Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro

Detalhes bibliográficos
Autor(a) principal: LEMOS,VANESSA P.A.
Data de Publicação: 2022
Outros Autores: PORTO,MICHELE, CEZAR,RAFAEL DA S., SANTOS,BRUNO P. DOS, SOUZA,MELISSA R. DE, SILVA,JULIANA DA, NARDI,NANCE B., CAMASSOLA,MELISSA
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700803
Resumo: Abstract Mesenchymal stem cells present clinical potential to recover and regenerate injured tissues in diverse pathologies. The in vitro expansion and characterization of these cells contribute to elucidation of the mechanisms of senescence and strategies involving cell therapies. This study aimed to compare specific characteristics between initial and advanced passages of mesenchymal stem cells derived from adipose tissue and bone marrow. Both cell types were characterized according to immunophenotype, osteogenic differentiation, genomic instability, migration assay, doubling population time and colony forming ability. Our results demonstrated that both cell types were able to maintain an immunophenotypic profile typical of mesenchymal stem cells during increasing passages. Adipose stem cells at initial passage presented greater migration capacity compared to advanced passage cells, and advanced passage cells proliferated faster than initial passage cells. Bone marrow stem cells at early passages presented higher osteogenic potential than advanced. At advanced passages they presented higher colony forming capacity and genetic damage than those at initial passage. These results suggest that mesenchymal stem cells maintained in culture presented characteristics of senescence that should be monitored prior the use in regenerative medicine and cells derived from bone marrow at initial passage have better potential for therapeutic use in bone tissue engineering.
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spelling Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitroosteogenesissenescencestem cellstissue engineeringAbstract Mesenchymal stem cells present clinical potential to recover and regenerate injured tissues in diverse pathologies. The in vitro expansion and characterization of these cells contribute to elucidation of the mechanisms of senescence and strategies involving cell therapies. This study aimed to compare specific characteristics between initial and advanced passages of mesenchymal stem cells derived from adipose tissue and bone marrow. Both cell types were characterized according to immunophenotype, osteogenic differentiation, genomic instability, migration assay, doubling population time and colony forming ability. Our results demonstrated that both cell types were able to maintain an immunophenotypic profile typical of mesenchymal stem cells during increasing passages. Adipose stem cells at initial passage presented greater migration capacity compared to advanced passage cells, and advanced passage cells proliferated faster than initial passage cells. Bone marrow stem cells at early passages presented higher osteogenic potential than advanced. At advanced passages they presented higher colony forming capacity and genetic damage than those at initial passage. These results suggest that mesenchymal stem cells maintained in culture presented characteristics of senescence that should be monitored prior the use in regenerative medicine and cells derived from bone marrow at initial passage have better potential for therapeutic use in bone tissue engineering.Academia Brasileira de Ciências2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700803Anais da Academia Brasileira de Ciências v.94 suppl.3 2022reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202220211246info:eu-repo/semantics/openAccessLEMOS,VANESSA P.A.PORTO,MICHELECEZAR,RAFAEL DA S.SANTOS,BRUNO P. DOSSOUZA,MELISSA R. DESILVA,JULIANA DANARDI,NANCE B.CAMASSOLA,MELISSAeng2022-11-17T00:00:00Zoai:scielo:S0001-37652022000700803Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2022-11-17T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro
title Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro
spellingShingle Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro
LEMOS,VANESSA P.A.
osteogenesis
senescence
stem cells
tissue engineering
title_short Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro
title_full Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro
title_fullStr Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro
title_full_unstemmed Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro
title_sort Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro
author LEMOS,VANESSA P.A.
author_facet LEMOS,VANESSA P.A.
PORTO,MICHELE
CEZAR,RAFAEL DA S.
SANTOS,BRUNO P. DOS
SOUZA,MELISSA R. DE
SILVA,JULIANA DA
NARDI,NANCE B.
CAMASSOLA,MELISSA
author_role author
author2 PORTO,MICHELE
CEZAR,RAFAEL DA S.
SANTOS,BRUNO P. DOS
SOUZA,MELISSA R. DE
SILVA,JULIANA DA
NARDI,NANCE B.
CAMASSOLA,MELISSA
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv LEMOS,VANESSA P.A.
PORTO,MICHELE
CEZAR,RAFAEL DA S.
SANTOS,BRUNO P. DOS
SOUZA,MELISSA R. DE
SILVA,JULIANA DA
NARDI,NANCE B.
CAMASSOLA,MELISSA
dc.subject.por.fl_str_mv osteogenesis
senescence
stem cells
tissue engineering
topic osteogenesis
senescence
stem cells
tissue engineering
description Abstract Mesenchymal stem cells present clinical potential to recover and regenerate injured tissues in diverse pathologies. The in vitro expansion and characterization of these cells contribute to elucidation of the mechanisms of senescence and strategies involving cell therapies. This study aimed to compare specific characteristics between initial and advanced passages of mesenchymal stem cells derived from adipose tissue and bone marrow. Both cell types were characterized according to immunophenotype, osteogenic differentiation, genomic instability, migration assay, doubling population time and colony forming ability. Our results demonstrated that both cell types were able to maintain an immunophenotypic profile typical of mesenchymal stem cells during increasing passages. Adipose stem cells at initial passage presented greater migration capacity compared to advanced passage cells, and advanced passage cells proliferated faster than initial passage cells. Bone marrow stem cells at early passages presented higher osteogenic potential than advanced. At advanced passages they presented higher colony forming capacity and genetic damage than those at initial passage. These results suggest that mesenchymal stem cells maintained in culture presented characteristics of senescence that should be monitored prior the use in regenerative medicine and cells derived from bone marrow at initial passage have better potential for therapeutic use in bone tissue engineering.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700803
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700803
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765202220211246
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.94 suppl.3 2022
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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