Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traits
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000501004 |
Resumo: | Abstract Chironomidae are aquatic insects that have become a model in laboratory tests to evaluate toxic effect of different pollutants. The use of chironomids as test organisms depends on standard protocols for the rearing under laboratory conditions and expanding knowledge on its biology. We standardize a culture of C. columbiensis and analyze its life cycle under laboratory conditions. The experiments were carried out with different treatments for water, temperature, and food. As a result, a protocol was proposed for the rearing of C. columbiensis under the following conditions: semi-soft and reconstituted water, shredded paper towels as a substrate, soft aeration, temperature of 22 ± 2°C, fed with 0.3 g TetraMin® twice a week and weekly renewal of 50% of the water. Under these conditions, the duration of the life cycle was 17-31 days, with a continuous growth of 1.16 to 14.05 mm in the larval stage and an average of 530 eggs per oviposition. In comparison with other species in the family, this study indicated that C. columbiensis is a species with a short life cycle, sensible to changing conditions (e.g., temperature and food), wide distribution, so is a good organism for being used in aquatic bioassays. |
id |
ABC-1_d06a76624a48503b63326bee486b6f9c |
---|---|
oai_identifier_str |
oai:scielo:S0001-37652022000501004 |
network_acronym_str |
ABC-1 |
network_name_str |
Anais da Academia Brasileira de Ciências (Online) |
repository_id_str |
|
spelling |
Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traitsChironomus columbiensislaboratory rearinglife cycletest organismAbstract Chironomidae are aquatic insects that have become a model in laboratory tests to evaluate toxic effect of different pollutants. The use of chironomids as test organisms depends on standard protocols for the rearing under laboratory conditions and expanding knowledge on its biology. We standardize a culture of C. columbiensis and analyze its life cycle under laboratory conditions. The experiments were carried out with different treatments for water, temperature, and food. As a result, a protocol was proposed for the rearing of C. columbiensis under the following conditions: semi-soft and reconstituted water, shredded paper towels as a substrate, soft aeration, temperature of 22 ± 2°C, fed with 0.3 g TetraMin® twice a week and weekly renewal of 50% of the water. Under these conditions, the duration of the life cycle was 17-31 days, with a continuous growth of 1.16 to 14.05 mm in the larval stage and an average of 530 eggs per oviposition. In comparison with other species in the family, this study indicated that C. columbiensis is a species with a short life cycle, sensible to changing conditions (e.g., temperature and food), wide distribution, so is a good organism for being used in aquatic bioassays.Academia Brasileira de Ciências2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000501004Anais da Academia Brasileira de Ciências v.94 n.3 2022reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202220210389info:eu-repo/semantics/openAccessMONTAÑO-CAMPAZ,MILTON L.G-DIAS,LUCIMARTORO-RESTREPO,BEATRIZeng2022-06-24T00:00:00Zoai:scielo:S0001-37652022000501004Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2022-06-24T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traits |
title |
Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traits |
spellingShingle |
Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traits MONTAÑO-CAMPAZ,MILTON L. Chironomus columbiensis laboratory rearing life cycle test organism |
title_short |
Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traits |
title_full |
Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traits |
title_fullStr |
Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traits |
title_full_unstemmed |
Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traits |
title_sort |
Chironomus columbiensis (Diptera: Chironomidae) as test organism for aquatic bioassays: Mass rearing and biological traits |
author |
MONTAÑO-CAMPAZ,MILTON L. |
author_facet |
MONTAÑO-CAMPAZ,MILTON L. G-DIAS,LUCIMAR TORO-RESTREPO,BEATRIZ |
author_role |
author |
author2 |
G-DIAS,LUCIMAR TORO-RESTREPO,BEATRIZ |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
MONTAÑO-CAMPAZ,MILTON L. G-DIAS,LUCIMAR TORO-RESTREPO,BEATRIZ |
dc.subject.por.fl_str_mv |
Chironomus columbiensis laboratory rearing life cycle test organism |
topic |
Chironomus columbiensis laboratory rearing life cycle test organism |
description |
Abstract Chironomidae are aquatic insects that have become a model in laboratory tests to evaluate toxic effect of different pollutants. The use of chironomids as test organisms depends on standard protocols for the rearing under laboratory conditions and expanding knowledge on its biology. We standardize a culture of C. columbiensis and analyze its life cycle under laboratory conditions. The experiments were carried out with different treatments for water, temperature, and food. As a result, a protocol was proposed for the rearing of C. columbiensis under the following conditions: semi-soft and reconstituted water, shredded paper towels as a substrate, soft aeration, temperature of 22 ± 2°C, fed with 0.3 g TetraMin® twice a week and weekly renewal of 50% of the water. Under these conditions, the duration of the life cycle was 17-31 days, with a continuous growth of 1.16 to 14.05 mm in the larval stage and an average of 530 eggs per oviposition. In comparison with other species in the family, this study indicated that C. columbiensis is a species with a short life cycle, sensible to changing conditions (e.g., temperature and food), wide distribution, so is a good organism for being used in aquatic bioassays. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000501004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000501004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765202220210389 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.94 n.3 2022 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
_version_ |
1754302872423497728 |