The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100015 |
Resumo: | In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein phosphorylation, revealed by immunocytochemistry, and sites of actin assembly, revealed by the use of labeled phaloidin. The results obtained show that proteins localized in the interaction sites are phosphorylated. The process of formation of the parasitophorous vacuole was monitored by labeling the host cell surface with fluorescent probes for lipids (PKH26), proteins (DTAF) and sialic acid (FITC-thiosemicarbazide) before interaction with the parasites. Evidence was obtained indicating transfer of components of the host cell surface to the parasite surface in the beginning of the interaction process. We also analyzed the distribution of cytoskeletal structures (microtubules and microfilaments visualized with specific antibodies), mitochondria (visualized with rhodamine 123), the Golgi complex (visualized with C6-NBD-ceramide) and the endoplasmic reticulum (visualized with anti-reticulin antibodies and DIOC6) during the evolution of intracellular parasitism. The results obtained show that some, but not all, structures change their position during evolution of the intracellular parasitism. |
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Brazilian Journal of Medical and Biological Research |
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The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interactionconfocal laser scanning microscopyhost cell-parasite interactionTrypanosoma cruziToxoplasma gondiiIn this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein phosphorylation, revealed by immunocytochemistry, and sites of actin assembly, revealed by the use of labeled phaloidin. The results obtained show that proteins localized in the interaction sites are phosphorylated. The process of formation of the parasitophorous vacuole was monitored by labeling the host cell surface with fluorescent probes for lipids (PKH26), proteins (DTAF) and sialic acid (FITC-thiosemicarbazide) before interaction with the parasites. Evidence was obtained indicating transfer of components of the host cell surface to the parasite surface in the beginning of the interaction process. We also analyzed the distribution of cytoskeletal structures (microtubules and microfilaments visualized with specific antibodies), mitochondria (visualized with rhodamine 123), the Golgi complex (visualized with C6-NBD-ceramide) and the endoplasmic reticulum (visualized with anti-reticulin antibodies and DIOC6) during the evolution of intracellular parasitism. The results obtained show that some, but not all, structures change their position during evolution of the intracellular parasitism.Associação Brasileira de Divulgação Científica1998-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100015Brazilian Journal of Medical and Biological Research v.31 n.11 1998reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1998001100015info:eu-repo/semantics/openAccessde-Souza,W.de-Carvalho,T.U.de-Melo,E.T.Soares,C.P.Coimbra,E.S.Rosestolato,C.T.Ferreira,S.R.Vieira,M.eng1998-10-20T00:00:00Zoai:scielo:S0100-879X1998001100015Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1998-10-20T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction |
title |
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction |
spellingShingle |
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction de-Souza,W. confocal laser scanning microscopy host cell-parasite interaction Trypanosoma cruzi Toxoplasma gondii |
title_short |
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction |
title_full |
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction |
title_fullStr |
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction |
title_full_unstemmed |
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction |
title_sort |
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction |
author |
de-Souza,W. |
author_facet |
de-Souza,W. de-Carvalho,T.U. de-Melo,E.T. Soares,C.P. Coimbra,E.S. Rosestolato,C.T. Ferreira,S.R. Vieira,M. |
author_role |
author |
author2 |
de-Carvalho,T.U. de-Melo,E.T. Soares,C.P. Coimbra,E.S. Rosestolato,C.T. Ferreira,S.R. Vieira,M. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
de-Souza,W. de-Carvalho,T.U. de-Melo,E.T. Soares,C.P. Coimbra,E.S. Rosestolato,C.T. Ferreira,S.R. Vieira,M. |
dc.subject.por.fl_str_mv |
confocal laser scanning microscopy host cell-parasite interaction Trypanosoma cruzi Toxoplasma gondii |
topic |
confocal laser scanning microscopy host cell-parasite interaction Trypanosoma cruzi Toxoplasma gondii |
description |
In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein phosphorylation, revealed by immunocytochemistry, and sites of actin assembly, revealed by the use of labeled phaloidin. The results obtained show that proteins localized in the interaction sites are phosphorylated. The process of formation of the parasitophorous vacuole was monitored by labeling the host cell surface with fluorescent probes for lipids (PKH26), proteins (DTAF) and sialic acid (FITC-thiosemicarbazide) before interaction with the parasites. Evidence was obtained indicating transfer of components of the host cell surface to the parasite surface in the beginning of the interaction process. We also analyzed the distribution of cytoskeletal structures (microtubules and microfilaments visualized with specific antibodies), mitochondria (visualized with rhodamine 123), the Golgi complex (visualized with C6-NBD-ceramide) and the endoplasmic reticulum (visualized with anti-reticulin antibodies and DIOC6) during the evolution of intracellular parasitism. The results obtained show that some, but not all, structures change their position during evolution of the intracellular parasitism. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X1998001100015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.31 n.11 1998 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302929617027072 |