEIRA,GUILHERME ANTÔNIO LOPES DESOUSA,LÍVIA QUEIROZ DEBOLZANI,VANDERLAN DA SILVACAVALHEIRO,ALBERTO JOSÉTOME,ADRIANA DA ROCHAPERON,ANA PAULASANTOS,ANDRÉ GONZAGA DOSCITÓ,ANTONIA MARIA DAS GRAÇAS LOPESPESSOA,CLÁUDIAFREITAS,RIVELILSON MENDES DEFERREIRA,PAULO MICHEL PINHEIROeng2015-09-22T00:00:00Zoai:scielo:S0001-37652015000401791Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-09-22T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Counteracting effects on free radicals and histological alterations induced by a fraction with casearins |
title |
Counteracting effects on free radicals and histological alterations induced by a fraction with casearins |
spellingShingle |
Counteracting effects on free radicals and histological alterations induced by a fraction with casearins ARAÚJO,ÉVERTON JOSÉ FERREIRA DE Clerodane diterpenes Casearia sylvestris antioxidant morphological changes toxicity |
title_short |
Counteracting effects on free radicals and histological alterations induced by a fraction with casearins |
title_full |
Counteracting effects on free radicals and histological alterations induced by a fraction with casearins |
title_fullStr |
Counteracting effects on free radicals and histological alterations induced by a fraction with casearins |
title_full_unstemmed |
Counteracting effects on free radicals and histological alterations induced by a fraction with casearins |
title_sort |
Counteracting effects on free radicals and histological alterations induced by a fraction with casearins |
author |
ARAÚJO,ÉVERTON JOSÉ FERREIRA DE |
author_facet |
ARAÚJO,ÉVERTON JOSÉ FERREIRA DE OLIVEIRA,GUILHERME ANTÔNIO LOPES DE SOUSA,LÍVIA QUEIROZ DE BOLZANI,VANDERLAN DA SILVA CAVALHEIRO,ALBERTO JOSÉ TOME,ADRIANA DA ROCHA PERON,ANA PAULA SANTOS,ANDRÉ GONZAGA DOS CITÓ,ANTONIA MARIA DAS GRAÇAS LOPES PESSOA,CLÁUDIA FREITAS,RIVELILSON MENDES DE FERREIRA,PAULO MICHEL PINHEIRO |
author_role |
author |
author2 |
OLIVEIRA,GUILHERME ANTÔNIO LOPES DE SOUSA,LÍVIA QUEIROZ DE BOLZANI,VANDERLAN DA SILVA CAVALHEIRO,ALBERTO JOSÉ TOME,ADRIANA DA ROCHA PERON,ANA PAULA SANTOS,ANDRÉ GONZAGA DOS CITÓ,ANTONIA MARIA DAS GRAÇAS LOPES PESSOA,CLÁUDIA FREITAS,RIVELILSON MENDES DE FERREIRA,PAULO MICHEL PINHEIRO |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
ARAÚJO,ÉVERTON JOSÉ FERREIRA DE OLIVEIRA,GUILHERME ANTÔNIO LOPES DE SOUSA,LÍVIA QUEIROZ DE BOLZANI,VANDERLAN DA SILVA CAVALHEIRO,ALBERTO JOSÉ TOME,ADRIANA DA ROCHA PERON,ANA PAULA SANTOS,ANDRÉ GONZAGA DOS CITÓ,ANTONIA MARIA DAS GRAÇAS LOPES PESSOA,CLÁUDIA FREITAS,RIVELILSON MENDES DE FERREIRA,PAULO MICHEL PINHEIRO |
dc.subject.por.fl_str_mv |
Clerodane diterpenes Casearia sylvestris antioxidant morphological changes toxicity |
topic |
Clerodane diterpenes Casearia sylvestris antioxidant morphological changes toxicity |
description |
ABSTRACTCasearia sylvestris Swartz is a medicinal plant widely distributed in Brazil. It has anti-inflammatory, antiulcer and antitumor activities and is popularly used to treat snakebites, wounds, diarrhea, flu and chest colds. Its leaves are rich in oxygenated tricyclic cis-clerodane diterpenes, particulary casearins. Herein, we evaluated the antioxidant activities of a fraction with casearins (FC) isolated from C. sylvestrisand histological changes on the central nervous system and livers of Mus musculus mice. Firstly, in vitro studies (0.9, 1.8, 3.6, 5.4 and 7.2 μg/mL) revealed EC50 values of 3.7, 6.4 and 0.16 µg/mL for nitrite, hydroxyl radical and TBARS levels, respectively. Secondly, FC (2.5, 5, 10 and 25 mg/kg/day) was intraperitoneally administered to Swiss mice for 7 consecutive days. Nitrite levels in the hippocampus (26.2, 27.3, 30.2 and 26.6 µM) and striatum (26.3, 25.4, 34.3 and 27.5 µM) increased in all treated animals (P < 0.05). Lower doses dropped reduced glutathione, catalase and TBARS levels in the hippocampus and striatum. With the exception of this reduction in TBARS formation, FC displayed only in vitro antioxidant activity. Animals exhibited histological alterations suggestive of neurotoxicity and hepatotoxicity, indicating the need for precaution regarding the consumption of medicinal formulations based on Casearia sylvestris. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401791 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401791 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201520150149 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.87 n.3 2015 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302861892648960 |