From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301323 |
Resumo: | ABSTRACTGenome modification technologies are powerful tools for molecular biology and related areas. Advances in animal transgenesis and genome editing technologies during the past three decades allowed systematic interrogation of gene function that can help model how the genome influences cellular physiology. Genetic engineering via homologous recombination (HR) has been the standard method to modify genomic sequences. Nevertheless, nuclease-guided genome editing methods that were developed recently, such as ZFN, TALEN and CRISPR/Cas, opened new perspectives for biomedical research. Here, we present a brief historical perspective of genome modification methods, focusing on transgenic mice models. Moreover, we describe how new techniques were discovered and improved, present the paradigm shifts and discuss their limitations and applications for biomedical research as well as possible future directions. |
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From Gene Targeting to Genome Editing: Transgenic animals applications and beyondHomologous recombinationDNA repairCre-LoxPZFNTALENCRISPR/Cas9.ABSTRACTGenome modification technologies are powerful tools for molecular biology and related areas. Advances in animal transgenesis and genome editing technologies during the past three decades allowed systematic interrogation of gene function that can help model how the genome influences cellular physiology. Genetic engineering via homologous recombination (HR) has been the standard method to modify genomic sequences. Nevertheless, nuclease-guided genome editing methods that were developed recently, such as ZFN, TALEN and CRISPR/Cas, opened new perspectives for biomedical research. Here, we present a brief historical perspective of genome modification methods, focusing on transgenic mice models. Moreover, we describe how new techniques were discovered and improved, present the paradigm shifts and discuss their limitations and applications for biomedical research as well as possible future directions.Academia Brasileira de Ciências2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301323Anais da Academia Brasileira de Ciências v.87 n.2 suppl.0 2015reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201520140710info:eu-repo/semantics/openAccessROCHA-MARTINS,MAURÍCIOCAVALHEIRO,GABRIEL R.MATOS-RODRIGUES,GABRIEL E.MARTINS,RODRIGO A.P.eng2015-09-17T00:00:00Zoai:scielo:S0001-37652015000301323Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-09-17T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
From Gene Targeting to Genome Editing: Transgenic animals applications and beyond |
title |
From Gene Targeting to Genome Editing: Transgenic animals applications and beyond |
spellingShingle |
From Gene Targeting to Genome Editing: Transgenic animals applications and beyond ROCHA-MARTINS,MAURÍCIO Homologous recombination DNA repair Cre-LoxP ZFN TALEN CRISPR/Cas9. |
title_short |
From Gene Targeting to Genome Editing: Transgenic animals applications and beyond |
title_full |
From Gene Targeting to Genome Editing: Transgenic animals applications and beyond |
title_fullStr |
From Gene Targeting to Genome Editing: Transgenic animals applications and beyond |
title_full_unstemmed |
From Gene Targeting to Genome Editing: Transgenic animals applications and beyond |
title_sort |
From Gene Targeting to Genome Editing: Transgenic animals applications and beyond |
author |
ROCHA-MARTINS,MAURÍCIO |
author_facet |
ROCHA-MARTINS,MAURÍCIO CAVALHEIRO,GABRIEL R. MATOS-RODRIGUES,GABRIEL E. MARTINS,RODRIGO A.P. |
author_role |
author |
author2 |
CAVALHEIRO,GABRIEL R. MATOS-RODRIGUES,GABRIEL E. MARTINS,RODRIGO A.P. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
ROCHA-MARTINS,MAURÍCIO CAVALHEIRO,GABRIEL R. MATOS-RODRIGUES,GABRIEL E. MARTINS,RODRIGO A.P. |
dc.subject.por.fl_str_mv |
Homologous recombination DNA repair Cre-LoxP ZFN TALEN CRISPR/Cas9. |
topic |
Homologous recombination DNA repair Cre-LoxP ZFN TALEN CRISPR/Cas9. |
description |
ABSTRACTGenome modification technologies are powerful tools for molecular biology and related areas. Advances in animal transgenesis and genome editing technologies during the past three decades allowed systematic interrogation of gene function that can help model how the genome influences cellular physiology. Genetic engineering via homologous recombination (HR) has been the standard method to modify genomic sequences. Nevertheless, nuclease-guided genome editing methods that were developed recently, such as ZFN, TALEN and CRISPR/Cas, opened new perspectives for biomedical research. Here, we present a brief historical perspective of genome modification methods, focusing on transgenic mice models. Moreover, we describe how new techniques were discovered and improved, present the paradigm shifts and discuss their limitations and applications for biomedical research as well as possible future directions. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301323 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301323 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201520140710 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.87 n.2 suppl.0 2015 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
_version_ |
1754302860848267264 |