CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell

Detalhes bibliográficos
Autor(a) principal: Aguiar,D.P.
Data de Publicação: 2011
Outros Autores: Coelho-Aguiar,J.M., Abreu,J.G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004
Resumo: Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells.
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spelling CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cellTeratocarcinomaAdhesionCondensationCCN familyCCN2/CTGFExtracellular matrixConnective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells.Associação Brasileira de Divulgação Científica2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004Brazilian Journal of Medical and Biological Research v.44 n.3 2011reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2011007500019info:eu-repo/semantics/openAccessAguiar,D.P.Coelho-Aguiar,J.M.Abreu,J.G.eng2015-03-20T00:00:00Zoai:scielo:S0100-879X2011000300004Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2015-03-20T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
title CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
spellingShingle CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
Aguiar,D.P.
Teratocarcinoma
Adhesion
Condensation
CCN family
CCN2/CTGF
Extracellular matrix
title_short CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
title_full CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
title_fullStr CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
title_full_unstemmed CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
title_sort CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
author Aguiar,D.P.
author_facet Aguiar,D.P.
Coelho-Aguiar,J.M.
Abreu,J.G.
author_role author
author2 Coelho-Aguiar,J.M.
Abreu,J.G.
author2_role author
author
dc.contributor.author.fl_str_mv Aguiar,D.P.
Coelho-Aguiar,J.M.
Abreu,J.G.
dc.subject.por.fl_str_mv Teratocarcinoma
Adhesion
Condensation
CCN family
CCN2/CTGF
Extracellular matrix
topic Teratocarcinoma
Adhesion
Condensation
CCN family
CCN2/CTGF
Extracellular matrix
description Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2011007500019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.44 n.3 2011
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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