Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000300023 |
Resumo: | This study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC50 value of 12.5 (9.5-16.7) μg/mL] than on glioblastoma SF-295 [IC50 of 25.1 (17.3-36.3) μg/mL] and Sarcoma 180 cells [IC50 of 38.1 (33.5-43.4) μg/mL]. After 72h exposure, flow cytometric and morphological analyses of HL-60-treated cells showed that EEMUS caused decrease in cell number, volume and viability as well as internucleosomal DNA fragmentation in a dose-dependent way, suggesting that the EEMUS triggers apoptotic pathways of cell death. |
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Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plantsantiproliferative potentialNortheastern Brazilian plantsMyracrodruon urundeuvasarcoa 180 tumorseed extractsThis study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC50 value of 12.5 (9.5-16.7) μg/mL] than on glioblastoma SF-295 [IC50 of 25.1 (17.3-36.3) μg/mL] and Sarcoma 180 cells [IC50 of 38.1 (33.5-43.4) μg/mL]. After 72h exposure, flow cytometric and morphological analyses of HL-60-treated cells showed that EEMUS caused decrease in cell number, volume and viability as well as internucleosomal DNA fragmentation in a dose-dependent way, suggesting that the EEMUS triggers apoptotic pathways of cell death.Academia Brasileira de Ciências2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000300023Anais da Academia Brasileira de Ciências v.83 n.3 2011reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/S0001-37652011005000017info:eu-repo/semantics/openAccessFerreira,Paulo Michel P.Farias,Davi F.Viana,Martônio P.Souza,Terezinha M.Vasconcelos,Ilka M.Soares,Bruno M.Pessoa,CláudiaCosta-Lotufo,Letícia V.Moraes,Manoel O.Carvalho,Ana F.U.eng2011-08-15T00:00:00Zoai:scielo:S0001-37652011000300023Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2011-08-15T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants |
title |
Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants |
spellingShingle |
Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants Ferreira,Paulo Michel P. antiproliferative potential Northeastern Brazilian plants Myracrodruon urundeuva sarcoa 180 tumor seed extracts |
title_short |
Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants |
title_full |
Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants |
title_fullStr |
Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants |
title_full_unstemmed |
Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants |
title_sort |
Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants |
author |
Ferreira,Paulo Michel P. |
author_facet |
Ferreira,Paulo Michel P. Farias,Davi F. Viana,Martônio P. Souza,Terezinha M. Vasconcelos,Ilka M. Soares,Bruno M. Pessoa,Cláudia Costa-Lotufo,Letícia V. Moraes,Manoel O. Carvalho,Ana F.U. |
author_role |
author |
author2 |
Farias,Davi F. Viana,Martônio P. Souza,Terezinha M. Vasconcelos,Ilka M. Soares,Bruno M. Pessoa,Cláudia Costa-Lotufo,Letícia V. Moraes,Manoel O. Carvalho,Ana F.U. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Paulo Michel P. Farias,Davi F. Viana,Martônio P. Souza,Terezinha M. Vasconcelos,Ilka M. Soares,Bruno M. Pessoa,Cláudia Costa-Lotufo,Letícia V. Moraes,Manoel O. Carvalho,Ana F.U. |
dc.subject.por.fl_str_mv |
antiproliferative potential Northeastern Brazilian plants Myracrodruon urundeuva sarcoa 180 tumor seed extracts |
topic |
antiproliferative potential Northeastern Brazilian plants Myracrodruon urundeuva sarcoa 180 tumor seed extracts |
description |
This study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC50 value of 12.5 (9.5-16.7) μg/mL] than on glioblastoma SF-295 [IC50 of 25.1 (17.3-36.3) μg/mL] and Sarcoma 180 cells [IC50 of 38.1 (33.5-43.4) μg/mL]. After 72h exposure, flow cytometric and morphological analyses of HL-60-treated cells showed that EEMUS caused decrease in cell number, volume and viability as well as internucleosomal DNA fragmentation in a dose-dependent way, suggesting that the EEMUS triggers apoptotic pathways of cell death. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000300023 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000300023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0001-37652011005000017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.83 n.3 2011 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
_version_ |
1754302858364190720 |