Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100455 |
Resumo: | The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis. |
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Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitisconjunctivitisglycosaminoglycanshyaluronic acidtear filmThe adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis.Academia Brasileira de Ciências2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100455Anais da Academia Brasileira de Ciências v.87 n.1 2015reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201520140122info:eu-repo/semantics/openAccessDREYFUSS,JULIANA L.REGATIERI,CAIO V.COELHO,BRUNOBARBOSA,JOSÉ B.FREITAS,DENISE DENADER,HELENA B.MARTINS,JOÃO R.eng2015-11-16T00:00:00Zoai:scielo:S0001-37652015000100455Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-11-16T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis |
title |
Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis |
spellingShingle |
Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis DREYFUSS,JULIANA L. conjunctivitis glycosaminoglycans hyaluronic acid tear film |
title_short |
Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis |
title_full |
Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis |
title_fullStr |
Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis |
title_full_unstemmed |
Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis |
title_sort |
Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis |
author |
DREYFUSS,JULIANA L. |
author_facet |
DREYFUSS,JULIANA L. REGATIERI,CAIO V. COELHO,BRUNO BARBOSA,JOSÉ B. FREITAS,DENISE DE NADER,HELENA B. MARTINS,JOÃO R. |
author_role |
author |
author2 |
REGATIERI,CAIO V. COELHO,BRUNO BARBOSA,JOSÉ B. FREITAS,DENISE DE NADER,HELENA B. MARTINS,JOÃO R. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
DREYFUSS,JULIANA L. REGATIERI,CAIO V. COELHO,BRUNO BARBOSA,JOSÉ B. FREITAS,DENISE DE NADER,HELENA B. MARTINS,JOÃO R. |
dc.subject.por.fl_str_mv |
conjunctivitis glycosaminoglycans hyaluronic acid tear film |
topic |
conjunctivitis glycosaminoglycans hyaluronic acid tear film |
description |
The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100455 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100455 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201520140122 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.87 n.1 2015 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302861549764608 |