Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600613 |
Resumo: | The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats. |
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Brazilian Journal of Medical and Biological Research |
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Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar ratsDevelopmentEpigeneticsFertilityGestationIntrauterine growthNutritionThe aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats.Associação Brasileira de Divulgação Científica2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600613Brazilian Journal of Medical and Biological Research v.51 n.6 2018reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20186602info:eu-repo/semantics/openAccessBarros,M.A.V.Andrade,E.B.Barros,R.G.N.Costa,I.K.M.Costa,I.C.L.Vitorino,G.F.A.Andrade,J.J.C.Paulino-Silva,K.M.Nogueira,V.O.de Brito Alves,J.L.Costa-Silva,J.H.eng2019-03-19T00:00:00Zoai:scielo:S0100-879X2018000600613Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2019-03-19T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats |
title |
Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats |
spellingShingle |
Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats Barros,M.A.V. Development Epigenetics Fertility Gestation Intrauterine growth Nutrition |
title_short |
Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats |
title_full |
Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats |
title_fullStr |
Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats |
title_full_unstemmed |
Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats |
title_sort |
Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats |
author |
Barros,M.A.V. |
author_facet |
Barros,M.A.V. Andrade,E.B. Barros,R.G.N. Costa,I.K.M. Costa,I.C.L. Vitorino,G.F.A. Andrade,J.J.C. Paulino-Silva,K.M. Nogueira,V.O. de Brito Alves,J.L. Costa-Silva,J.H. |
author_role |
author |
author2 |
Andrade,E.B. Barros,R.G.N. Costa,I.K.M. Costa,I.C.L. Vitorino,G.F.A. Andrade,J.J.C. Paulino-Silva,K.M. Nogueira,V.O. de Brito Alves,J.L. Costa-Silva,J.H. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Barros,M.A.V. Andrade,E.B. Barros,R.G.N. Costa,I.K.M. Costa,I.C.L. Vitorino,G.F.A. Andrade,J.J.C. Paulino-Silva,K.M. Nogueira,V.O. de Brito Alves,J.L. Costa-Silva,J.H. |
dc.subject.por.fl_str_mv |
Development Epigenetics Fertility Gestation Intrauterine growth Nutrition |
topic |
Development Epigenetics Fertility Gestation Intrauterine growth Nutrition |
description |
The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600613 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600613 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431x20186602 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.51 n.6 2018 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
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1754302946352300032 |