Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas

Detalhes bibliográficos
Autor(a) principal: Veiga-Castelli,L.C.
Data de Publicação: 2010
Outros Autores: Rosa e Silva,J.C., Meola,J., Ferriani,R.A., Yoshimoto,M., Santos,S.A., Squire,J.A., Martelli,L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800014
Resumo: Endometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to detect chromosomal imbalances by comparative genomic hybridization (CGH) in ectopic tissue samples from ovarian endometriomas and eutopic tissue from the same patients. We evaluated 10 ovarian endometriotic tissues and 10 eutopic endometrial tissues by metaphase CGH. CGH was prepared with normal and test DNA enzymatically digested, ligated to adaptors and amplified by PCR. A second PCR was performed for DNA labeling. Equal amounts of both normal and test-labeled DNA were hybridized in human normal metaphases. The Isis FISH Imaging System V 5.0 software was used for chromosome analysis. In both eutopic and ectopic groups, 4/10 samples presented chromosomal alterations, mainly chromosomal gains. CGH identified 11q12.3-q13.1, 17p11.1-p12, 17q25.3-qter, and 19p as critical regions. Genomic imbalances in 11q, 17p, 17q, and 19p were detected in normal eutopic and/or ectopic endometrium from women with ovarian endometriosis. These regions contain genes such as POLR2G, MXRA7 and UBA52 involved in biological processes that may lead to the establishment and maintenance of endometriotic implants. This genomic imbalance may affect genes in which dysregulation impacts both eutopic and ectopic endometrium.
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spelling Genomic alterations detected by comparative genomic hybridization in ovarian endometriomasEndometriomaEndometriosisComparative genomic hybridizationChromosomal imbalancesEndometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to detect chromosomal imbalances by comparative genomic hybridization (CGH) in ectopic tissue samples from ovarian endometriomas and eutopic tissue from the same patients. We evaluated 10 ovarian endometriotic tissues and 10 eutopic endometrial tissues by metaphase CGH. CGH was prepared with normal and test DNA enzymatically digested, ligated to adaptors and amplified by PCR. A second PCR was performed for DNA labeling. Equal amounts of both normal and test-labeled DNA were hybridized in human normal metaphases. The Isis FISH Imaging System V 5.0 software was used for chromosome analysis. In both eutopic and ectopic groups, 4/10 samples presented chromosomal alterations, mainly chromosomal gains. CGH identified 11q12.3-q13.1, 17p11.1-p12, 17q25.3-qter, and 19p as critical regions. Genomic imbalances in 11q, 17p, 17q, and 19p were detected in normal eutopic and/or ectopic endometrium from women with ovarian endometriosis. These regions contain genes such as POLR2G, MXRA7 and UBA52 involved in biological processes that may lead to the establishment and maintenance of endometriotic implants. This genomic imbalance may affect genes in which dysregulation impacts both eutopic and ectopic endometrium.Associação Brasileira de Divulgação Científica2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800014Brazilian Journal of Medical and Biological Research v.43 n.8 2010reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2010007500072info:eu-repo/semantics/openAccessVeiga-Castelli,L.C.Rosa e Silva,J.C.Meola,J.Ferriani,R.A.Yoshimoto,M.Santos,S.A.Squire,J.A.Martelli,L.eng2010-08-17T00:00:00Zoai:scielo:S0100-879X2010000800014Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2010-08-17T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
title Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
spellingShingle Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
Veiga-Castelli,L.C.
Endometrioma
Endometriosis
Comparative genomic hybridization
Chromosomal imbalances
title_short Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
title_full Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
title_fullStr Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
title_full_unstemmed Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
title_sort Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
author Veiga-Castelli,L.C.
author_facet Veiga-Castelli,L.C.
Rosa e Silva,J.C.
Meola,J.
Ferriani,R.A.
Yoshimoto,M.
Santos,S.A.
Squire,J.A.
Martelli,L.
author_role author
author2 Rosa e Silva,J.C.
Meola,J.
Ferriani,R.A.
Yoshimoto,M.
Santos,S.A.
Squire,J.A.
Martelli,L.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Veiga-Castelli,L.C.
Rosa e Silva,J.C.
Meola,J.
Ferriani,R.A.
Yoshimoto,M.
Santos,S.A.
Squire,J.A.
Martelli,L.
dc.subject.por.fl_str_mv Endometrioma
Endometriosis
Comparative genomic hybridization
Chromosomal imbalances
topic Endometrioma
Endometriosis
Comparative genomic hybridization
Chromosomal imbalances
description Endometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to detect chromosomal imbalances by comparative genomic hybridization (CGH) in ectopic tissue samples from ovarian endometriomas and eutopic tissue from the same patients. We evaluated 10 ovarian endometriotic tissues and 10 eutopic endometrial tissues by metaphase CGH. CGH was prepared with normal and test DNA enzymatically digested, ligated to adaptors and amplified by PCR. A second PCR was performed for DNA labeling. Equal amounts of both normal and test-labeled DNA were hybridized in human normal metaphases. The Isis FISH Imaging System V 5.0 software was used for chromosome analysis. In both eutopic and ectopic groups, 4/10 samples presented chromosomal alterations, mainly chromosomal gains. CGH identified 11q12.3-q13.1, 17p11.1-p12, 17q25.3-qter, and 19p as critical regions. Genomic imbalances in 11q, 17p, 17q, and 19p were detected in normal eutopic and/or ectopic endometrium from women with ovarian endometriosis. These regions contain genes such as POLR2G, MXRA7 and UBA52 involved in biological processes that may lead to the establishment and maintenance of endometriotic implants. This genomic imbalance may affect genes in which dysregulation impacts both eutopic and ectopic endometrium.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2010007500072
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.43 n.8 2010
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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