Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300003 |
Resumo: | Restriction fragment length polymorphism (RFLP) was used to examine the extent of mtDNA polymorphism among six strains of rats (Rattus norvegicus) - Wistar, Wistar Munich, Brown Norway, Wistar Kyoto, SHR and SHR-SP. A survey of 26 restriction enzymes has revealed a low level of genetic divergence among strains. The sites of cleavage by EcoRI, NcoI and XmnI were shown to be polymorphic. The use of these three enzymes allows the 6 strains to be classified into 4 haplotypes and identifies specific markers for each one. The percentage of sequence divergence among all pairs of haplotypes ranged from 0.035 to 0.33%, which is the result of a severe population constriction undergone by the strains. These haplotypes are easily demonstrable and therefore RFLP analysis can be employed for genetic monitoring of rats within animal facilities or among different laboratories. |
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Brazilian Journal of Medical and Biological Research |
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Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphismrestriction fragment length polymorphism (RFLP)Rattus norvegicusmtDNAlaboratory ratsgenetic monitoringRestriction fragment length polymorphism (RFLP) was used to examine the extent of mtDNA polymorphism among six strains of rats (Rattus norvegicus) - Wistar, Wistar Munich, Brown Norway, Wistar Kyoto, SHR and SHR-SP. A survey of 26 restriction enzymes has revealed a low level of genetic divergence among strains. The sites of cleavage by EcoRI, NcoI and XmnI were shown to be polymorphic. The use of these three enzymes allows the 6 strains to be classified into 4 haplotypes and identifies specific markers for each one. The percentage of sequence divergence among all pairs of haplotypes ranged from 0.035 to 0.33%, which is the result of a severe population constriction undergone by the strains. These haplotypes are easily demonstrable and therefore RFLP analysis can be employed for genetic monitoring of rats within animal facilities or among different laboratories.Associação Brasileira de Divulgação Científica1999-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300003Brazilian Journal of Medical and Biological Research v.32 n.3 1999reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1999000300003info:eu-repo/semantics/openAccessHilsdorf,A.W.Krieger,J.E.eng1999-03-17T00:00:00Zoai:scielo:S0100-879X1999000300003Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1999-03-17T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism |
title |
Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism |
spellingShingle |
Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism Hilsdorf,A.W. restriction fragment length polymorphism (RFLP) Rattus norvegicus mtDNA laboratory rats genetic monitoring |
title_short |
Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism |
title_full |
Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism |
title_fullStr |
Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism |
title_full_unstemmed |
Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism |
title_sort |
Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism |
author |
Hilsdorf,A.W. |
author_facet |
Hilsdorf,A.W. Krieger,J.E. |
author_role |
author |
author2 |
Krieger,J.E. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Hilsdorf,A.W. Krieger,J.E. |
dc.subject.por.fl_str_mv |
restriction fragment length polymorphism (RFLP) Rattus norvegicus mtDNA laboratory rats genetic monitoring |
topic |
restriction fragment length polymorphism (RFLP) Rattus norvegicus mtDNA laboratory rats genetic monitoring |
description |
Restriction fragment length polymorphism (RFLP) was used to examine the extent of mtDNA polymorphism among six strains of rats (Rattus norvegicus) - Wistar, Wistar Munich, Brown Norway, Wistar Kyoto, SHR and SHR-SP. A survey of 26 restriction enzymes has revealed a low level of genetic divergence among strains. The sites of cleavage by EcoRI, NcoI and XmnI were shown to be polymorphic. The use of these three enzymes allows the 6 strains to be classified into 4 haplotypes and identifies specific markers for each one. The percentage of sequence divergence among all pairs of haplotypes ranged from 0.035 to 0.33%, which is the result of a severe population constriction undergone by the strains. These haplotypes are easily demonstrable and therefore RFLP analysis can be employed for genetic monitoring of rats within animal facilities or among different laboratories. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X1999000300003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.32 n.3 1999 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302929677844480 |