Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12

• Autor(a) principal: Camargo,A.A.
• Data de Publicação: 2001
• Outros Autores: Nunes,D.N., Samaia,H.B., Liu,L., Collins,V.P., Simpson,A.J.G., Dias-Neto,E.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Medical and Biological Research
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000002
• topic: DDX26; DEAD-box RNA helicase; 7p12; ribosome biogenesis

DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evaluated. The predicted amino acid sequence shows a high similarity to a yeast DEAD-box RNA helicase (Dbp9b) involved in ribosome biogenesis. The new helicase maps to 7p12, a region of frequent chromosome amplifications in glioblastomas involving the epidermal growth factor receptor (EGFR) gene. Nevertheless, co-amplification of DDX26 with EGFR was not detected in nine tumors analyzed.