Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands

Detalhes bibliográficos
Autor(a) principal: Sotres-Vega,A.
Data de Publicação: 2009
Outros Autores: Baltazares-Lipp,M., Villalba-Caloca,J., Gaxiola-Gaxiola,M.O., Santibañez-Salgado,J.A., Olmos-Zúñiga,J.R., Jasso-Victoria,R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006
Resumo: Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to cryopreserved tracheal segments in which it remained high, due to the effect of storage in liquid nitrogen (P < 0.05, ANOVA and Tukey test). We conclude that apoptosis can be triggered in epithelial cells during tracheal graft harvesting even prior to cryopreservation, and although the epithelial caspase-3 immunoreactivity is reduced in tracheal cryopreservation, this could be explained by increased cell death. Apoptosis cannot be stopped during tracheal cryopreservation.
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spelling Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glandsTracheal cryopreservationEpitheliumCaspase-3Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to cryopreserved tracheal segments in which it remained high, due to the effect of storage in liquid nitrogen (P < 0.05, ANOVA and Tukey test). We conclude that apoptosis can be triggered in epithelial cells during tracheal graft harvesting even prior to cryopreservation, and although the epithelial caspase-3 immunoreactivity is reduced in tracheal cryopreservation, this could be explained by increased cell death. Apoptosis cannot be stopped during tracheal cryopreservation.Associação Brasileira de Divulgação Científica2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006Brazilian Journal of Medical and Biological Research v.42 n.12 2009reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2009007500009info:eu-repo/semantics/openAccessSotres-Vega,A.Baltazares-Lipp,M.Villalba-Caloca,J.Gaxiola-Gaxiola,M.O.Santibañez-Salgado,J.A.Olmos-Zúñiga,J.R.Jasso-Victoria,R.eng2009-12-04T00:00:00Zoai:scielo:S0100-879X2009001200006Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2009-12-04T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands
title Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands
spellingShingle Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands
Sotres-Vega,A.
Tracheal cryopreservation
Epithelium
Caspase-3
title_short Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands
title_full Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands
title_fullStr Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands
title_full_unstemmed Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands
title_sort Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands
author Sotres-Vega,A.
author_facet Sotres-Vega,A.
Baltazares-Lipp,M.
Villalba-Caloca,J.
Gaxiola-Gaxiola,M.O.
Santibañez-Salgado,J.A.
Olmos-Zúñiga,J.R.
Jasso-Victoria,R.
author_role author
author2 Baltazares-Lipp,M.
Villalba-Caloca,J.
Gaxiola-Gaxiola,M.O.
Santibañez-Salgado,J.A.
Olmos-Zúñiga,J.R.
Jasso-Victoria,R.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sotres-Vega,A.
Baltazares-Lipp,M.
Villalba-Caloca,J.
Gaxiola-Gaxiola,M.O.
Santibañez-Salgado,J.A.
Olmos-Zúñiga,J.R.
Jasso-Victoria,R.
dc.subject.por.fl_str_mv Tracheal cryopreservation
Epithelium
Caspase-3
topic Tracheal cryopreservation
Epithelium
Caspase-3
description Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to cryopreserved tracheal segments in which it remained high, due to the effect of storage in liquid nitrogen (P < 0.05, ANOVA and Tukey test). We conclude that apoptosis can be triggered in epithelial cells during tracheal graft harvesting even prior to cryopreservation, and although the epithelial caspase-3 immunoreactivity is reduced in tracheal cryopreservation, this could be explained by increased cell death. Apoptosis cannot be stopped during tracheal cryopreservation.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2009007500009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.42 n.12 2009
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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