Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800005 |
Resumo: | Proteoglycans are abundant in the developing brain and there is much circumstantial evidence for their roles in directional neuronal movements such as cell body migration and axonal growth. We have developed an in vitro model of astrocyte cultures of the lateral and medial sectors of the embryonic mouse midbrain, that differ in their ability to support neuritic growth of young midbrain neurons, and we have searched for the role of interactive proteins and proteoglycans in this model. Neurite production in co-cultures reveals that, irrespective of the previous location of neurons in the midbrain, medial astrocytes exert an inhibitory or nonpermissive effect on neuritic growth that is correlated to a higher content of both heparan and chondroitin sulfates (HS and CS). Treatment of astrocytes with chondroitinase ABC revealed a growth-promoting effect of CS on lateral glia but treatment with exogenous CS-4 indicated a U-shaped dose-response curve for CS. In contrast, the growth-inhibitory action of medial astrocytes was reversed by exogenous CS-4. Treatment of astrocytes with heparitinase indicated that the growth-inhibitory action of medial astrocytes may depend heavily on HS by an as yet unknown mechanism. The results are discussed in terms of available knowledge on the binding of HS proteoglycans to interactive proteins, with emphasis on the importance of unraveling the physiological functions of glial glycoconjugates for a better understanding of neuron-glial interactions. |
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Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrainAstrocytesAxonal growthChondroitin sulfateHeparan sulfateMidlineMidbrainProteoglycans are abundant in the developing brain and there is much circumstantial evidence for their roles in directional neuronal movements such as cell body migration and axonal growth. We have developed an in vitro model of astrocyte cultures of the lateral and medial sectors of the embryonic mouse midbrain, that differ in their ability to support neuritic growth of young midbrain neurons, and we have searched for the role of interactive proteins and proteoglycans in this model. Neurite production in co-cultures reveals that, irrespective of the previous location of neurons in the midbrain, medial astrocytes exert an inhibitory or nonpermissive effect on neuritic growth that is correlated to a higher content of both heparan and chondroitin sulfates (HS and CS). Treatment of astrocytes with chondroitinase ABC revealed a growth-promoting effect of CS on lateral glia but treatment with exogenous CS-4 indicated a U-shaped dose-response curve for CS. In contrast, the growth-inhibitory action of medial astrocytes was reversed by exogenous CS-4. Treatment of astrocytes with heparitinase indicated that the growth-inhibitory action of medial astrocytes may depend heavily on HS by an as yet unknown mechanism. The results are discussed in terms of available knowledge on the binding of HS proteoglycans to interactive proteins, with emphasis on the importance of unraveling the physiological functions of glial glycoconjugates for a better understanding of neuron-glial interactions.Associação Brasileira de Divulgação Científica2003-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800005Brazilian Journal of Medical and Biological Research v.36 n.8 2003reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2003000800005info:eu-repo/semantics/openAccessCavalcante,L.A.Garcia-Abreu,J.Mendes,F.A.Moura Neto,V.Silva,L.C.F.Onofre,G.Weissmüller,G.Carvalho,S.L.eng2003-07-23T00:00:00Zoai:scielo:S0100-879X2003000800005Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2003-07-23T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain |
title |
Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain |
spellingShingle |
Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain Cavalcante,L.A. Astrocytes Axonal growth Chondroitin sulfate Heparan sulfate Midline Midbrain |
title_short |
Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain |
title_full |
Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain |
title_fullStr |
Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain |
title_full_unstemmed |
Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain |
title_sort |
Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain |
author |
Cavalcante,L.A. |
author_facet |
Cavalcante,L.A. Garcia-Abreu,J. Mendes,F.A. Moura Neto,V. Silva,L.C.F. Onofre,G. Weissmüller,G. Carvalho,S.L. |
author_role |
author |
author2 |
Garcia-Abreu,J. Mendes,F.A. Moura Neto,V. Silva,L.C.F. Onofre,G. Weissmüller,G. Carvalho,S.L. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Cavalcante,L.A. Garcia-Abreu,J. Mendes,F.A. Moura Neto,V. Silva,L.C.F. Onofre,G. Weissmüller,G. Carvalho,S.L. |
dc.subject.por.fl_str_mv |
Astrocytes Axonal growth Chondroitin sulfate Heparan sulfate Midline Midbrain |
topic |
Astrocytes Axonal growth Chondroitin sulfate Heparan sulfate Midline Midbrain |
description |
Proteoglycans are abundant in the developing brain and there is much circumstantial evidence for their roles in directional neuronal movements such as cell body migration and axonal growth. We have developed an in vitro model of astrocyte cultures of the lateral and medial sectors of the embryonic mouse midbrain, that differ in their ability to support neuritic growth of young midbrain neurons, and we have searched for the role of interactive proteins and proteoglycans in this model. Neurite production in co-cultures reveals that, irrespective of the previous location of neurons in the midbrain, medial astrocytes exert an inhibitory or nonpermissive effect on neuritic growth that is correlated to a higher content of both heparan and chondroitin sulfates (HS and CS). Treatment of astrocytes with chondroitinase ABC revealed a growth-promoting effect of CS on lateral glia but treatment with exogenous CS-4 indicated a U-shaped dose-response curve for CS. In contrast, the growth-inhibitory action of medial astrocytes was reversed by exogenous CS-4. Treatment of astrocytes with heparitinase indicated that the growth-inhibitory action of medial astrocytes may depend heavily on HS by an as yet unknown mechanism. The results are discussed in terms of available knowledge on the binding of HS proteoglycans to interactive proteins, with emphasis on the importance of unraveling the physiological functions of glial glycoconjugates for a better understanding of neuron-glial interactions. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2003000800005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.36 n.8 2003 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302932462862336 |