From Gene Targeting to Genome Editing: Transgenic animals applications and beyond

Detalhes bibliográficos
Autor(a) principal: ROCHA-MARTINS,MAURÍCIO
Data de Publicação: 2015
Outros Autores: CAVALHEIRO,GABRIEL R., MATOS-RODRIGUES,GABRIEL E., MARTINS,RODRIGO A.P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301323
Resumo: ABSTRACTGenome modification technologies are powerful tools for molecular biology and related areas. Advances in animal transgenesis and genome editing technologies during the past three decades allowed systematic interrogation of gene function that can help model how the genome influences cellular physiology. Genetic engineering via homologous recombination (HR) has been the standard method to modify genomic sequences. Nevertheless, nuclease-guided genome editing methods that were developed recently, such as ZFN, TALEN and CRISPR/Cas, opened new perspectives for biomedical research. Here, we present a brief historical perspective of genome modification methods, focusing on transgenic mice models. Moreover, we describe how new techniques were discovered and improved, present the paradigm shifts and discuss their limitations and applications for biomedical research as well as possible future directions.
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spelling From Gene Targeting to Genome Editing: Transgenic animals applications and beyondHomologous recombinationDNA repairCre-LoxPZFNTALENCRISPR/Cas9.ABSTRACTGenome modification technologies are powerful tools for molecular biology and related areas. Advances in animal transgenesis and genome editing technologies during the past three decades allowed systematic interrogation of gene function that can help model how the genome influences cellular physiology. Genetic engineering via homologous recombination (HR) has been the standard method to modify genomic sequences. Nevertheless, nuclease-guided genome editing methods that were developed recently, such as ZFN, TALEN and CRISPR/Cas, opened new perspectives for biomedical research. Here, we present a brief historical perspective of genome modification methods, focusing on transgenic mice models. Moreover, we describe how new techniques were discovered and improved, present the paradigm shifts and discuss their limitations and applications for biomedical research as well as possible future directions.Academia Brasileira de Ciências2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301323Anais da Academia Brasileira de Ciências v.87 n.2 suppl.0 2015reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201520140710info:eu-repo/semantics/openAccessROCHA-MARTINS,MAURÍCIOCAVALHEIRO,GABRIEL R.MATOS-RODRIGUES,GABRIEL E.MARTINS,RODRIGO A.P.eng2015-09-17T00:00:00Zoai:scielo:S0001-37652015000301323Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-09-17T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
title From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
spellingShingle From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
ROCHA-MARTINS,MAURÍCIO
Homologous recombination
DNA repair
Cre-LoxP
ZFN
TALEN
CRISPR/Cas9.
title_short From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
title_full From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
title_fullStr From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
title_full_unstemmed From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
title_sort From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
author ROCHA-MARTINS,MAURÍCIO
author_facet ROCHA-MARTINS,MAURÍCIO
CAVALHEIRO,GABRIEL R.
MATOS-RODRIGUES,GABRIEL E.
MARTINS,RODRIGO A.P.
author_role author
author2 CAVALHEIRO,GABRIEL R.
MATOS-RODRIGUES,GABRIEL E.
MARTINS,RODRIGO A.P.
author2_role author
author
author
dc.contributor.author.fl_str_mv ROCHA-MARTINS,MAURÍCIO
CAVALHEIRO,GABRIEL R.
MATOS-RODRIGUES,GABRIEL E.
MARTINS,RODRIGO A.P.
dc.subject.por.fl_str_mv Homologous recombination
DNA repair
Cre-LoxP
ZFN
TALEN
CRISPR/Cas9.
topic Homologous recombination
DNA repair
Cre-LoxP
ZFN
TALEN
CRISPR/Cas9.
description ABSTRACTGenome modification technologies are powerful tools for molecular biology and related areas. Advances in animal transgenesis and genome editing technologies during the past three decades allowed systematic interrogation of gene function that can help model how the genome influences cellular physiology. Genetic engineering via homologous recombination (HR) has been the standard method to modify genomic sequences. Nevertheless, nuclease-guided genome editing methods that were developed recently, such as ZFN, TALEN and CRISPR/Cas, opened new perspectives for biomedical research. Here, we present a brief historical perspective of genome modification methods, focusing on transgenic mice models. Moreover, we describe how new techniques were discovered and improved, present the paradigm shifts and discuss their limitations and applications for biomedical research as well as possible future directions.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301323
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301323
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201520140710
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.87 n.2 suppl.0 2015
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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