CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004 |
Resumo: | Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells. |
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Brazilian Journal of Medical and Biological Research |
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CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cellTeratocarcinomaAdhesionCondensationCCN familyCCN2/CTGFExtracellular matrixConnective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells.Associação Brasileira de Divulgação Científica2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004Brazilian Journal of Medical and Biological Research v.44 n.3 2011reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2011007500019info:eu-repo/semantics/openAccessAguiar,D.P.Coelho-Aguiar,J.M.Abreu,J.G.eng2015-03-20T00:00:00Zoai:scielo:S0100-879X2011000300004Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2015-03-20T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell |
title |
CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell |
spellingShingle |
CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell Aguiar,D.P. Teratocarcinoma Adhesion Condensation CCN family CCN2/CTGF Extracellular matrix |
title_short |
CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell |
title_full |
CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell |
title_fullStr |
CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell |
title_full_unstemmed |
CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell |
title_sort |
CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell |
author |
Aguiar,D.P. |
author_facet |
Aguiar,D.P. Coelho-Aguiar,J.M. Abreu,J.G. |
author_role |
author |
author2 |
Coelho-Aguiar,J.M. Abreu,J.G. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Aguiar,D.P. Coelho-Aguiar,J.M. Abreu,J.G. |
dc.subject.por.fl_str_mv |
Teratocarcinoma Adhesion Condensation CCN family CCN2/CTGF Extracellular matrix |
topic |
Teratocarcinoma Adhesion Condensation CCN family CCN2/CTGF Extracellular matrix |
description |
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2011007500019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.44 n.3 2011 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302939931869184 |