Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers

Detalhes bibliográficos
Autor(a) principal: CORDEIRO,GLÁUCIA
Data de Publicação: 2019
Outros Autores: CUNHA,MARINA S. DA, SILVA,CAROLINA R. DA, JORGE,ISAAC R., DERGAM,JORGE A., FERREIRA,PAULO S.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500860
Resumo: Abstract: Twig girdlers have a short adult life and a long larval stage (up to one year in some species). This fact, together with lack of morphological traits to identify the species from the larval stage, poses obstacles for the taxonomic identification and characterization of the many twig girdlers found in the wild. To solve this matter, Random Amplified Polymorphic DNA (RAPD) molecular markers have been applied to identify some insect species and to determine species boundaries. The aim of this study was to identify three species of Oncideres using the RAPD technique. Adults and larvae of O. saga, O. ocularis and O. ulcerosa were collected in Minas Gerais, Brazil. Two RAPD primers were used (OPA-05 and OPB-13), which produced different electrophoretic profiles that were used to construct a UPGMA phenogram. Three groups identified in the analyses accurately separated the three species, based on individuals in two different stages of development (adults and larvae): I: O. saga, II: O. ulcerosa, and III: O. ocularis. Molecular markers, such as RAPD, are valuable tools that help taxonomists in species identification.
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spelling Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markersGirdled branchesInsect identificationO. ocularisO. sagaO. ulcerosaTwig girdlerAbstract: Twig girdlers have a short adult life and a long larval stage (up to one year in some species). This fact, together with lack of morphological traits to identify the species from the larval stage, poses obstacles for the taxonomic identification and characterization of the many twig girdlers found in the wild. To solve this matter, Random Amplified Polymorphic DNA (RAPD) molecular markers have been applied to identify some insect species and to determine species boundaries. The aim of this study was to identify three species of Oncideres using the RAPD technique. Adults and larvae of O. saga, O. ocularis and O. ulcerosa were collected in Minas Gerais, Brazil. Two RAPD primers were used (OPA-05 and OPB-13), which produced different electrophoretic profiles that were used to construct a UPGMA phenogram. Three groups identified in the analyses accurately separated the three species, based on individuals in two different stages of development (adults and larvae): I: O. saga, II: O. ulcerosa, and III: O. ocularis. Molecular markers, such as RAPD, are valuable tools that help taxonomists in species identification.Academia Brasileira de Ciências2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500860Anais da Academia Brasileira de Ciências v.91 n.3 2019reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201920180209info:eu-repo/semantics/openAccessCORDEIRO,GLÁUCIACUNHA,MARINA S. DASILVA,CAROLINA R. DAJORGE,ISAAC R.DERGAM,JORGE A.FERREIRA,PAULO S.F.eng2019-08-29T00:00:00Zoai:scielo:S0001-37652019000500860Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-08-29T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
title Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
spellingShingle Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
CORDEIRO,GLÁUCIA
Girdled branches
Insect identification
O. ocularis
O. saga
O. ulcerosa
Twig girdler
title_short Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
title_full Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
title_fullStr Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
title_full_unstemmed Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
title_sort Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
author CORDEIRO,GLÁUCIA
author_facet CORDEIRO,GLÁUCIA
CUNHA,MARINA S. DA
SILVA,CAROLINA R. DA
JORGE,ISAAC R.
DERGAM,JORGE A.
FERREIRA,PAULO S.F.
author_role author
author2 CUNHA,MARINA S. DA
SILVA,CAROLINA R. DA
JORGE,ISAAC R.
DERGAM,JORGE A.
FERREIRA,PAULO S.F.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv CORDEIRO,GLÁUCIA
CUNHA,MARINA S. DA
SILVA,CAROLINA R. DA
JORGE,ISAAC R.
DERGAM,JORGE A.
FERREIRA,PAULO S.F.
dc.subject.por.fl_str_mv Girdled branches
Insect identification
O. ocularis
O. saga
O. ulcerosa
Twig girdler
topic Girdled branches
Insect identification
O. ocularis
O. saga
O. ulcerosa
Twig girdler
description Abstract: Twig girdlers have a short adult life and a long larval stage (up to one year in some species). This fact, together with lack of morphological traits to identify the species from the larval stage, poses obstacles for the taxonomic identification and characterization of the many twig girdlers found in the wild. To solve this matter, Random Amplified Polymorphic DNA (RAPD) molecular markers have been applied to identify some insect species and to determine species boundaries. The aim of this study was to identify three species of Oncideres using the RAPD technique. Adults and larvae of O. saga, O. ocularis and O. ulcerosa were collected in Minas Gerais, Brazil. Two RAPD primers were used (OPA-05 and OPB-13), which produced different electrophoretic profiles that were used to construct a UPGMA phenogram. Three groups identified in the analyses accurately separated the three species, based on individuals in two different stages of development (adults and larvae): I: O. saga, II: O. ulcerosa, and III: O. ocularis. Molecular markers, such as RAPD, are valuable tools that help taxonomists in species identification.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500860
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500860
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201920180209
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.91 n.3 2019
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
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instname_str Academia Brasileira de Ciências (ABC)
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institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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