Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200164 |
Resumo: | Myosin Va functions as a processive, actin-based motor molecule highly enriched in the nervous system, which transports and/or tethers organelles, vesicles, and mRNA and protein translation machinery. Mutation of myosin Va leads to Griscelli disease that is associated with severe neurological deficits and a short life span. Despite playing a critical role in development, the expression of myosin Va in the central nervous system throughout the human life span has not been reported. To address this issue, the cerebellar expression of myosin Va from newborns to elderly humans was studied by immunohistochemistry using an affinity-purified anti-myosin Va antibody. Myosin Va was expressed at all ages from the 10th postnatal day to the 98th year of life, in molecular, Purkinje and granular cerebellar layers. Cerebellar myosin Va expression did not differ essentially in localization or intensity from childhood to old age, except during the postnatal developmental period. Structures resembling granules and climbing fibers in Purkinje cells were deeply stained. In dentate neurons, long processes were deeply stained by anti-myosin Va, as were punctate nuclear structures. During the first postnatal year, myosin Va was differentially expressed in the external granular layer (EGL). In the EGL, proliferating prospective granule cells were not stained by anti-myosin Va antibody. In contrast, premigratory granule cells in the EGL stained moderately. Granule cells exhibiting a migratory profile in the molecular layer were also moderately stained. In conclusion, neuronal myosin Va is developmentally regulated, and appears to be required for cerebellar function from early postnatal life to senescence. |
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Brazilian Journal of Medical and Biological Research |
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Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old ageMyosin VaHuman cerebellumPostnatal developmentAgingImmunohistochemical expressionMyosin Va functions as a processive, actin-based motor molecule highly enriched in the nervous system, which transports and/or tethers organelles, vesicles, and mRNA and protein translation machinery. Mutation of myosin Va leads to Griscelli disease that is associated with severe neurological deficits and a short life span. Despite playing a critical role in development, the expression of myosin Va in the central nervous system throughout the human life span has not been reported. To address this issue, the cerebellar expression of myosin Va from newborns to elderly humans was studied by immunohistochemistry using an affinity-purified anti-myosin Va antibody. Myosin Va was expressed at all ages from the 10th postnatal day to the 98th year of life, in molecular, Purkinje and granular cerebellar layers. Cerebellar myosin Va expression did not differ essentially in localization or intensity from childhood to old age, except during the postnatal developmental period. Structures resembling granules and climbing fibers in Purkinje cells were deeply stained. In dentate neurons, long processes were deeply stained by anti-myosin Va, as were punctate nuclear structures. During the first postnatal year, myosin Va was differentially expressed in the external granular layer (EGL). In the EGL, proliferating prospective granule cells were not stained by anti-myosin Va antibody. In contrast, premigratory granule cells in the EGL stained moderately. Granule cells exhibiting a migratory profile in the molecular layer were also moderately stained. In conclusion, neuronal myosin Va is developmentally regulated, and appears to be required for cerebellar function from early postnatal life to senescence.Associação Brasileira de Divulgação Científica2013-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200164Brazilian Journal of Medical and Biological Research v.46 n.2 2013reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431X20122627info:eu-repo/semantics/openAccessSouza,C.C.R.Dombroski,T.C.D.Machado,H.R.Oliveira,R.S.Rocha,L.B.Rodrigues,A.R.A.Neder,L.Chimelli,L.Corrêa,V.M.A.Larson,R.E.Martins,A.R.eng2015-10-08T00:00:00Zoai:scielo:S0100-879X2013000200164Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2015-10-08T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age |
title |
Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age |
spellingShingle |
Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age Souza,C.C.R. Myosin Va Human cerebellum Postnatal development Aging Immunohistochemical expression |
title_short |
Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age |
title_full |
Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age |
title_fullStr |
Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age |
title_full_unstemmed |
Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age |
title_sort |
Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age |
author |
Souza,C.C.R. |
author_facet |
Souza,C.C.R. Dombroski,T.C.D. Machado,H.R. Oliveira,R.S. Rocha,L.B. Rodrigues,A.R.A. Neder,L. Chimelli,L. Corrêa,V.M.A. Larson,R.E. Martins,A.R. |
author_role |
author |
author2 |
Dombroski,T.C.D. Machado,H.R. Oliveira,R.S. Rocha,L.B. Rodrigues,A.R.A. Neder,L. Chimelli,L. Corrêa,V.M.A. Larson,R.E. Martins,A.R. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza,C.C.R. Dombroski,T.C.D. Machado,H.R. Oliveira,R.S. Rocha,L.B. Rodrigues,A.R.A. Neder,L. Chimelli,L. Corrêa,V.M.A. Larson,R.E. Martins,A.R. |
dc.subject.por.fl_str_mv |
Myosin Va Human cerebellum Postnatal development Aging Immunohistochemical expression |
topic |
Myosin Va Human cerebellum Postnatal development Aging Immunohistochemical expression |
description |
Myosin Va functions as a processive, actin-based motor molecule highly enriched in the nervous system, which transports and/or tethers organelles, vesicles, and mRNA and protein translation machinery. Mutation of myosin Va leads to Griscelli disease that is associated with severe neurological deficits and a short life span. Despite playing a critical role in development, the expression of myosin Va in the central nervous system throughout the human life span has not been reported. To address this issue, the cerebellar expression of myosin Va from newborns to elderly humans was studied by immunohistochemistry using an affinity-purified anti-myosin Va antibody. Myosin Va was expressed at all ages from the 10th postnatal day to the 98th year of life, in molecular, Purkinje and granular cerebellar layers. Cerebellar myosin Va expression did not differ essentially in localization or intensity from childhood to old age, except during the postnatal developmental period. Structures resembling granules and climbing fibers in Purkinje cells were deeply stained. In dentate neurons, long processes were deeply stained by anti-myosin Va, as were punctate nuclear structures. During the first postnatal year, myosin Va was differentially expressed in the external granular layer (EGL). In the EGL, proliferating prospective granule cells were not stained by anti-myosin Va antibody. In contrast, premigratory granule cells in the EGL stained moderately. Granule cells exhibiting a migratory profile in the molecular layer were also moderately stained. In conclusion, neuronal myosin Va is developmentally regulated, and appears to be required for cerebellar function from early postnatal life to senescence. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200164 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200164 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431X20122627 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.46 n.2 2013 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
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1754302942012243968 |