Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons

Detalhes bibliográficos
Autor(a) principal: Mafra,R.A.
Data de Publicação: 2003
Outros Autores: Leão,R.M., Beirão,P.S.L., Cruz,J.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018
Resumo: A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons.
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spelling Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neuronsCerebellar granule neuronsExcitatory amino-acid transporter type 2Patch-clampGlutamateWhole-cell voltage-clampA glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons.Associação Brasileira de Divulgação Científica2003-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018Brazilian Journal of Medical and Biological Research v.36 n.7 2003reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2003000700018info:eu-repo/semantics/openAccessMafra,R.A.Leão,R.M.Beirão,P.S.L.Cruz,J.S.eng2003-06-26T00:00:00Zoai:scielo:S0100-879X2003000700018Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2003-06-26T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
title Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
spellingShingle Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
Mafra,R.A.
Cerebellar granule neurons
Excitatory amino-acid transporter type 2
Patch-clamp
Glutamate
Whole-cell voltage-clamp
title_short Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
title_full Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
title_fullStr Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
title_full_unstemmed Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
title_sort Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
author Mafra,R.A.
author_facet Mafra,R.A.
Leão,R.M.
Beirão,P.S.L.
Cruz,J.S.
author_role author
author2 Leão,R.M.
Beirão,P.S.L.
Cruz,J.S.
author2_role author
author
author
dc.contributor.author.fl_str_mv Mafra,R.A.
Leão,R.M.
Beirão,P.S.L.
Cruz,J.S.
dc.subject.por.fl_str_mv Cerebellar granule neurons
Excitatory amino-acid transporter type 2
Patch-clamp
Glutamate
Whole-cell voltage-clamp
topic Cerebellar granule neurons
Excitatory amino-acid transporter type 2
Patch-clamp
Glutamate
Whole-cell voltage-clamp
description A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons.
publishDate 2003
dc.date.none.fl_str_mv 2003-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2003000700018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.36 n.7 2003
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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