Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation

Detalhes bibliográficos
Autor(a) principal: Xu,Sha
Data de Publicação: 2021
Outros Autores: Xiong,Jun, Wu,Minjuan, Yang,Yu, Jiang,Junfeng, Ni,Haitao, Zhao,Yunpeng, Wang,Yue
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000200608
Resumo: Severe blockage in myeloid differentiation is the hallmark of acute myeloid leukemia (AML). Trdmt1 plays an important role in hematopoiesis. However, little is known about the function of Trdmt1 in AML cell differentiation. In the present study, Trdmt1 was up-regulated and miR-181a was down-regulated significantly during human leukemia HL-60 cell differentiation after TAT-CT3 fusion protein treatment. Accordingly, miR-181a overexpression in HL-60 cells inhibited granulocytic maturation. In addition, our “rescue” assay demonstrated that Trdmt1 3′-untranslated region promoted myeloid differentiation of HL-60 cells by sequestering miR-181a and up-regulating C/EBPα (a critical factor for normal myelopoiesis) via its competing endogenous RNA (ceRNA) activity on miR-181a. These findings revealed an unrecognized role of Trdmt1 as a potential ceRNA for therapeutic targets in AML.
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spelling Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiationAcute myeloid leukemiaTrdmt1ceRNAmiRNA-181aC/EBPαSevere blockage in myeloid differentiation is the hallmark of acute myeloid leukemia (AML). Trdmt1 plays an important role in hematopoiesis. However, little is known about the function of Trdmt1 in AML cell differentiation. In the present study, Trdmt1 was up-regulated and miR-181a was down-regulated significantly during human leukemia HL-60 cell differentiation after TAT-CT3 fusion protein treatment. Accordingly, miR-181a overexpression in HL-60 cells inhibited granulocytic maturation. In addition, our “rescue” assay demonstrated that Trdmt1 3′-untranslated region promoted myeloid differentiation of HL-60 cells by sequestering miR-181a and up-regulating C/EBPα (a critical factor for normal myelopoiesis) via its competing endogenous RNA (ceRNA) activity on miR-181a. These findings revealed an unrecognized role of Trdmt1 as a potential ceRNA for therapeutic targets in AML.Associação Brasileira de Divulgação Científica2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000200608Brazilian Journal of Medical and Biological Research v.54 n.2 2021reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20209869info:eu-repo/semantics/openAccessXu,ShaXiong,JunWu,MinjuanYang,YuJiang,JunfengNi,HaitaoZhao,YunpengWang,Yueeng2020-12-07T00:00:00Zoai:scielo:S0100-879X2021000200608Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2020-12-07T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation
title Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation
spellingShingle Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation
Xu,Sha
Acute myeloid leukemia
Trdmt1
ceRNA
miRNA-181a
C/EBPα
title_short Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation
title_full Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation
title_fullStr Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation
title_full_unstemmed Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation
title_sort Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation
author Xu,Sha
author_facet Xu,Sha
Xiong,Jun
Wu,Minjuan
Yang,Yu
Jiang,Junfeng
Ni,Haitao
Zhao,Yunpeng
Wang,Yue
author_role author
author2 Xiong,Jun
Wu,Minjuan
Yang,Yu
Jiang,Junfeng
Ni,Haitao
Zhao,Yunpeng
Wang,Yue
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Xu,Sha
Xiong,Jun
Wu,Minjuan
Yang,Yu
Jiang,Junfeng
Ni,Haitao
Zhao,Yunpeng
Wang,Yue
dc.subject.por.fl_str_mv Acute myeloid leukemia
Trdmt1
ceRNA
miRNA-181a
C/EBPα
topic Acute myeloid leukemia
Trdmt1
ceRNA
miRNA-181a
C/EBPα
description Severe blockage in myeloid differentiation is the hallmark of acute myeloid leukemia (AML). Trdmt1 plays an important role in hematopoiesis. However, little is known about the function of Trdmt1 in AML cell differentiation. In the present study, Trdmt1 was up-regulated and miR-181a was down-regulated significantly during human leukemia HL-60 cell differentiation after TAT-CT3 fusion protein treatment. Accordingly, miR-181a overexpression in HL-60 cells inhibited granulocytic maturation. In addition, our “rescue” assay demonstrated that Trdmt1 3′-untranslated region promoted myeloid differentiation of HL-60 cells by sequestering miR-181a and up-regulating C/EBPα (a critical factor for normal myelopoiesis) via its competing endogenous RNA (ceRNA) activity on miR-181a. These findings revealed an unrecognized role of Trdmt1 as a potential ceRNA for therapeutic targets in AML.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000200608
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000200608
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1414-431x20209869
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.54 n.2 2021
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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