Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006 |
Resumo: | Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to cryopreserved tracheal segments in which it remained high, due to the effect of storage in liquid nitrogen (P < 0.05, ANOVA and Tukey test). We conclude that apoptosis can be triggered in epithelial cells during tracheal graft harvesting even prior to cryopreservation, and although the epithelial caspase-3 immunoreactivity is reduced in tracheal cryopreservation, this could be explained by increased cell death. Apoptosis cannot be stopped during tracheal cryopreservation. |
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Brazilian Journal of Medical and Biological Research |
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Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glandsTracheal cryopreservationEpitheliumCaspase-3Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to cryopreserved tracheal segments in which it remained high, due to the effect of storage in liquid nitrogen (P < 0.05, ANOVA and Tukey test). We conclude that apoptosis can be triggered in epithelial cells during tracheal graft harvesting even prior to cryopreservation, and although the epithelial caspase-3 immunoreactivity is reduced in tracheal cryopreservation, this could be explained by increased cell death. Apoptosis cannot be stopped during tracheal cryopreservation.Associação Brasileira de Divulgação Científica2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006Brazilian Journal of Medical and Biological Research v.42 n.12 2009reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2009007500009info:eu-repo/semantics/openAccessSotres-Vega,A.Baltazares-Lipp,M.Villalba-Caloca,J.Gaxiola-Gaxiola,M.O.Santibañez-Salgado,J.A.Olmos-Zúñiga,J.R.Jasso-Victoria,R.eng2009-12-04T00:00:00Zoai:scielo:S0100-879X2009001200006Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2009-12-04T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands |
title |
Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands |
spellingShingle |
Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands Sotres-Vega,A. Tracheal cryopreservation Epithelium Caspase-3 |
title_short |
Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands |
title_full |
Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands |
title_fullStr |
Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands |
title_full_unstemmed |
Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands |
title_sort |
Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands |
author |
Sotres-Vega,A. |
author_facet |
Sotres-Vega,A. Baltazares-Lipp,M. Villalba-Caloca,J. Gaxiola-Gaxiola,M.O. Santibañez-Salgado,J.A. Olmos-Zúñiga,J.R. Jasso-Victoria,R. |
author_role |
author |
author2 |
Baltazares-Lipp,M. Villalba-Caloca,J. Gaxiola-Gaxiola,M.O. Santibañez-Salgado,J.A. Olmos-Zúñiga,J.R. Jasso-Victoria,R. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sotres-Vega,A. Baltazares-Lipp,M. Villalba-Caloca,J. Gaxiola-Gaxiola,M.O. Santibañez-Salgado,J.A. Olmos-Zúñiga,J.R. Jasso-Victoria,R. |
dc.subject.por.fl_str_mv |
Tracheal cryopreservation Epithelium Caspase-3 |
topic |
Tracheal cryopreservation Epithelium Caspase-3 |
description |
Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to cryopreserved tracheal segments in which it remained high, due to the effect of storage in liquid nitrogen (P < 0.05, ANOVA and Tukey test). We conclude that apoptosis can be triggered in epithelial cells during tracheal graft harvesting even prior to cryopreservation, and although the epithelial caspase-3 immunoreactivity is reduced in tracheal cryopreservation, this could be explained by increased cell death. Apoptosis cannot be stopped during tracheal cryopreservation. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2009007500009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.42 n.12 2009 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302937772851200 |