Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)

Detalhes bibliográficos
Autor(a) principal: Aragão,T.C.F.R.
Data de Publicação: 2000
Outros Autores: Souza,P.A.S., Uchôa,A.F., Costa,I.R., Bloch Jr.,C., Campos,F.A.P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800005
Resumo: We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.
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spelling Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) CactaceaemandacaruCereus jamacarucactin2S albuminsmethionine-rich proteinWe describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.Associação Brasileira de Divulgação Científica2000-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800005Brazilian Journal of Medical and Biological Research v.33 n.8 2000reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2000000800005info:eu-repo/semantics/openAccessAragão,T.C.F.R.Souza,P.A.S.Uchôa,A.F.Costa,I.R.Bloch Jr.,C.Campos,F.A.P.eng2000-07-31T00:00:00Zoai:scielo:S0100-879X2000000800005Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2000-07-31T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
title Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
spellingShingle Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
Aragão,T.C.F.R.
Cactaceae
mandacaru
Cereus jamacaru
cactin
2S albumins
methionine-rich protein
title_short Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
title_full Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
title_fullStr Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
title_full_unstemmed Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
title_sort Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
author Aragão,T.C.F.R.
author_facet Aragão,T.C.F.R.
Souza,P.A.S.
Uchôa,A.F.
Costa,I.R.
Bloch Jr.,C.
Campos,F.A.P.
author_role author
author2 Souza,P.A.S.
Uchôa,A.F.
Costa,I.R.
Bloch Jr.,C.
Campos,F.A.P.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Aragão,T.C.F.R.
Souza,P.A.S.
Uchôa,A.F.
Costa,I.R.
Bloch Jr.,C.
Campos,F.A.P.
dc.subject.por.fl_str_mv Cactaceae
mandacaru
Cereus jamacaru
cactin
2S albumins
methionine-rich protein
topic Cactaceae
mandacaru
Cereus jamacaru
cactin
2S albumins
methionine-rich protein
description We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.
publishDate 2000
dc.date.none.fl_str_mv 2000-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2000000800005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.33 n.8 2000
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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