The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013 |
Resumo: | F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this method economically attractive. |
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Brazilian Journal of Medical and Biological Research |
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The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISAfilter paperpolyvinyl alcoholglutaraldehydeELISAdot-ELISAplagueF1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this method economically attractive.Associação Brasileira de Divulgação Científica2000-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013Brazilian Journal of Medical and Biological Research v.33 n.7 2000reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2000000700013info:eu-repo/semantics/openAccessBarbosa,G.H.T.S.Santana,E.M.Almeida,A.M.P.Araujo,A.M.Fatibello-Filho,O.Carvalho Jr.,L.B.eng2000-06-14T00:00:00Zoai:scielo:S0100-879X2000000700013Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2000-06-14T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA |
title |
The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA |
spellingShingle |
The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA Barbosa,G.H.T.S. filter paper polyvinyl alcohol glutaraldehyde ELISA dot-ELISA plague |
title_short |
The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA |
title_full |
The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA |
title_fullStr |
The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA |
title_full_unstemmed |
The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA |
title_sort |
The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA |
author |
Barbosa,G.H.T.S. |
author_facet |
Barbosa,G.H.T.S. Santana,E.M. Almeida,A.M.P. Araujo,A.M. Fatibello-Filho,O. Carvalho Jr.,L.B. |
author_role |
author |
author2 |
Santana,E.M. Almeida,A.M.P. Araujo,A.M. Fatibello-Filho,O. Carvalho Jr.,L.B. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Barbosa,G.H.T.S. Santana,E.M. Almeida,A.M.P. Araujo,A.M. Fatibello-Filho,O. Carvalho Jr.,L.B. |
dc.subject.por.fl_str_mv |
filter paper polyvinyl alcohol glutaraldehyde ELISA dot-ELISA plague |
topic |
filter paper polyvinyl alcohol glutaraldehyde ELISA dot-ELISA plague |
description |
F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this method economically attractive. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2000000700013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.33 n.7 2000 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302930515656704 |