Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats

Detalhes bibliográficos
Autor(a) principal: Samano,M.N.
Data de Publicação: 2009
Outros Autores: Pazetti,R., Prado,C.M., Tibério,I.C., Saldiva,P.H.N., Moreira,L.F.P., Pêgo-Fernandes,P.M., Jatene,F.B., Das-Neves-Pereira,J.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100019
Resumo: Pneumonectomy is associated with high mortality and high rates of complications. Postpneumonectomy pulmonary edema is one of the leading causes of mortality. Little is known about its etiologic factors and its association with the inflammatory process. The purpose of the present study was to evaluate the role of pneumonectomy as a cause of pulmonary edema and its association with gas exchange, inflammation, nitric oxide synthase (NOS) expression and vasoconstriction. Forty-two non-specific pathogen-free Wistar rats were included in the study. Eleven animals died during or after the procedure, 21 were submitted to left pneumonectomy and 10 to sham operation. These animals were sacrificed after 48 or 72 h. Perivascular pulmonary edema was more intense in pneumonectomized rats at 72 h (P = 0.0131). Neutrophil density was lower after pneumonectomy in both groups (P = 0.0168). There was higher immunohistochemical expression of eNOS in the pneumonectomy group (P = 0.0208), but no statistically significant difference in the expression of iNOS. The lumen-wall ratio and pO2/FiO2 ratio did not differ between the operated and sham groups after pneumonectomy. Left pneumonectomy caused perivascular pulmonary edema with no elevation of immunohistochemical expression of iNOS or neutrophil density, suggesting the absence of correlation with the inflammatory process or oxidative stress. The increased expression of eNOS may suggest an intrinsic production of NO without signs of vascular reactivity.
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spelling Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of ratsPneumonectomyPulmonary edemaNitric oxide synthaseAnimal modelsNOSPneumonectomy is associated with high mortality and high rates of complications. Postpneumonectomy pulmonary edema is one of the leading causes of mortality. Little is known about its etiologic factors and its association with the inflammatory process. The purpose of the present study was to evaluate the role of pneumonectomy as a cause of pulmonary edema and its association with gas exchange, inflammation, nitric oxide synthase (NOS) expression and vasoconstriction. Forty-two non-specific pathogen-free Wistar rats were included in the study. Eleven animals died during or after the procedure, 21 were submitted to left pneumonectomy and 10 to sham operation. These animals were sacrificed after 48 or 72 h. Perivascular pulmonary edema was more intense in pneumonectomized rats at 72 h (P = 0.0131). Neutrophil density was lower after pneumonectomy in both groups (P = 0.0168). There was higher immunohistochemical expression of eNOS in the pneumonectomy group (P = 0.0208), but no statistically significant difference in the expression of iNOS. The lumen-wall ratio and pO2/FiO2 ratio did not differ between the operated and sham groups after pneumonectomy. Left pneumonectomy caused perivascular pulmonary edema with no elevation of immunohistochemical expression of iNOS or neutrophil density, suggesting the absence of correlation with the inflammatory process or oxidative stress. The increased expression of eNOS may suggest an intrinsic production of NO without signs of vascular reactivity.Associação Brasileira de Divulgação Científica2009-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100019Brazilian Journal of Medical and Biological Research v.42 n.11 2009reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2009001100019info:eu-repo/semantics/openAccessSamano,M.N.Pazetti,R.Prado,C.M.Tibério,I.C.Saldiva,P.H.N.Moreira,L.F.P.Pêgo-Fernandes,P.M.Jatene,F.B.Das-Neves-Pereira,J.C.eng2009-10-19T00:00:00Zoai:scielo:S0100-879X2009001100019Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2009-10-19T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
title Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
spellingShingle Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
Samano,M.N.
Pneumonectomy
Pulmonary edema
Nitric oxide synthase
Animal models
NOS
title_short Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
title_full Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
title_fullStr Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
title_full_unstemmed Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
title_sort Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
author Samano,M.N.
author_facet Samano,M.N.
Pazetti,R.
Prado,C.M.
Tibério,I.C.
Saldiva,P.H.N.
Moreira,L.F.P.
Pêgo-Fernandes,P.M.
Jatene,F.B.
Das-Neves-Pereira,J.C.
author_role author
author2 Pazetti,R.
Prado,C.M.
Tibério,I.C.
Saldiva,P.H.N.
Moreira,L.F.P.
Pêgo-Fernandes,P.M.
Jatene,F.B.
Das-Neves-Pereira,J.C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Samano,M.N.
Pazetti,R.
Prado,C.M.
Tibério,I.C.
Saldiva,P.H.N.
Moreira,L.F.P.
Pêgo-Fernandes,P.M.
Jatene,F.B.
Das-Neves-Pereira,J.C.
dc.subject.por.fl_str_mv Pneumonectomy
Pulmonary edema
Nitric oxide synthase
Animal models
NOS
topic Pneumonectomy
Pulmonary edema
Nitric oxide synthase
Animal models
NOS
description Pneumonectomy is associated with high mortality and high rates of complications. Postpneumonectomy pulmonary edema is one of the leading causes of mortality. Little is known about its etiologic factors and its association with the inflammatory process. The purpose of the present study was to evaluate the role of pneumonectomy as a cause of pulmonary edema and its association with gas exchange, inflammation, nitric oxide synthase (NOS) expression and vasoconstriction. Forty-two non-specific pathogen-free Wistar rats were included in the study. Eleven animals died during or after the procedure, 21 were submitted to left pneumonectomy and 10 to sham operation. These animals were sacrificed after 48 or 72 h. Perivascular pulmonary edema was more intense in pneumonectomized rats at 72 h (P = 0.0131). Neutrophil density was lower after pneumonectomy in both groups (P = 0.0168). There was higher immunohistochemical expression of eNOS in the pneumonectomy group (P = 0.0208), but no statistically significant difference in the expression of iNOS. The lumen-wall ratio and pO2/FiO2 ratio did not differ between the operated and sham groups after pneumonectomy. Left pneumonectomy caused perivascular pulmonary edema with no elevation of immunohistochemical expression of iNOS or neutrophil density, suggesting the absence of correlation with the inflammatory process or oxidative stress. The increased expression of eNOS may suggest an intrinsic production of NO without signs of vascular reactivity.
publishDate 2009
dc.date.none.fl_str_mv 2009-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2009001100019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.42 n.11 2009
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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