Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis

Detalhes bibliográficos
Autor(a) principal: Silva,J.A.X.
Data de Publicação: 2020
Outros Autores: Albertini,A.V.P., Fonseca,C.S.M., Silva,D.C.N., Carvalho,V.C.O., Lima,V.L.M., Fontes,A., Costa,E.V.L., Nogueira,R.A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000800601
Resumo: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which can cause lipid changes in the erythrocyte membrane. Optical tweezers were used to characterize rheological changes in erythrocytes from patients with leptospirosis in the late stage. Biochemical methods were also used for quantification of plasma lipid, erythrocyte membrane lipid, and evaluation of liver function. Our data showed that the mean elastic constant of erythrocytes from patients with leptospirosis was around 67% higher than the control (healthy individuals), indicating that patient’s erythrocytes were less elastic. In individuals with leptospirosis, several alterations in relation to control were observed in the plasma lipids, however, in the erythrocyte membrane, only phosphatidylcholine showed a significant difference compared to control, increasing around 41%. With respect to the evaluation of liver function of individuals with leptospirosis, there was a significant increase in levels of alanine transaminase (154%) and aspartate transaminase (150%), whereas albumin was 43.8% lower than control (P<0.01). The lecithin-cholesterol acyltransferase fractional activity was 3.6 times lower in individuals with leptospirosis than in the healthy individuals (P<0.01). The decrease of the erythrocyte elasticity may be related to the changes of erythrocyte membrane phospholipids composition caused by disturbances that occur during human leptospirosis, with phosphatidylcholine being a strong candidate in the erythrocyte rheological changes.
id ABDC-1_7dcded4b89dfaa0b3cdcd750dcd1b158
oai_identifier_str oai:scielo:S0100-879X2020000800601
network_acronym_str ABDC-1
network_name_str Brazilian Journal of Medical and Biological Research
repository_id_str
spelling Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosisLeptospirosisLipid composition of membraneErythrocyte elasticityOptical tweezersErythrocyte membraneLeptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which can cause lipid changes in the erythrocyte membrane. Optical tweezers were used to characterize rheological changes in erythrocytes from patients with leptospirosis in the late stage. Biochemical methods were also used for quantification of plasma lipid, erythrocyte membrane lipid, and evaluation of liver function. Our data showed that the mean elastic constant of erythrocytes from patients with leptospirosis was around 67% higher than the control (healthy individuals), indicating that patient’s erythrocytes were less elastic. In individuals with leptospirosis, several alterations in relation to control were observed in the plasma lipids, however, in the erythrocyte membrane, only phosphatidylcholine showed a significant difference compared to control, increasing around 41%. With respect to the evaluation of liver function of individuals with leptospirosis, there was a significant increase in levels of alanine transaminase (154%) and aspartate transaminase (150%), whereas albumin was 43.8% lower than control (P<0.01). The lecithin-cholesterol acyltransferase fractional activity was 3.6 times lower in individuals with leptospirosis than in the healthy individuals (P<0.01). The decrease of the erythrocyte elasticity may be related to the changes of erythrocyte membrane phospholipids composition caused by disturbances that occur during human leptospirosis, with phosphatidylcholine being a strong candidate in the erythrocyte rheological changes.Associação Brasileira de Divulgação Científica2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000800601Brazilian Journal of Medical and Biological Research v.53 n.8 2020reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20209268info:eu-repo/semantics/openAccessSilva,J.A.X.Albertini,A.V.P.Fonseca,C.S.M.Silva,D.C.N.Carvalho,V.C.O.Lima,V.L.M.Fontes,A.Costa,E.V.L.Nogueira,R.A.eng2020-06-16T00:00:00Zoai:scielo:S0100-879X2020000800601Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2020-06-16T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis
title Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis
spellingShingle Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis
Silva,J.A.X.
Leptospirosis
Lipid composition of membrane
Erythrocyte elasticity
Optical tweezers
Erythrocyte membrane
title_short Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis
title_full Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis
title_fullStr Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis
title_full_unstemmed Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis
title_sort Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis
author Silva,J.A.X.
author_facet Silva,J.A.X.
Albertini,A.V.P.
Fonseca,C.S.M.
Silva,D.C.N.
Carvalho,V.C.O.
Lima,V.L.M.
Fontes,A.
Costa,E.V.L.
Nogueira,R.A.
author_role author
author2 Albertini,A.V.P.
Fonseca,C.S.M.
Silva,D.C.N.
Carvalho,V.C.O.
Lima,V.L.M.
Fontes,A.
Costa,E.V.L.
Nogueira,R.A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,J.A.X.
Albertini,A.V.P.
Fonseca,C.S.M.
Silva,D.C.N.
Carvalho,V.C.O.
Lima,V.L.M.
Fontes,A.
Costa,E.V.L.
Nogueira,R.A.
dc.subject.por.fl_str_mv Leptospirosis
Lipid composition of membrane
Erythrocyte elasticity
Optical tweezers
Erythrocyte membrane
topic Leptospirosis
Lipid composition of membrane
Erythrocyte elasticity
Optical tweezers
Erythrocyte membrane
description Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which can cause lipid changes in the erythrocyte membrane. Optical tweezers were used to characterize rheological changes in erythrocytes from patients with leptospirosis in the late stage. Biochemical methods were also used for quantification of plasma lipid, erythrocyte membrane lipid, and evaluation of liver function. Our data showed that the mean elastic constant of erythrocytes from patients with leptospirosis was around 67% higher than the control (healthy individuals), indicating that patient’s erythrocytes were less elastic. In individuals with leptospirosis, several alterations in relation to control were observed in the plasma lipids, however, in the erythrocyte membrane, only phosphatidylcholine showed a significant difference compared to control, increasing around 41%. With respect to the evaluation of liver function of individuals with leptospirosis, there was a significant increase in levels of alanine transaminase (154%) and aspartate transaminase (150%), whereas albumin was 43.8% lower than control (P<0.01). The lecithin-cholesterol acyltransferase fractional activity was 3.6 times lower in individuals with leptospirosis than in the healthy individuals (P<0.01). The decrease of the erythrocyte elasticity may be related to the changes of erythrocyte membrane phospholipids composition caused by disturbances that occur during human leptospirosis, with phosphatidylcholine being a strong candidate in the erythrocyte rheological changes.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000800601
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000800601
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1414-431x20209268
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.53 n.8 2020
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
_version_ 1754302947672457216