Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)

Detalhes bibliográficos
Autor(a) principal: Uchoa,A.F.
Data de Publicação: 1998
Outros Autores: Souza,P.A.S., Zarate,R.M.L., Gomes-Filho,E., Campos,F.A.P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600005
Resumo: We describe here the isolation and characterization of a major albumin from the seeds of Opuntia ficus-indica (Cactaceae). This protein has a molecular mass of 6.5 kDa and was isolated by a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of this protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The N-terminal amino acid sequence of this protein is Asp-Pro-Tyr-Trp-Glu-Gln-Arg.
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spelling Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)Cactaceaeprickly-pearOpuntia ficus-indicaseed proteins2S albuminsWe describe here the isolation and characterization of a major albumin from the seeds of Opuntia ficus-indica (Cactaceae). This protein has a molecular mass of 6.5 kDa and was isolated by a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of this protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The N-terminal amino acid sequence of this protein is Asp-Pro-Tyr-Trp-Glu-Gln-Arg.Associação Brasileira de Divulgação Científica1998-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600005Brazilian Journal of Medical and Biological Research v.31 n.6 1998reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1998000600005info:eu-repo/semantics/openAccessUchoa,A.F.Souza,P.A.S.Zarate,R.M.L.Gomes-Filho,E.Campos,F.A.P.eng1998-10-06T00:00:00Zoai:scielo:S0100-879X1998000600005Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1998-10-06T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
title Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
spellingShingle Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
Uchoa,A.F.
Cactaceae
prickly-pear
Opuntia ficus-indica
seed proteins
2S albumins
title_short Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
title_full Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
title_fullStr Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
title_full_unstemmed Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
title_sort Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
author Uchoa,A.F.
author_facet Uchoa,A.F.
Souza,P.A.S.
Zarate,R.M.L.
Gomes-Filho,E.
Campos,F.A.P.
author_role author
author2 Souza,P.A.S.
Zarate,R.M.L.
Gomes-Filho,E.
Campos,F.A.P.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Uchoa,A.F.
Souza,P.A.S.
Zarate,R.M.L.
Gomes-Filho,E.
Campos,F.A.P.
dc.subject.por.fl_str_mv Cactaceae
prickly-pear
Opuntia ficus-indica
seed proteins
2S albumins
topic Cactaceae
prickly-pear
Opuntia ficus-indica
seed proteins
2S albumins
description We describe here the isolation and characterization of a major albumin from the seeds of Opuntia ficus-indica (Cactaceae). This protein has a molecular mass of 6.5 kDa and was isolated by a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of this protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The N-terminal amino acid sequence of this protein is Asp-Pro-Tyr-Trp-Glu-Gln-Arg.
publishDate 1998
dc.date.none.fl_str_mv 1998-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X1998000600005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.31 n.6 1998
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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