In vitro cytotoxicity of the LDE: daunorubicin complex in acute myelogenous leukemia blast cells

• Autor(a) principal: Dorlhiac-Llacer,P.E.
• Data de Publicação: 2001
• Outros Autores: Marquezini,M.V., Toffoletto,O., Carneiro,R.C.G., Maranhão,R.C., Chamone,D.A.F.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Medical and Biological Research
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000004
• topic: cytotoxicity; acute myelogenous leukemia; daunorubicin; low-density lipoprotein; LDE complex; RT-PCR

Acute myelogenous leukemia (AML) blast cells show high-affinity degradation of low-density lipoprotein (LDL), suggesting an increased expression of cellular LDL receptors. LDE is a lipid microemulsion easily synthesized in vitro which is known to mimic the metabolic pathway of LDL. We used LDE as a carrier for daunorubicin and assayed the cytotoxicity of the complex using AML blast cells since RT-PCR analysis showed that AML cells express LDL receptor mRNA. The LDE:daunorubicin complex killed 46.7% of blast cells and 20.2% of normal bone marrow cells (P<0.001; Student t-test). Moreover, this complex destroyed AML blast cells as efficiently as free daunorubicin. Thus, LDE might be a suitable carrier of chemotherapeutic agents targeting these drugs to neoplastic cells and protecting normal tissues.