Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors
Autor(a) principal: | |
---|---|
Data de Publicação: | 2005 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000100009 |
Resumo: | Bacillus subtilis and some of its close relatives have a long history of industrial and biotechnological applications. Search for antigen expression systems based on recombinant B. subtilis strains sounds attractive both by the extensive genetic knowledge and the lack of an outer membrane, which simplify the secretion and purification of heterologous proteins. More recently, genetically modified B. subtilis spores have been described as indestructible delivery vehicles for vaccine antigens. Nonetheless both production and delivery of antigens by B. subtilis strains face some inherent obstacles, as unstable gene expression and reduced immunogenicity that, otherwise, can be overcome by already available gene technology approaches. In the present review we present the status of B. subtilis-based vaccine research, either as protein factories or delivery vectors, and discuss some alternatives for a better use of genetically modified strains. |
id |
ABC-1_fbb88980ab62d4b7f564946f1cbf35a6 |
---|---|
oai_identifier_str |
oai:scielo:S0001-37652005000100009 |
network_acronym_str |
ABC-1 |
network_name_str |
Anais da Academia Brasileira de Ciências (Online) |
repository_id_str |
|
spelling |
Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectorsvaccinesBacillus subtilisimmunizationprotein expressionBacillus subtilis and some of its close relatives have a long history of industrial and biotechnological applications. Search for antigen expression systems based on recombinant B. subtilis strains sounds attractive both by the extensive genetic knowledge and the lack of an outer membrane, which simplify the secretion and purification of heterologous proteins. More recently, genetically modified B. subtilis spores have been described as indestructible delivery vehicles for vaccine antigens. Nonetheless both production and delivery of antigens by B. subtilis strains face some inherent obstacles, as unstable gene expression and reduced immunogenicity that, otherwise, can be overcome by already available gene technology approaches. In the present review we present the status of B. subtilis-based vaccine research, either as protein factories or delivery vectors, and discuss some alternatives for a better use of genetically modified strains.Academia Brasileira de Ciências2005-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000100009Anais da Academia Brasileira de Ciências v.77 n.1 2005reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/S0001-37652005000100009info:eu-repo/semantics/openAccessFerreira,Luís C.S.Ferreira,Rita C.C.Schumann,Wolfgangeng2005-02-01T00:00:00Zoai:scielo:S0001-37652005000100009Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2005-02-01T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors |
title |
Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors |
spellingShingle |
Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors Ferreira,Luís C.S. vaccines Bacillus subtilis immunization protein expression |
title_short |
Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors |
title_full |
Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors |
title_fullStr |
Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors |
title_full_unstemmed |
Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors |
title_sort |
Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors |
author |
Ferreira,Luís C.S. |
author_facet |
Ferreira,Luís C.S. Ferreira,Rita C.C. Schumann,Wolfgang |
author_role |
author |
author2 |
Ferreira,Rita C.C. Schumann,Wolfgang |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ferreira,Luís C.S. Ferreira,Rita C.C. Schumann,Wolfgang |
dc.subject.por.fl_str_mv |
vaccines Bacillus subtilis immunization protein expression |
topic |
vaccines Bacillus subtilis immunization protein expression |
description |
Bacillus subtilis and some of its close relatives have a long history of industrial and biotechnological applications. Search for antigen expression systems based on recombinant B. subtilis strains sounds attractive both by the extensive genetic knowledge and the lack of an outer membrane, which simplify the secretion and purification of heterologous proteins. More recently, genetically modified B. subtilis spores have been described as indestructible delivery vehicles for vaccine antigens. Nonetheless both production and delivery of antigens by B. subtilis strains face some inherent obstacles, as unstable gene expression and reduced immunogenicity that, otherwise, can be overcome by already available gene technology approaches. In the present review we present the status of B. subtilis-based vaccine research, either as protein factories or delivery vectors, and discuss some alternatives for a better use of genetically modified strains. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000100009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000100009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0001-37652005000100009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.77 n.1 2005 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
_version_ |
1754302856195735552 |