Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000800601 |
Resumo: | Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which can cause lipid changes in the erythrocyte membrane. Optical tweezers were used to characterize rheological changes in erythrocytes from patients with leptospirosis in the late stage. Biochemical methods were also used for quantification of plasma lipid, erythrocyte membrane lipid, and evaluation of liver function. Our data showed that the mean elastic constant of erythrocytes from patients with leptospirosis was around 67% higher than the control (healthy individuals), indicating that patient’s erythrocytes were less elastic. In individuals with leptospirosis, several alterations in relation to control were observed in the plasma lipids, however, in the erythrocyte membrane, only phosphatidylcholine showed a significant difference compared to control, increasing around 41%. With respect to the evaluation of liver function of individuals with leptospirosis, there was a significant increase in levels of alanine transaminase (154%) and aspartate transaminase (150%), whereas albumin was 43.8% lower than control (P<0.01). The lecithin-cholesterol acyltransferase fractional activity was 3.6 times lower in individuals with leptospirosis than in the healthy individuals (P<0.01). The decrease of the erythrocyte elasticity may be related to the changes of erythrocyte membrane phospholipids composition caused by disturbances that occur during human leptospirosis, with phosphatidylcholine being a strong candidate in the erythrocyte rheological changes. |
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Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosisLeptospirosisLipid composition of membraneErythrocyte elasticityOptical tweezersErythrocyte membraneLeptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which can cause lipid changes in the erythrocyte membrane. Optical tweezers were used to characterize rheological changes in erythrocytes from patients with leptospirosis in the late stage. Biochemical methods were also used for quantification of plasma lipid, erythrocyte membrane lipid, and evaluation of liver function. Our data showed that the mean elastic constant of erythrocytes from patients with leptospirosis was around 67% higher than the control (healthy individuals), indicating that patient’s erythrocytes were less elastic. In individuals with leptospirosis, several alterations in relation to control were observed in the plasma lipids, however, in the erythrocyte membrane, only phosphatidylcholine showed a significant difference compared to control, increasing around 41%. With respect to the evaluation of liver function of individuals with leptospirosis, there was a significant increase in levels of alanine transaminase (154%) and aspartate transaminase (150%), whereas albumin was 43.8% lower than control (P<0.01). The lecithin-cholesterol acyltransferase fractional activity was 3.6 times lower in individuals with leptospirosis than in the healthy individuals (P<0.01). The decrease of the erythrocyte elasticity may be related to the changes of erythrocyte membrane phospholipids composition caused by disturbances that occur during human leptospirosis, with phosphatidylcholine being a strong candidate in the erythrocyte rheological changes.Associação Brasileira de Divulgação Científica2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000800601Brazilian Journal of Medical and Biological Research v.53 n.8 2020reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20209268info:eu-repo/semantics/openAccessSilva,J.A.X.Albertini,A.V.P.Fonseca,C.S.M.Silva,D.C.N.Carvalho,V.C.O.Lima,V.L.M.Fontes,A.Costa,E.V.L.Nogueira,R.A.eng2020-06-16T00:00:00Zoai:scielo:S0100-879X2020000800601Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2020-06-16T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis |
title |
Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis |
spellingShingle |
Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis Silva,J.A.X. Leptospirosis Lipid composition of membrane Erythrocyte elasticity Optical tweezers Erythrocyte membrane |
title_short |
Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis |
title_full |
Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis |
title_fullStr |
Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis |
title_full_unstemmed |
Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis |
title_sort |
Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis |
author |
Silva,J.A.X. |
author_facet |
Silva,J.A.X. Albertini,A.V.P. Fonseca,C.S.M. Silva,D.C.N. Carvalho,V.C.O. Lima,V.L.M. Fontes,A. Costa,E.V.L. Nogueira,R.A. |
author_role |
author |
author2 |
Albertini,A.V.P. Fonseca,C.S.M. Silva,D.C.N. Carvalho,V.C.O. Lima,V.L.M. Fontes,A. Costa,E.V.L. Nogueira,R.A. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,J.A.X. Albertini,A.V.P. Fonseca,C.S.M. Silva,D.C.N. Carvalho,V.C.O. Lima,V.L.M. Fontes,A. Costa,E.V.L. Nogueira,R.A. |
dc.subject.por.fl_str_mv |
Leptospirosis Lipid composition of membrane Erythrocyte elasticity Optical tweezers Erythrocyte membrane |
topic |
Leptospirosis Lipid composition of membrane Erythrocyte elasticity Optical tweezers Erythrocyte membrane |
description |
Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which can cause lipid changes in the erythrocyte membrane. Optical tweezers were used to characterize rheological changes in erythrocytes from patients with leptospirosis in the late stage. Biochemical methods were also used for quantification of plasma lipid, erythrocyte membrane lipid, and evaluation of liver function. Our data showed that the mean elastic constant of erythrocytes from patients with leptospirosis was around 67% higher than the control (healthy individuals), indicating that patient’s erythrocytes were less elastic. In individuals with leptospirosis, several alterations in relation to control were observed in the plasma lipids, however, in the erythrocyte membrane, only phosphatidylcholine showed a significant difference compared to control, increasing around 41%. With respect to the evaluation of liver function of individuals with leptospirosis, there was a significant increase in levels of alanine transaminase (154%) and aspartate transaminase (150%), whereas albumin was 43.8% lower than control (P<0.01). The lecithin-cholesterol acyltransferase fractional activity was 3.6 times lower in individuals with leptospirosis than in the healthy individuals (P<0.01). The decrease of the erythrocyte elasticity may be related to the changes of erythrocyte membrane phospholipids composition caused by disturbances that occur during human leptospirosis, with phosphatidylcholine being a strong candidate in the erythrocyte rheological changes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000800601 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000800601 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431x20209268 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.53 n.8 2020 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302947672457216 |