Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR

Detalhes bibliográficos
Autor(a) principal: ORCE,INGRID GEORGINA
Data de Publicação: 2021
Outros Autores: MARTÍNEZ,FABIANA LILIAN, APARICIO,MÓNICA, TORRES,MARÍA JULIA, RAJAL,VERÓNICA BEATRIZ, IRAZUSTA,VERÓNICA PATRICIA
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600901
Resumo: Abstract Random amplified polymorphic DNA-PCR (RAPD-PCR) is a technique successfully used to generate characteristic fingerprints of different bacteria. Bacillus is a genus that includes heterogeneous species, thus a combination of different techniques is essential for their identification. Here we used RAPD-PCR methodology to distinguish among genetically similar strains and to evaluate the genetic diversity of Bacillus species from the Salar del Hombre Muerto, in the Northwest of Argentina. The RAPD-PCR used allowed obtaining different amplification profiles for each Bacillus species and strains. By comparing the fingerprint profiles, we could observe that some of the salt flat isolates showed similar profiles than identified strains. As expected, the bacilli group isolated revealed a wide heterogeneity. RAPD-PCR was found to be a quick and reliable technique to evaluate the diversity of Bacillus strain and was successfully applied to characterize the genetic diversity present in the Salar del Hombre Muerto.
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spelling Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCRRandom Amplified Polymorphism DNA-PCRHalophilesmolecular characterizationbiodiversitypolymorphic Bacillusgenetic characterizationAbstract Random amplified polymorphic DNA-PCR (RAPD-PCR) is a technique successfully used to generate characteristic fingerprints of different bacteria. Bacillus is a genus that includes heterogeneous species, thus a combination of different techniques is essential for their identification. Here we used RAPD-PCR methodology to distinguish among genetically similar strains and to evaluate the genetic diversity of Bacillus species from the Salar del Hombre Muerto, in the Northwest of Argentina. The RAPD-PCR used allowed obtaining different amplification profiles for each Bacillus species and strains. By comparing the fingerprint profiles, we could observe that some of the salt flat isolates showed similar profiles than identified strains. As expected, the bacilli group isolated revealed a wide heterogeneity. RAPD-PCR was found to be a quick and reliable technique to evaluate the diversity of Bacillus strain and was successfully applied to characterize the genetic diversity present in the Salar del Hombre Muerto.Academia Brasileira de Ciências2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600901Anais da Academia Brasileira de Ciências v.93 suppl.3 2021reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202120191430info:eu-repo/semantics/openAccessORCE,INGRID GEORGINAMARTÍNEZ,FABIANA LILIANAPARICIO,MÓNICATORRES,MARÍA JULIARAJAL,VERÓNICA BEATRIZIRAZUSTA,VERÓNICA PATRICIAeng2021-08-05T00:00:00Zoai:scielo:S0001-37652021000600901Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2021-08-05T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
title Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
spellingShingle Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
ORCE,INGRID GEORGINA
Random Amplified Polymorphism DNA-PCR
Halophiles
molecular characterization
biodiversity
polymorphic Bacillus
genetic characterization
title_short Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
title_full Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
title_fullStr Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
title_full_unstemmed Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
title_sort Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
author ORCE,INGRID GEORGINA
author_facet ORCE,INGRID GEORGINA
MARTÍNEZ,FABIANA LILIAN
APARICIO,MÓNICA
TORRES,MARÍA JULIA
RAJAL,VERÓNICA BEATRIZ
IRAZUSTA,VERÓNICA PATRICIA
author_role author
author2 MARTÍNEZ,FABIANA LILIAN
APARICIO,MÓNICA
TORRES,MARÍA JULIA
RAJAL,VERÓNICA BEATRIZ
IRAZUSTA,VERÓNICA PATRICIA
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv ORCE,INGRID GEORGINA
MARTÍNEZ,FABIANA LILIAN
APARICIO,MÓNICA
TORRES,MARÍA JULIA
RAJAL,VERÓNICA BEATRIZ
IRAZUSTA,VERÓNICA PATRICIA
dc.subject.por.fl_str_mv Random Amplified Polymorphism DNA-PCR
Halophiles
molecular characterization
biodiversity
polymorphic Bacillus
genetic characterization
topic Random Amplified Polymorphism DNA-PCR
Halophiles
molecular characterization
biodiversity
polymorphic Bacillus
genetic characterization
description Abstract Random amplified polymorphic DNA-PCR (RAPD-PCR) is a technique successfully used to generate characteristic fingerprints of different bacteria. Bacillus is a genus that includes heterogeneous species, thus a combination of different techniques is essential for their identification. Here we used RAPD-PCR methodology to distinguish among genetically similar strains and to evaluate the genetic diversity of Bacillus species from the Salar del Hombre Muerto, in the Northwest of Argentina. The RAPD-PCR used allowed obtaining different amplification profiles for each Bacillus species and strains. By comparing the fingerprint profiles, we could observe that some of the salt flat isolates showed similar profiles than identified strains. As expected, the bacilli group isolated revealed a wide heterogeneity. RAPD-PCR was found to be a quick and reliable technique to evaluate the diversity of Bacillus strain and was successfully applied to characterize the genetic diversity present in the Salar del Hombre Muerto.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600901
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000600901
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765202120191430
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.93 suppl.3 2021
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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