Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse

Detalhes bibliográficos
Autor(a) principal: Díaz-Corrales,F.J.
Data de Publicação: 2004
Outros Autores: Colasante,C., Contreras,Q., Puig,M., Serrano,J.A., Hernández,L., Beaman,B.L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400011
Resumo: Parkinson's disease, a major neurodegenerative disorder in humans whose etiology is unknown, may be associated with some environmental factors. Nocardia otitidiscaviarum (GAM-5) isolated from a patient with an actinomycetoma produced signs similar to Parkinson's disease following iv injection into NMRI mice. NMRI mice were infected intravenously with a non-lethal dose of 5 x 10(6) colony forming units of N. otitidiscaviarum (GAM-5). Fourteen days after bacterial infection, most of the 60 mice injected exhibited parkinsonian features characterized by vertical head tremor, akinesia/bradykinesia, flexed posture and postural instability. There was a peak of nocardial growth in the brain during the first 24 h followed by a decrease, so that by 14 days nocardiae could no longer be cultured. At 24 h after infection, Gram staining showed nocardiae in neurons in the substantia nigra and occasionally in the brain parenchyma in the frontal and parietal cortex. At 21 days post-infection, tyrosine hydroxylase immunolabeling showed a 58% reduction of tyrosine hydroxylase in the substantia nigra, and a 35% reduction of tyrosine hydroxylase in the ventral tegmental region. Dopamine levels were reduced from 110 ± 32.5 to 58 ± 16.5 ng/mg protein (47.2% reduction) in brain from infected mice exhibiting impaired movements, whereas serotonin levels were unchanged (191 ± 44 protein in control and 175 ± 39 ng/mg protein in injected mice). At later times, intraneuronal inclusion bodies were observed in the substantia nigra. Our observations emphasize the need for further studies of the potential association between Parkinson's disease or parkinsonism-like disease and exposure to various nocardial species.
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spelling Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouseNocardia otitidiscaviarum (GAM-5)Parkinson's diseaseSubstantia nigraBradykinesiaDopamineTyrosine hydroxylaseParkinson's disease, a major neurodegenerative disorder in humans whose etiology is unknown, may be associated with some environmental factors. Nocardia otitidiscaviarum (GAM-5) isolated from a patient with an actinomycetoma produced signs similar to Parkinson's disease following iv injection into NMRI mice. NMRI mice were infected intravenously with a non-lethal dose of 5 x 10(6) colony forming units of N. otitidiscaviarum (GAM-5). Fourteen days after bacterial infection, most of the 60 mice injected exhibited parkinsonian features characterized by vertical head tremor, akinesia/bradykinesia, flexed posture and postural instability. There was a peak of nocardial growth in the brain during the first 24 h followed by a decrease, so that by 14 days nocardiae could no longer be cultured. At 24 h after infection, Gram staining showed nocardiae in neurons in the substantia nigra and occasionally in the brain parenchyma in the frontal and parietal cortex. At 21 days post-infection, tyrosine hydroxylase immunolabeling showed a 58% reduction of tyrosine hydroxylase in the substantia nigra, and a 35% reduction of tyrosine hydroxylase in the ventral tegmental region. Dopamine levels were reduced from 110 ± 32.5 to 58 ± 16.5 ng/mg protein (47.2% reduction) in brain from infected mice exhibiting impaired movements, whereas serotonin levels were unchanged (191 ± 44 protein in control and 175 ± 39 ng/mg protein in injected mice). At later times, intraneuronal inclusion bodies were observed in the substantia nigra. Our observations emphasize the need for further studies of the potential association between Parkinson's disease or parkinsonism-like disease and exposure to various nocardial species.Associação Brasileira de Divulgação Científica2004-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400011Brazilian Journal of Medical and Biological Research v.37 n.4 2004reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2004000400011info:eu-repo/semantics/openAccessDíaz-Corrales,F.J.Colasante,C.Contreras,Q.Puig,M.Serrano,J.A.Hernández,L.Beaman,B.L.eng2004-04-22T00:00:00Zoai:scielo:S0100-879X2004000400011Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2004-04-22T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse
title Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse
spellingShingle Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse
Díaz-Corrales,F.J.
Nocardia otitidiscaviarum (GAM-5)
Parkinson's disease
Substantia nigra
Bradykinesia
Dopamine
Tyrosine hydroxylase
title_short Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse
title_full Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse
title_fullStr Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse
title_full_unstemmed Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse
title_sort Nocardia otitidiscaviarum (GAM-5) induces parkinsonian-like alterations in mouse
author Díaz-Corrales,F.J.
author_facet Díaz-Corrales,F.J.
Colasante,C.
Contreras,Q.
Puig,M.
Serrano,J.A.
Hernández,L.
Beaman,B.L.
author_role author
author2 Colasante,C.
Contreras,Q.
Puig,M.
Serrano,J.A.
Hernández,L.
Beaman,B.L.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Díaz-Corrales,F.J.
Colasante,C.
Contreras,Q.
Puig,M.
Serrano,J.A.
Hernández,L.
Beaman,B.L.
dc.subject.por.fl_str_mv Nocardia otitidiscaviarum (GAM-5)
Parkinson's disease
Substantia nigra
Bradykinesia
Dopamine
Tyrosine hydroxylase
topic Nocardia otitidiscaviarum (GAM-5)
Parkinson's disease
Substantia nigra
Bradykinesia
Dopamine
Tyrosine hydroxylase
description Parkinson's disease, a major neurodegenerative disorder in humans whose etiology is unknown, may be associated with some environmental factors. Nocardia otitidiscaviarum (GAM-5) isolated from a patient with an actinomycetoma produced signs similar to Parkinson's disease following iv injection into NMRI mice. NMRI mice were infected intravenously with a non-lethal dose of 5 x 10(6) colony forming units of N. otitidiscaviarum (GAM-5). Fourteen days after bacterial infection, most of the 60 mice injected exhibited parkinsonian features characterized by vertical head tremor, akinesia/bradykinesia, flexed posture and postural instability. There was a peak of nocardial growth in the brain during the first 24 h followed by a decrease, so that by 14 days nocardiae could no longer be cultured. At 24 h after infection, Gram staining showed nocardiae in neurons in the substantia nigra and occasionally in the brain parenchyma in the frontal and parietal cortex. At 21 days post-infection, tyrosine hydroxylase immunolabeling showed a 58% reduction of tyrosine hydroxylase in the substantia nigra, and a 35% reduction of tyrosine hydroxylase in the ventral tegmental region. Dopamine levels were reduced from 110 ± 32.5 to 58 ± 16.5 ng/mg protein (47.2% reduction) in brain from infected mice exhibiting impaired movements, whereas serotonin levels were unchanged (191 ± 44 protein in control and 175 ± 39 ng/mg protein in injected mice). At later times, intraneuronal inclusion bodies were observed in the substantia nigra. Our observations emphasize the need for further studies of the potential association between Parkinson's disease or parkinsonism-like disease and exposure to various nocardial species.
publishDate 2004
dc.date.none.fl_str_mv 2004-04-01
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400011
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2004000400011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.37 n.4 2004
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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