Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300013 |
Resumo: | In this study, we report the characterization of a strain of Enterococcus faecium vanA, which grows only in the presence of vancomycin (VDEfm-UEL). The bacterium was isolated from the feces of a female patient who had undergone surgical treatment of Reinke’s edema and was receiving intravenous vancomycin therapy for infection with methicillin/oxacillin-resistant Staphylococcus aureus, a postoperative complication. Antimicrobial dependence was further confirmed by the vancomycin E-test. VDEfm-UEL was also shown to be resistant to ampicillin, ciprofloxacin, chloramphenicol, erythromycin, levofloxacin, penicillin, rifampicin, and teicoplanin. The putative virulence genes efaA, gelE and esp were detected by PCR. The ddl gene from VDEfm-UEL was cloned and sequenced. Vancomycin dependence seems to be associated with the insertion of a nucleotide in that sequence, which results in a frame-shift mutation, introducing a premature stop codon. This is the first report of vancomycin-dependent E. faecium isolation in a university hospital in Brazil. |
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Brazilian Journal of Medical and Biological Research |
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Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in BrazilEnterococcus faeciumVancomycinDependencevanA genotypeVirulence factorsIn this study, we report the characterization of a strain of Enterococcus faecium vanA, which grows only in the presence of vancomycin (VDEfm-UEL). The bacterium was isolated from the feces of a female patient who had undergone surgical treatment of Reinke’s edema and was receiving intravenous vancomycin therapy for infection with methicillin/oxacillin-resistant Staphylococcus aureus, a postoperative complication. Antimicrobial dependence was further confirmed by the vancomycin E-test. VDEfm-UEL was also shown to be resistant to ampicillin, ciprofloxacin, chloramphenicol, erythromycin, levofloxacin, penicillin, rifampicin, and teicoplanin. The putative virulence genes efaA, gelE and esp were detected by PCR. The ddl gene from VDEfm-UEL was cloned and sequenced. Vancomycin dependence seems to be associated with the insertion of a nucleotide in that sequence, which results in a frame-shift mutation, introducing a premature stop codon. This is the first report of vancomycin-dependent E. faecium isolation in a university hospital in Brazil.Associação Brasileira de Divulgação Científica2011-03-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300013Brazilian Journal of Medical and Biological Research v.44 n.3 2011reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2011007500006info:eu-repo/semantics/openAccessKerbauy,G.Perugini,M.R.E.Yamauchi,L.M.Yamada-Ogatta,S.F.eng2015-03-20T00:00:00Zoai:scielo:S0100-879X2011000300013Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2015-03-20T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil |
title |
Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil |
spellingShingle |
Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil Kerbauy,G. Enterococcus faecium Vancomycin Dependence vanA genotype Virulence factors |
title_short |
Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil |
title_full |
Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil |
title_fullStr |
Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil |
title_full_unstemmed |
Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil |
title_sort |
Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil |
author |
Kerbauy,G. |
author_facet |
Kerbauy,G. Perugini,M.R.E. Yamauchi,L.M. Yamada-Ogatta,S.F. |
author_role |
author |
author2 |
Perugini,M.R.E. Yamauchi,L.M. Yamada-Ogatta,S.F. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Kerbauy,G. Perugini,M.R.E. Yamauchi,L.M. Yamada-Ogatta,S.F. |
dc.subject.por.fl_str_mv |
Enterococcus faecium Vancomycin Dependence vanA genotype Virulence factors |
topic |
Enterococcus faecium Vancomycin Dependence vanA genotype Virulence factors |
description |
In this study, we report the characterization of a strain of Enterococcus faecium vanA, which grows only in the presence of vancomycin (VDEfm-UEL). The bacterium was isolated from the feces of a female patient who had undergone surgical treatment of Reinke’s edema and was receiving intravenous vancomycin therapy for infection with methicillin/oxacillin-resistant Staphylococcus aureus, a postoperative complication. Antimicrobial dependence was further confirmed by the vancomycin E-test. VDEfm-UEL was also shown to be resistant to ampicillin, ciprofloxacin, chloramphenicol, erythromycin, levofloxacin, penicillin, rifampicin, and teicoplanin. The putative virulence genes efaA, gelE and esp were detected by PCR. The ddl gene from VDEfm-UEL was cloned and sequenced. Vancomycin dependence seems to be associated with the insertion of a nucleotide in that sequence, which results in a frame-shift mutation, introducing a premature stop codon. This is the first report of vancomycin-dependent E. faecium isolation in a university hospital in Brazil. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2011007500006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.44 n.3 2011 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302939914043392 |