In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)

Detalhes bibliográficos
Autor(a) principal: ESTEVAM,ELISÂNGELA B.B.
Data de Publicação: 2017
Outros Autores: DEUS,ISABELLA P.B. DE, SILVA,VANESSA P. DA, SILVA,ELIZABETH A.J. DA, ALVES,CASSIA C.F., ALVES,JOSÉ MILTON, CAZAL,CRISTIANE M., MAGALHÃES,LIZANDRA G., PAGOTTI,MARIANA C., ESPERANDIM,VIVIANE R., SOUZA,ALEX F., MIRANDA,MAYKER L.D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000603005
Resumo: ABSTRACT Leishmaniasis and trypanosomiasis are globally widespread parasitic diseases which have been responsible for high mortality rates. Since drugs available for their treatment are highly hepatotoxic, nephrotoxic and cardiotoxic, adherence to therapy has been affected. Thus, the search for new, more effective and safer drugs for the treatment of these diseases is necessary. Natural products have stood out as an alternative to searching for new bioactive molecules with therapeutic potential. In this study, the chemical composition and antiparasitic activity of the essential oil from Protium ovatum leaves against trypomastigote forms of Trypanosoma cruzi and the promastigote forms of Leishmania amazonensis were evaluated. The essential oil was promising against trypomastigote forms of T. cruzi (IC50= 28.55 μg.mL-1) and L. amazonensis promastigotes (IC50 = 2.28 μg.mL-1). Eighteen chemical constituents were identified by Gas Chromatography coupled to Mass Spectrometry (GC-MS) in the essential oil, whose major constituents were spathulenol (17.6 %), caryophyllene oxide (16.4 %), β-caryophyllene (14.0 %) and myrcene (8.4 %). In addition, the essential oil from P. ovatum leaves had moderate cytotoxicity against LLCMK2 adherent epithelial cell at the concentration range under analysis (CC50 = 150.9 μg.mL-1). It should be highlighted that this is the first report of the chemical composition and anti-Trypanosoma cruzi and anti-Leishmania amazonensis activities of the essential oil from Protium ovatum leaves.
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spelling In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)Trypanosoma cruziLeishmania amazonensisessential oilProtium ovatumparasitic diseasesABSTRACT Leishmaniasis and trypanosomiasis are globally widespread parasitic diseases which have been responsible for high mortality rates. Since drugs available for their treatment are highly hepatotoxic, nephrotoxic and cardiotoxic, adherence to therapy has been affected. Thus, the search for new, more effective and safer drugs for the treatment of these diseases is necessary. Natural products have stood out as an alternative to searching for new bioactive molecules with therapeutic potential. In this study, the chemical composition and antiparasitic activity of the essential oil from Protium ovatum leaves against trypomastigote forms of Trypanosoma cruzi and the promastigote forms of Leishmania amazonensis were evaluated. The essential oil was promising against trypomastigote forms of T. cruzi (IC50= 28.55 μg.mL-1) and L. amazonensis promastigotes (IC50 = 2.28 μg.mL-1). Eighteen chemical constituents were identified by Gas Chromatography coupled to Mass Spectrometry (GC-MS) in the essential oil, whose major constituents were spathulenol (17.6 %), caryophyllene oxide (16.4 %), β-caryophyllene (14.0 %) and myrcene (8.4 %). In addition, the essential oil from P. ovatum leaves had moderate cytotoxicity against LLCMK2 adherent epithelial cell at the concentration range under analysis (CC50 = 150.9 μg.mL-1). It should be highlighted that this is the first report of the chemical composition and anti-Trypanosoma cruzi and anti-Leishmania amazonensis activities of the essential oil from Protium ovatum leaves.Academia Brasileira de Ciências2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000603005Anais da Academia Brasileira de Ciências v.89 n.4 2017reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201720170310info:eu-repo/semantics/openAccessESTEVAM,ELISÂNGELA B.B.DEUS,ISABELLA P.B. DESILVA,VANESSA P. DASILVA,ELIZABETH A.J. DAALVES,CASSIA C.F.ALVES,JOSÉ MILTONCAZAL,CRISTIANE M.MAGALHÃES,LIZANDRA G.PAGOTTI,MARIANA C.ESPERANDIM,VIVIANE R.SOUZA,ALEX F.MIRANDA,MAYKER L.D.eng2017-12-13T00:00:00Zoai:scielo:S0001-37652017000603005Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2017-12-13T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)
title In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)
spellingShingle In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)
ESTEVAM,ELISÂNGELA B.B.
Trypanosoma cruzi
Leishmania amazonensis
essential oil
Protium ovatum
parasitic diseases
title_short In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)
title_full In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)
title_fullStr In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)
title_full_unstemmed In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)
title_sort In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)
author ESTEVAM,ELISÂNGELA B.B.
author_facet ESTEVAM,ELISÂNGELA B.B.
DEUS,ISABELLA P.B. DE
SILVA,VANESSA P. DA
SILVA,ELIZABETH A.J. DA
ALVES,CASSIA C.F.
ALVES,JOSÉ MILTON
CAZAL,CRISTIANE M.
MAGALHÃES,LIZANDRA G.
PAGOTTI,MARIANA C.
ESPERANDIM,VIVIANE R.
SOUZA,ALEX F.
MIRANDA,MAYKER L.D.
author_role author
author2 DEUS,ISABELLA P.B. DE
SILVA,VANESSA P. DA
SILVA,ELIZABETH A.J. DA
ALVES,CASSIA C.F.
ALVES,JOSÉ MILTON
CAZAL,CRISTIANE M.
MAGALHÃES,LIZANDRA G.
PAGOTTI,MARIANA C.
ESPERANDIM,VIVIANE R.
SOUZA,ALEX F.
MIRANDA,MAYKER L.D.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv ESTEVAM,ELISÂNGELA B.B.
DEUS,ISABELLA P.B. DE
SILVA,VANESSA P. DA
SILVA,ELIZABETH A.J. DA
ALVES,CASSIA C.F.
ALVES,JOSÉ MILTON
CAZAL,CRISTIANE M.
MAGALHÃES,LIZANDRA G.
PAGOTTI,MARIANA C.
ESPERANDIM,VIVIANE R.
SOUZA,ALEX F.
MIRANDA,MAYKER L.D.
dc.subject.por.fl_str_mv Trypanosoma cruzi
Leishmania amazonensis
essential oil
Protium ovatum
parasitic diseases
topic Trypanosoma cruzi
Leishmania amazonensis
essential oil
Protium ovatum
parasitic diseases
description ABSTRACT Leishmaniasis and trypanosomiasis are globally widespread parasitic diseases which have been responsible for high mortality rates. Since drugs available for their treatment are highly hepatotoxic, nephrotoxic and cardiotoxic, adherence to therapy has been affected. Thus, the search for new, more effective and safer drugs for the treatment of these diseases is necessary. Natural products have stood out as an alternative to searching for new bioactive molecules with therapeutic potential. In this study, the chemical composition and antiparasitic activity of the essential oil from Protium ovatum leaves against trypomastigote forms of Trypanosoma cruzi and the promastigote forms of Leishmania amazonensis were evaluated. The essential oil was promising against trypomastigote forms of T. cruzi (IC50= 28.55 μg.mL-1) and L. amazonensis promastigotes (IC50 = 2.28 μg.mL-1). Eighteen chemical constituents were identified by Gas Chromatography coupled to Mass Spectrometry (GC-MS) in the essential oil, whose major constituents were spathulenol (17.6 %), caryophyllene oxide (16.4 %), β-caryophyllene (14.0 %) and myrcene (8.4 %). In addition, the essential oil from P. ovatum leaves had moderate cytotoxicity against LLCMK2 adherent epithelial cell at the concentration range under analysis (CC50 = 150.9 μg.mL-1). It should be highlighted that this is the first report of the chemical composition and anti-Trypanosoma cruzi and anti-Leishmania amazonensis activities of the essential oil from Protium ovatum leaves.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000603005
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201720170310
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dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.89 n.4 2017
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