Hunting for differentially expressed genes
Autor(a) principal: | |
---|---|
Data de Publicação: | 1999 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700012 |
Resumo: | Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. Recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. Here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field. |
id |
ABDC-1_39bb8677191eca2bcb4ac895e6b54aef |
---|---|
oai_identifier_str |
oai:scielo:S0100-879X1999000700012 |
network_acronym_str |
ABDC-1 |
network_name_str |
Brazilian Journal of Medical and Biological Research |
repository_id_str |
|
spelling |
Hunting for differentially expressed genesdifferential gene expressiondifferential hybridizationdifferential display/DDRTsubtraction hybridizationsuppressive PCRDNA microarrays/chipsDifferentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. Recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. Here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field.Associação Brasileira de Divulgação Científica1999-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700012Brazilian Journal of Medical and Biological Research v.32 n.7 1999reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1999000700012info:eu-repo/semantics/openAccessVedoy,C.G.Bengtson,M.H.Sogayar,M.C.eng1999-06-25T00:00:00Zoai:scielo:S0100-879X1999000700012Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1999-06-25T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Hunting for differentially expressed genes |
title |
Hunting for differentially expressed genes |
spellingShingle |
Hunting for differentially expressed genes Vedoy,C.G. differential gene expression differential hybridization differential display/DDRT subtraction hybridization suppressive PCR DNA microarrays/chips |
title_short |
Hunting for differentially expressed genes |
title_full |
Hunting for differentially expressed genes |
title_fullStr |
Hunting for differentially expressed genes |
title_full_unstemmed |
Hunting for differentially expressed genes |
title_sort |
Hunting for differentially expressed genes |
author |
Vedoy,C.G. |
author_facet |
Vedoy,C.G. Bengtson,M.H. Sogayar,M.C. |
author_role |
author |
author2 |
Bengtson,M.H. Sogayar,M.C. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Vedoy,C.G. Bengtson,M.H. Sogayar,M.C. |
dc.subject.por.fl_str_mv |
differential gene expression differential hybridization differential display/DDRT subtraction hybridization suppressive PCR DNA microarrays/chips |
topic |
differential gene expression differential hybridization differential display/DDRT subtraction hybridization suppressive PCR DNA microarrays/chips |
description |
Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. Recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. Here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X1999000700012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.32 n.7 1999 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302930013388800 |