Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves

Detalhes bibliográficos
Autor(a) principal: LUYEN,NGUYEN THI
Data de Publicação: 2018
Outros Autores: DANG,NGUYEN HAI, BINH,PHUNG THI XUAN, HAI,NGUYEN THI, DAT,NGUYEN TIEN
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602881
Resumo: Abstract This paper evaluated the inhibitory effect of 3-O-[β-d-glucopyranosyl-(1→4)-β-d-glucuronopyranosyl] oleanolic acid 28-O-β-d-glucopyranosyl ester (PFS), a major saponin isolated from Polyscias fruticosa leaves, on α-amylase and α-glucosidase, and its potential for reducing the postprandial blood glucose level in mice. In enzyme inhibition assays, PFS strongly inhibited porcine pancreas α-amylase and yeast α-glucosidase. Using the Lineweaver–Burk equation, we found that PFS inhibited porcine pancreas α-amylase in a mixed noncompetitive mode, and yeast α-glucosidase via noncompetitive inhibition. In the sucrose tolerance test, PFS at 100 mg/kg body weight significantly decreased the postprandial blood glucose level in mice fed a high-sucrose diet. These findings suggest that P. fruticosa leaves and their major saponin PFS can be used to prevent and treat diabetes and its complications.
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spelling Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leavesPolyscias fruticosaAraliaceaeα-amylaseα-glucosidasehypoglycemiaAbstract This paper evaluated the inhibitory effect of 3-O-[β-d-glucopyranosyl-(1→4)-β-d-glucuronopyranosyl] oleanolic acid 28-O-β-d-glucopyranosyl ester (PFS), a major saponin isolated from Polyscias fruticosa leaves, on α-amylase and α-glucosidase, and its potential for reducing the postprandial blood glucose level in mice. In enzyme inhibition assays, PFS strongly inhibited porcine pancreas α-amylase and yeast α-glucosidase. Using the Lineweaver–Burk equation, we found that PFS inhibited porcine pancreas α-amylase in a mixed noncompetitive mode, and yeast α-glucosidase via noncompetitive inhibition. In the sucrose tolerance test, PFS at 100 mg/kg body weight significantly decreased the postprandial blood glucose level in mice fed a high-sucrose diet. These findings suggest that P. fruticosa leaves and their major saponin PFS can be used to prevent and treat diabetes and its complications.Academia Brasileira de Ciências2018-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602881Anais da Academia Brasileira de Ciências v.90 n.3 2018reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201820170945info:eu-repo/semantics/openAccessLUYEN,NGUYEN THIDANG,NGUYEN HAIBINH,PHUNG THI XUANHAI,NGUYEN THIDAT,NGUYEN TIENeng2019-11-29T00:00:00Zoai:scielo:S0001-37652018000602881Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-11-29T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves
title Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves
spellingShingle Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves
LUYEN,NGUYEN THI
Polyscias fruticosa
Araliaceae
α-amylase
α-glucosidase
hypoglycemia
title_short Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves
title_full Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves
title_fullStr Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves
title_full_unstemmed Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves
title_sort Hypoglycemic property of triterpenoid saponin PFS isolated from Polyscias fruticosa leaves
author LUYEN,NGUYEN THI
author_facet LUYEN,NGUYEN THI
DANG,NGUYEN HAI
BINH,PHUNG THI XUAN
HAI,NGUYEN THI
DAT,NGUYEN TIEN
author_role author
author2 DANG,NGUYEN HAI
BINH,PHUNG THI XUAN
HAI,NGUYEN THI
DAT,NGUYEN TIEN
author2_role author
author
author
author
dc.contributor.author.fl_str_mv LUYEN,NGUYEN THI
DANG,NGUYEN HAI
BINH,PHUNG THI XUAN
HAI,NGUYEN THI
DAT,NGUYEN TIEN
dc.subject.por.fl_str_mv Polyscias fruticosa
Araliaceae
α-amylase
α-glucosidase
hypoglycemia
topic Polyscias fruticosa
Araliaceae
α-amylase
α-glucosidase
hypoglycemia
description Abstract This paper evaluated the inhibitory effect of 3-O-[β-d-glucopyranosyl-(1→4)-β-d-glucuronopyranosyl] oleanolic acid 28-O-β-d-glucopyranosyl ester (PFS), a major saponin isolated from Polyscias fruticosa leaves, on α-amylase and α-glucosidase, and its potential for reducing the postprandial blood glucose level in mice. In enzyme inhibition assays, PFS strongly inhibited porcine pancreas α-amylase and yeast α-glucosidase. Using the Lineweaver–Burk equation, we found that PFS inhibited porcine pancreas α-amylase in a mixed noncompetitive mode, and yeast α-glucosidase via noncompetitive inhibition. In the sucrose tolerance test, PFS at 100 mg/kg body weight significantly decreased the postprandial blood glucose level in mice fed a high-sucrose diet. These findings suggest that P. fruticosa leaves and their major saponin PFS can be used to prevent and treat diabetes and its complications.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602881
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602881
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201820170945
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.90 n.3 2018
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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